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The map label for this gene is ntaB [H]

Identifier: 116690595

GI number: 116690595

Start: 2850452

End: 2850961

Strand: Direct

Name: ntaB [H]

Synonym: Bcen2424_2575

Alternate gene names: 116690595

Gene position: 2850452-2850961 (Clockwise)

Preceding gene: 116690594

Following gene: 116690597

Centisome position: 81.82

GC content: 67.45

Gene sequence:

>510_bases
ATGAGCCACGCCAATCCGCCGGATTTCGACGCGGCCGCCTTCCGGCAGGCACTCGGCCAGTTCGCGACGGGCGTGACGGT
GATCACGACGCGCGCGCCGTCCGGCCAGCTGATCGGCATCACCGCCAGCTCGTTCAATTCGGTGTCGCTCGATCCGCCGC
TCGTGCTGTGGAGCCTCGCGCACAAATCGGCGTCGACGCCGGTGTTCCGCGGCAACAGCCATTACGTGGTGAACGTGCTC
GCGGCGTCGCAACTCGACCTGTGCAAGCGCTTCTCGACCTACAAGGGCGATCGTTTCGAAGGGATCGCGCACGCGGCCGG
CAACTCGGGAATGCCGGTGCTCGACGGCGCGCTCGCGTGGTTCGAATGCCACAACCGCAGCCGCTACGACGAAGGCGACC
ACGTGATCTTCGTCGGCGAAGTGGAACGCTGCGGCGTGCGCGCGGTATCGGAAACGGCCCCGCCGCTCGTGTTCCACGGC
GGCGGCTTCCACGGCCTCACACCGCTTTGA

Upstream 100 bases:

>100_bases
GCACCCCTTCCCAATCGACCGCGTTGTCATTTTTCGTCCGCCGCGCCCGTCACGCCCGTGTCGTGTCGCGCCCGCCCGTC
CCGTTCCGGATGGCTGCCCG

Downstream 100 bases:

>100_bases
TCGCCCCCGTGCGCGCCAGTCCGACCGGCGCGCCGGCTTCGTCCTTCAGCGTCTGCAGCACGATGTTCGACCGGATGTCC
ATCACGCCCGGCGCCTTGTA

Product: flavin reductase domain-containing protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MSHANPPDFDAAAFRQALGQFATGVTVITTRAPSGQLIGITASSFNSVSLDPPLVLWSLAHKSASTPVFRGNSHYVVNVL
AASQLDLCKRFSTYKGDRFEGIAHAAGNSGMPVLDGALAWFECHNRSRYDEGDHVIFVGEVERCGVRAVSETAPPLVFHG
GGFHGLTPL

Sequences:

>Translated_169_residues
MSHANPPDFDAAAFRQALGQFATGVTVITTRAPSGQLIGITASSFNSVSLDPPLVLWSLAHKSASTPVFRGNSHYVVNVL
AASQLDLCKRFSTYKGDRFEGIAHAAGNSGMPVLDGALAWFECHNRSRYDEGDHVIFVGEVERCGVRAVSETAPPLVFHG
GGFHGLTPL
>Mature_168_residues
SHANPPDFDAAAFRQALGQFATGVTVITTRAPSGQLIGITASSFNSVSLDPPLVLWSLAHKSASTPVFRGNSHYVVNVLA
ASQLDLCKRFSTYKGDRFEGIAHAAGNSGMPVLDGALAWFECHNRSRYDEGDHVIFVGEVERCGVRAVSETAPPLVFHGG
GFHGLTPL

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=161, Percent_Identity=31.6770186335404, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 17995; Mature: 17864

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHANPPDFDAAAFRQALGQFATGVTVITTRAPSGQLIGITASSFNSVSLDPPLVLWSLA
CCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEHH
HKSASTPVFRGNSHYVVNVLAASQLDLCKRFSTYKGDRFEGIAHAAGNSGMPVLDGALAW
HCCCCCCEEECCCCEEEEEECHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHE
FECHNRSRYDEGDHVIFVGEVERCGVRAVSETAPPLVFHGGGFHGLTPL
EHHCCCCCCCCCCEEEEEECCCCCCCEEHHCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
SHANPPDFDAAAFRQALGQFATGVTVITTRAPSGQLIGITASSFNSVSLDPPLVLWSLA
CCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEHH
HKSASTPVFRGNSHYVVNVLAASQLDLCKRFSTYKGDRFEGIAHAAGNSGMPVLDGALAW
HCCCCCCEEECCCCEEEEEECHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHE
FECHNRSRYDEGDHVIFVGEVERCGVRAVSETAPPLVFHGGGFHGLTPL
EHHCCCCCCCCCCEEEEEECCCCCCCEEHHCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]