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The map label for this gene is amiC [H]

Identifier: 116690583

GI number: 116690583

Start: 2838043

End: 2839557

Strand: Direct

Name: amiC [H]

Synonym: Bcen2424_2563

Alternate gene names: 116690583

Gene position: 2838043-2839557 (Clockwise)

Preceding gene: 116690582

Following gene: 116690585

Centisome position: 81.46

GC content: 69.04

Gene sequence:

>1515_bases
ATGTTGATCAAACCGTTCCGCTCGATCGAATCGGCGGCCACCGCGACGCACAACTGGCGGCGCCGTCAGATCCTGCGCGC
GGGGGCGTCGACGCTGGTGCTCGGTCTCGTCGCGCCGCGGCTCGCGCACGCGTCGTCGGTGCTCGGCGTGCGCGTGTGGC
CCGCACGCGATTACACGCGCGTCACGATCGAATCCGACCAGCCGCTGCAGAATAGCCAACAGCTGCTGCAGGGTCCCGAC
CGTCTCGTCGTCGACCTGAGCGGCCTCGACCTCGACCAGGCGCTGCGCGACCTCGTCTCGAAGATCGCGCCGAACGATCC
GCAGATCCAGTCGGTGCGCGTCGGCCAGTATCAGCCACACGTCGTGCGGATGGTGTTCGACCTGAAAGGCTCGGTGAAGC
CGCAGGTGTTCACGCTGCCGCCCGTCGGCACGTACAAGTACCGGCTGGTGTTCGACCTGTATCCGGCCGTCGCGCCCGAT
CCGCTGTCCGACCTGATCGCGCAGACGGAACGCAAGGAACAGGCGCTCAACGACACCGCGCGCGCGCAGCAGATGCAGCC
GCCGTCCACGCTGGCGGGCCCCGGCACCCCGCCGCCCGGCGCGGGCGACAACAGCGACGCATTCTTCCAGCGTTTCGCGC
AGAACACGCCGGCCACGCCGCACGCGCCGCCGGCCGCCGGCACGCCGTCGACGCCCGCGAAGCCGGCCGTCAAGCCGCCG
CCCGTCATCGCGCGCCGCGACGACAGCGACGACGACGGCGACACCTACAAGTTCACCGCGCCGAAATCCGGCAAGGGCGG
CACCGTGCGTCTGCTGACGGTCGCGATCGACCCGGGCCACGGCGGCGAGGATCCGGGCGCGATCGGCGGCAACGGCACGT
ACGAGAAGCACATCGCGCTCGACATCGCGAAGAAGCTGCGCGCGAAGATCGACGCCGCGCCGAACATGCGCGCGATGATG
ACGCGCGACGCCGACTTCTTCGTGCCGCTGAACGTGCGCGTGCAGAAGGCGCGCCGCGTCGGCGCGGATCTCTTCGTGTC
GATCCACGCGGATGCGTTCACGACGCCGTCCGCACGCGGCTCGTCGGTGTTCGCGCTGTCCGACCACGGCGCGACGAGCG
CCGCGGCGCGCTGGATGGCGAACAAGGAGAATTCGTCGGACCTGATCGGCGGCATCAACATCAAGACGGCGGACGCGGCC
GTGAACCGCGCGCTGTTCGACATGTCGACGACCGCGCAGATCCGCGACTCGCTGCGCTACGGCAACTACGTGCTGAAGGA
AGTCGGCGGCATCAACAAGCTGCACAAGGGCTCGGTCGAGCAGGCCGGGTTCGCGGTGCTGAAGGCGCCCGACATTCCGT
CGATCCTCGTCGAGACCGCGTTCATCAGCAACCCGGACGAAGAGCGCCGGCTCAACGACGACAGCTATCGCGACGAGATG
GCCGACGCGATCTTCCGCGGCATCAAGCGCTATTTCGCCGCGAATCCGCCGCTCGCGAAGAGCCGGATGGCCTGA

Upstream 100 bases:

>100_bases
TCGGCGTGCCCGATCTGGTTTTCTCGCTCGACGTGGATGGCGACGGCCGTGCCCTCACCGTCCGGGCGTACAGCGCTTCA
GGAAAGGCATGTCTCGAAAG

Downstream 100 bases:

>100_bases
GCGCCACCGCTTCCCCCCGCCGGCACGCAGTGCGTGCCGGCGCGTCACGACGCCGCGAAGCGGCTCAGCAGTTTCCCGCC
GAACACGTTCACCGCGAGCC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLVAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLLQGPD
RLVVDLSGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPD
PLSDLIAQTERKEQALNDTARAQQMQPPSTLAGPGTPPPGAGDNSDAFFQRFAQNTPATPHAPPAAGTPSTPAKPAVKPP
PVIARRDDSDDDGDTYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGNGTYEKHIALDIAKKLRAKIDAAPNMRAMM
TRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGATSAAARWMANKENSSDLIGGINIKTADAA
VNRALFDMSTTAQIRDSLRYGNYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERRLNDDSYRDEM
ADAIFRGIKRYFAANPPLAKSRMA

Sequences:

>Translated_504_residues
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLVAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLLQGPD
RLVVDLSGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPD
PLSDLIAQTERKEQALNDTARAQQMQPPSTLAGPGTPPPGAGDNSDAFFQRFAQNTPATPHAPPAAGTPSTPAKPAVKPP
PVIARRDDSDDDGDTYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGNGTYEKHIALDIAKKLRAKIDAAPNMRAMM
TRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGATSAAARWMANKENSSDLIGGINIKTADAA
VNRALFDMSTTAQIRDSLRYGNYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERRLNDDSYRDEM
ADAIFRGIKRYFAANPPLAKSRMA
>Mature_504_residues
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLVAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLLQGPD
RLVVDLSGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPD
PLSDLIAQTERKEQALNDTARAQQMQPPSTLAGPGTPPPGAGDNSDAFFQRFAQNTPATPHAPPAAGTPSTPAKPAVKPP
PVIARRDDSDDDGDTYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGNGTYEKHIALDIAKKLRAKIDAAPNMRAMM
TRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGATSAAARWMANKENSSDLIGGINIKTADAA
VNRALFDMSTTAQIRDSLRYGNYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERRLNDDSYRDEM
ADAIFRGIKRYFAANPPLAKSRMA

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=242, Percent_Identity=55.7851239669422, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=47.4358974358974, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1790611, Length=229, Percent_Identity=40.6113537117904, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 54434; Mature: 54434

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLVAPRLAHASSVLGVRVWPARDYTR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEE
VTIESDQPLQNSQQLLQGPDRLVVDLSGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPH
EEECCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
VVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPDPLSDLIAQTERKEQALNDTA
HEEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RAQQMQPPSTLAGPGTPPPGAGDNSDAFFQRFAQNTPATPHAPPAAGTPSTPAKPAVKPP
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PVIARRDDSDDDGDTYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGNGTYEKHIAL
CEEEECCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
DIAKKLRAKIDAAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARG
HHHHHHHHHHCCCCCCCEEEECCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCCCC
SSVFALSDHGATSAAARWMANKENSSDLIGGINIKTADAAVNRALFDMSTTAQIRDSLRY
CEEEEEECCCCHHHHHHHHCCCCCCCCEEECCEEEEHHHHHHHHHHCCCHHHHHHHHHHH
GNYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERRLNDDSYRDEM
HHHHHHHHCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCCHHHCCCCHHHHHHH
ADAIFRGIKRYFAANPPLAKSRMA
HHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLVAPRLAHASSVLGVRVWPARDYTR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEE
VTIESDQPLQNSQQLLQGPDRLVVDLSGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPH
EEECCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
VVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPDPLSDLIAQTERKEQALNDTA
HEEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RAQQMQPPSTLAGPGTPPPGAGDNSDAFFQRFAQNTPATPHAPPAAGTPSTPAKPAVKPP
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PVIARRDDSDDDGDTYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGNGTYEKHIAL
CEEEECCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
DIAKKLRAKIDAAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARG
HHHHHHHHHHCCCCCCCEEEECCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCCCC
SSVFALSDHGATSAAARWMANKENSSDLIGGINIKTADAAVNRALFDMSTTAQIRDSLRY
CEEEEEECCCCHHHHHHHHCCCCCCCCEEECCEEEEHHHHHHHHHHCCCHHHHHHHHHHH
GNYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERRLNDDSYRDEM
HHHHHHHHCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCCHHHCCCCHHHHHHH
ADAIFRGIKRYFAANPPLAKSRMA
HHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]