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The map label for this gene is purN [H]

Identifier: 116690530

GI number: 116690530

Start: 2780086

End: 2780748

Strand: Reverse

Name: purN [H]

Synonym: Bcen2424_2510

Alternate gene names: 116690530

Gene position: 2780748-2780086 (Counterclockwise)

Preceding gene: 116690536

Following gene: 116690529

Centisome position: 79.82

GC content: 67.72

Gene sequence:

>663_bases
ATGAAAAAACTCGTGATCCTGATTTCCGGTCGCGGCAGCAACATGGAGGCCATCGTCCGCGCGTGCGCGCAGGAACGCTG
GCCGGCCGAGATTGCCGCCGTGATCGCCAACCGGCCCGATGCGGCCGGACTGGCTTTTGCCGCGTCGCACGGGGTGGCGA
CGGCGGTGGTCGACCACCGGTCGTTCGACGGCCGCGACAGCTTCGACGCGGCGCTCGCCGCCGAAATCGACCGTTTTTCG
CCCGATCTCGTCGTTCTCGCCGGCTTCATGCGCATCCTGACGCCTGCATTCGTCAGACGATACGAGGGCCGGCTGCTCAA
CATCCATCCGTCGCTGCTGCCGAGCTTCAAGGGCATCCACACGCACCAGCAGGCGCTGGATGCGGGCGTCGCGCTGCATG
GCGCGAGCGTGCATTTCGTCATTCCCGAGCTCGACAGCGGCGCGATCGTCGCGCAGGGCGCGGTGCCGGTGCATGCCGGC
GACGACGCGGCCGCGCTCGCGCAGCGCGTGCTGACGGTCGAGCACGTGCTGTATCCGCGTGCGGTGCGCTGGTTCGTCGA
AGGGCGTCTGCGCCTCGAGGACGGTCGTGCAGTGGTCGCGCCCGAAGAGGCGCGCTGGATTTTCGCGGATCAACCGCAAA
CCGAAACGAGTGAGGGCGTATGA

Upstream 100 bases:

>100_bases
AATGGGCGGTGGGATGCCGCAAAGCAGAAGATTATAAACGTTCGAGCGGGCCGACGGCCGAGAAGGCGGGAAACGCGAGG
GCGAATGGTAAAATTCGCGG

Downstream 100 bases:

>100_bases
AGCTGCACGGATTCCTGATTGGCCAGACCGAAACCCTGCTCGCCGAGGTGCTGAAGTTCGCCGGCCCCGCCGACGCGACG
ACGAGCCGGTTCTTCCGCGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKKLVILISGRGSNMEAIVRACAQERWPAEIAAVIANRPDAAGLAFAASHGVATAVVDHRSFDGRDSFDAALAAEIDRFS
PDLVVLAGFMRILTPAFVRRYEGRLLNIHPSLLPSFKGIHTHQQALDAGVALHGASVHFVIPELDSGAIVAQGAVPVHAG
DDAAALAQRVLTVEHVLYPRAVRWFVEGRLRLEDGRAVVAPEEARWIFADQPQTETSEGV

Sequences:

>Translated_220_residues
MKKLVILISGRGSNMEAIVRACAQERWPAEIAAVIANRPDAAGLAFAASHGVATAVVDHRSFDGRDSFDAALAAEIDRFS
PDLVVLAGFMRILTPAFVRRYEGRLLNIHPSLLPSFKGIHTHQQALDAGVALHGASVHFVIPELDSGAIVAQGAVPVHAG
DDAAALAQRVLTVEHVLYPRAVRWFVEGRLRLEDGRAVVAPEEARWIFADQPQTETSEGV
>Mature_220_residues
MKKLVILISGRGSNMEAIVRACAQERWPAEIAAVIANRPDAAGLAFAASHGVATAVVDHRSFDGRDSFDAALAAEIDRFS
PDLVVLAGFMRILTPAFVRRYEGRLLNIHPSLLPSFKGIHTHQQALDAGVALHGASVHFVIPELDSGAIVAQGAVPVHAG
DDAAALAQRVLTVEHVLYPRAVRWFVEGRLRLEDGRAVVAPEEARWIFADQPQTETSEGV

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=202, Percent_Identity=43.5643564356436, Blast_Score=179, Evalue=1e-45,
Organism=Homo sapiens, GI209869995, Length=202, Percent_Identity=43.5643564356436, Blast_Score=179, Evalue=1e-45,
Organism=Homo sapiens, GI209869993, Length=202, Percent_Identity=43.5643564356436, Blast_Score=179, Evalue=1e-45,
Organism=Escherichia coli, GI1788846, Length=195, Percent_Identity=50.7692307692308, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1787483, Length=198, Percent_Identity=30.8080808080808, Blast_Score=97, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=39.4444444444444, Blast_Score=140, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6320616, Length=200, Percent_Identity=29.5, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24582400, Length=203, Percent_Identity=40.8866995073892, Blast_Score=161, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23679; Mature: 23679

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVILISGRGSNMEAIVRACAQERWPAEIAAVIANRPDAAGLAFAASHGVATAVVDHR
CCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEEECC
SFDGRDSFDAALAAEIDRFSPDLVVLAGFMRILTPAFVRRYEGRLLNIHPSLLPSFKGIH
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCCH
THQQALDAGVALHGASVHFVIPELDSGAIVAQGAVPVHAGDDAAALAQRVLTVEHVLYPR
HHHHHHHCCEEEECCEEEEEEEECCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHH
AVRWFVEGRLRLEDGRAVVAPEEARWIFADQPQTETSEGV
HHHHHHCCEEEEECCCEEECCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKKLVILISGRGSNMEAIVRACAQERWPAEIAAVIANRPDAAGLAFAASHGVATAVVDHR
CCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEEECC
SFDGRDSFDAALAAEIDRFSPDLVVLAGFMRILTPAFVRRYEGRLLNIHPSLLPSFKGIH
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCCH
THQQALDAGVALHGASVHFVIPELDSGAIVAQGAVPVHAGDDAAALAQRVLTVEHVLYPR
HHHHHHHCCEEEECCEEEEEEEECCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHH
AVRWFVEGRLRLEDGRAVVAPEEARWIFADQPQTETSEGV
HHHHHHCCEEEEECCCEEECCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]