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The map label for this gene is yusZ [H]

Identifier: 116690500

GI number: 116690500

Start: 2751975

End: 2752874

Strand: Direct

Name: yusZ [H]

Synonym: Bcen2424_2480

Alternate gene names: 116690500

Gene position: 2751975-2752874 (Clockwise)

Preceding gene: 116690499

Following gene: 116690502

Centisome position: 78.99

GC content: 72.33

Gene sequence:

>900_bases
ATGAAGGAAGTCGTCCTCGTCACCGGCGCGTCCAGCGGCTTCGGCTTGCTGACGGTCCAGGCGCTCGCGCGCGCCGGTCA
CACGGTCTATGCATCGATGCGCGAGCGCGCCGGGCGCAACGCGCCGCGCGCCGCCGCCGTCGCGGCCTATGCGCAGGAAC
ACGGCGTCGACCTGCGCAGCCTCGAACTCGACGTCGGCGACGACGCATCGGTCGACGCCGCCATCGAGCGCGTGATCACC
GACAACGGCCGTCTGGACGTCGTCGTCCACAACGCCGGCCACATGGTGTTCGGGCCGGCCGAAGCCTTCACGCCGGAGCA
GCTTGCGCAGCTCTACGACGTCAACGTCGTGTCGACCCAGCGCGTGAACCGCGCGGCGCTGCCGCACCTGCGCCGCCAGG
GCCGCGGCCTGCTCGTGTGGGTGTCGTCCTCGTCGGCGCGCGGCGGCACCCCGCCGTTCCTCGCCCCCTACTTCGCCGCG
AAAGCCGCGATGGACTCGCTCGCGGTGTCCTACGCCGCCGAACTCGCCCGCTGGGGCATCGAAACGTCGATCGTCGTGCC
GGGCGCGTTCACCCGAGGCACGAATCACTTCCTCCATGCGGGCAAGCCGGCCGATACCGCCGTGCAGGCTGCGTACGACA
GCGGGCCGTACGCGGGCGTCGCCGATCAGGCGTTGACGGGCCTCGCCGCGCTCGAACCGGCGGACGCCGACGCCGGCGCC
GTCGCCACGGCGATTGCCGAGCTCGTCGCGGCGCCGGCCGGCAAGCGCCCCTACCGCGTGTTCGTCGACCCGTCGCAGGA
CGGCGCCGAGGAAGTGTTCCGTGTCGGCGATCGCATCCGCCGCGAGATGTTCCGGAACATCGGGCTCGCCGATCTGCTCG
CGCCGCGTGTCGACGGCTGA

Upstream 100 bases:

>100_bases
CCGAACGGCGCGTGGATCAACGGCCAGATCCTGCGCGCCAACGGCGGCATGTGCTGACGCCCCCTCGCCGCCACCTCATC
TTTCTGCCAGGAGATCGCTC

Downstream 100 bases:

>100_bases
TTTCTTCGCGCGCCGGGCCGGCCGGCGCGCGTCAATCCCGCTCGCAGAAGCCGAGAATCGCGTCAGCGACCGCCGTCGCG
GATTCGCGCTGCGGGAAGTG

Product: short-chain dehydrogenase/reductase SDR

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: ORFA [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKEVVLVTGASSGFGLLTVQALARAGHTVYASMRERAGRNAPRAAAVAAYAQEHGVDLRSLELDVGDDASVDAAIERVIT
DNGRLDVVVHNAGHMVFGPAEAFTPEQLAQLYDVNVVSTQRVNRAALPHLRRQGRGLLVWVSSSSARGGTPPFLAPYFAA
KAAMDSLAVSYAAELARWGIETSIVVPGAFTRGTNHFLHAGKPADTAVQAAYDSGPYAGVADQALTGLAALEPADADAGA
VATAIAELVAAPAGKRPYRVFVDPSQDGAEEVFRVGDRIRREMFRNIGLADLLAPRVDG

Sequences:

>Translated_299_residues
MKEVVLVTGASSGFGLLTVQALARAGHTVYASMRERAGRNAPRAAAVAAYAQEHGVDLRSLELDVGDDASVDAAIERVIT
DNGRLDVVVHNAGHMVFGPAEAFTPEQLAQLYDVNVVSTQRVNRAALPHLRRQGRGLLVWVSSSSARGGTPPFLAPYFAA
KAAMDSLAVSYAAELARWGIETSIVVPGAFTRGTNHFLHAGKPADTAVQAAYDSGPYAGVADQALTGLAALEPADADAGA
VATAIAELVAAPAGKRPYRVFVDPSQDGAEEVFRVGDRIRREMFRNIGLADLLAPRVDG
>Mature_299_residues
MKEVVLVTGASSGFGLLTVQALARAGHTVYASMRERAGRNAPRAAAVAAYAQEHGVDLRSLELDVGDDASVDAAIERVIT
DNGRLDVVVHNAGHMVFGPAEAFTPEQLAQLYDVNVVSTQRVNRAALPHLRRQGRGLLVWVSSSSARGGTPPFLAPYFAA
KAAMDSLAVSYAAELARWGIETSIVVPGAFTRGTNHFLHAGKPADTAVQAAYDSGPYAGVADQALTGLAALEPADADAGA
VATAIAELVAAPAGKRPYRVFVDPSQDGAEEVFRVGDRIRREMFRNIGLADLLAPRVDG

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI223718074, Length=192, Percent_Identity=39.0625, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI7657478, Length=187, Percent_Identity=32.620320855615, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI119392083, Length=190, Percent_Identity=32.6315789473684, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI32483357, Length=209, Percent_Identity=26.7942583732057, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4504503, Length=195, Percent_Identity=31.2820512820513, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1786812, Length=186, Percent_Identity=31.7204301075269, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786701, Length=227, Percent_Identity=29.0748898678414, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787335, Length=189, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790717, Length=186, Percent_Identity=26.3440860215054, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788459, Length=198, Percent_Identity=28.2828282828283, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082100, Length=183, Percent_Identity=29.5081967213115, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367175, Length=190, Percent_Identity=30.5263157894737, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=237, Percent_Identity=30.379746835443, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994600, Length=215, Percent_Identity=30.6976744186047, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115534660, Length=192, Percent_Identity=30.7291666666667, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=210, Percent_Identity=30.952380952381, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=212, Percent_Identity=30.188679245283, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=188, Percent_Identity=30.3191489361702, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17557780, Length=196, Percent_Identity=29.0816326530612, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=203, Percent_Identity=28.5714285714286, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24651139, Length=282, Percent_Identity=27.6595744680851, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571526, Length=189, Percent_Identity=32.8042328042328, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI23397609, Length=226, Percent_Identity=31.4159292035398, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21357041, Length=188, Percent_Identity=31.3829787234043, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24644339, Length=250, Percent_Identity=31.6, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281364892, Length=190, Percent_Identity=30.5263157894737, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24643142, Length=186, Percent_Identity=32.258064516129, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 31383; Mature: 31383

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVVLVTGASSGFGLLTVQALARAGHTVYASMRERAGRNAPRAAAVAAYAQEHGVDLRS
CCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEE
LELDVGDDASVDAAIERVITDNGRLDVVVHNAGHMVFGPAEAFTPEQLAQLYDVNVVSTQ
EEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEECCHHCCCHHHHHHHHCCCCHHHH
RVNRAALPHLRRQGRGLLVWVSSSSARGGTPPFLAPYFAAKAAMDSLAVSYAAELARWGI
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETSIVVPGAFTRGTNHFLHAGKPADTAVQAAYDSGPYAGVADQALTGLAALEPADADAGA
CEEEEECCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHH
VATAIAELVAAPAGKRPYRVFVDPSQDGAEEVFRVGDRIRREMFRNIGLADLLAPRVDG
HHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
>Mature Secondary Structure
MKEVVLVTGASSGFGLLTVQALARAGHTVYASMRERAGRNAPRAAAVAAYAQEHGVDLRS
CCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEE
LELDVGDDASVDAAIERVITDNGRLDVVVHNAGHMVFGPAEAFTPEQLAQLYDVNVVSTQ
EEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEECCHHCCCHHHHHHHHCCCCHHHH
RVNRAALPHLRRQGRGLLVWVSSSSARGGTPPFLAPYFAAKAAMDSLAVSYAAELARWGI
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETSIVVPGAFTRGTNHFLHAGKPADTAVQAAYDSGPYAGVADQALTGLAALEPADADAGA
CEEEEECCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHH
VATAIAELVAAPAGKRPYRVFVDPSQDGAEEVFRVGDRIRREMFRNIGLADLLAPRVDG
HHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 8396117 [H]