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The map label for this gene is metG [H]

Identifier: 116690457

GI number: 116690457

Start: 2705956

End: 2708112

Strand: Direct

Name: metG [H]

Synonym: Bcen2424_2437

Alternate gene names: 116690457

Gene position: 2705956-2708112 (Clockwise)

Preceding gene: 116690456

Following gene: 116690460

Centisome position: 77.67

GC content: 65.0

Gene sequence:

>2157_bases
ATGTCCGCATCCGACCTCACTTCCGTGCAGGCTGCGGCGCCGCAAGGCAGCCGCCAGATCCTCGTTACGTCCGCACTGCC
CTATGCCAACGGGCAGATCCACATCGGCCACCTGGTCGAGTACATCCAGACCGACATCTGGGTGCGGACGCTGCGAATGC
ATGGCCATGAGGTCTACTACATCGGCGCCGACGATACGCACGGCACGCCGATCATGCTGCGCGCGGAGAAGGAAGGCCTG
ACGCCGAAGCAGCTGATCGACCGCGTGTGGACCGAGCACAAGCGCGACTTCGACAGCTTCGGCGTGTCGTTCGACAATTT
CTACACGACCGATTCGGAAGAAAACCGCGTGCTGAGCGAGAACATCTATCTCGCGCTGCAGGAAGCCGGCCTGATCGCCG
AACGCGAAATCGAGCAAGCCTACGATCCGGTCAAGGAAATGTTCCTGCCGGACCGCTTCATCAAGGGCGAGTGTCCGAAG
TGTCACGCGAAGGACCAGTACGGCGACAGCTGCGAGGTGTGCGGCTCGACCTACCTGCCGACCGAACTGCTGAATCCGTA
TTCGGTCGTGTCGGGCGCGACGCCGGTGCGCAAGACCTCGAAGCACTACTTCTTCCGCCTGTCCGACCCGCGCTGCGAAT
CGTTCCTGCGCGAGTGGGTGAGCGGCCTCGCGCAGCCCGAAGCGACCAACAAGATGCGCGAATGGCTCGGCGACGCCGGC
GAATCCAAGCTCGCCGACTGGGACATCTCGCGCGACGCGCCGTACTTCGGCTTCGAGATCCCCGGTGCGCCCGGCAAGTA
TTTCTACGTGTGGCTCGACGCGCCGGTCGGCTACTACGCGAGCTTCAAGAACCTGTGCGAGCGCAACGGCATCGATTTCG
ATGCGTGGATCCGCCCGGGCTCGAAGGCCGAGCAGTACCACTTCATCGGCAAGGACATTCTGTACTTCCACACGCTGTTC
TGGCCCGCGATGCTCGAATTCTCGGGCCACCGCACGCCGACCAACGTGTTCGCGCACGGCTTCCTGACGGTCGACGGCGC
GAAGATGTCGAAGTCGCGCGGCACGTTCATCACCGCGCAGAGCTACATCGACACCGGCCTGAACCCCGAGTGGCTGCGCT
ACTACTTCGCCGCGAAGCTGAACGCGACGATGGAAGACATCGACCTGAACCTCGACGACTTCCAGGCGCGCGTGAACAGC
GACCTCGTCGGCAAGTACGTGAACATCGCGAGCCGCGCGGCCGGCTTCCTGATCAAGCGTTTCGACGGTCGCGTGCAGGA
CAGCGCGATGAATCATCCGCTCGTCGCAAAGCTGCGCGATGCGATTCCACAGATCGCCGCGAGCTACGAAGCACGCGAAT
ACGGCCGCGCGCTGCGCCACACGATGGAGCTCGCGGACGAAGTGAACGCGTACGTCGACGGCGCGAAGCCGTGGGATCTC
GCGAAGGACCCGGCCAACGCGGTCGCGCTGCACGAAACCTGCAGCGTGAGCCTCGAGGCCTTCCGCCTGCTGTCGCTCGC
GCTGAAGCCGGTGATGCCGCGCGTGGCCGTTGCGGTCGAGGCGTTCTTCGGGATCGCGCCGCTCGCGTGGGCCGACGCCG
CGAAGCCGCTGTCGTCGGCGCAACCGATCAAGGCGTACCAGCACCTGATGACGCGCGTCGATGCGAAGCAGATCGAAGCG
CTGCTCGCCGCGAACCGCGATTCGCTGCAGGCCGAGGCAGCCGGCGCGGCCGCCGCGGGCGCCAATGCCGCGAAGGATGC
GAAGAGCAACGCGAAGGCGAGCGCGAAGCCGGCGGCCGTGAACGGCGCCGACGACGCACCGATCTCGATCGACGATTTCG
CGAAGATCGATCTGCGCATCGCGAAGATCGTCGCGTGTCAGGCGGTCGAAGGCTCGGACAAGCTGCTGCAGCTCACGCTC
GACGTCGGCGAGGAAAAGACCCGCAACGTGTTCTCGGGCATCAAGTCCGCGTATCAGCCCGAGCAGCTCGTCGGCAAGCT
GACGGTGATGGTCGCGAACCTCGCGCCGCGCAAGATGAAGTTCGGCCTGTCCGAAGGGATGGTGCTCGCCGCATCGGCCA
CCGACGAGAAGGCGGAACCGGGCCTCTACATCCTCGAACCGCACAGCGGCGCGAAGCCCGGCATGCGCGTGAAGTAA

Upstream 100 bases:

>100_bases
GCAGCCGGCCGGTCCACGGCACCCGCCCGCCGCGCGAGCCCGCGCCCCTGCTAGAATCGCAGTTTCCCGTCGTTTTTCCC
GCTGTTCTTCTCCAGACCCT

Downstream 100 bases:

>100_bases
GCGCAACGCGCAGCTGCTGCTTGCGCAAAAAAGCCCCGCACGACTTGCATCGTGCGGGGCTTTTTTATCGCTGCCCGGTA
TGCCGCGCCCTTCGTCAGCG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MSASDLTSVQAAAPQGSRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPIMLRAEKEGL
TPKQLIDRVWTEHKRDFDSFGVSFDNFYTTDSEENRVLSENIYLALQEAGLIAEREIEQAYDPVKEMFLPDRFIKGECPK
CHAKDQYGDSCEVCGSTYLPTELLNPYSVVSGATPVRKTSKHYFFRLSDPRCESFLREWVSGLAQPEATNKMREWLGDAG
ESKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCERNGIDFDAWIRPGSKAEQYHFIGKDILYFHTLF
WPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNS
DLVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLRDAIPQIAASYEAREYGRALRHTMELADEVNAYVDGAKPWDL
AKDPANAVALHETCSVSLEAFRLLSLALKPVMPRVAVAVEAFFGIAPLAWADAAKPLSSAQPIKAYQHLMTRVDAKQIEA
LLAANRDSLQAEAAGAAAAGANAAKDAKSNAKASAKPAAVNGADDAPISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTL
DVGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRVK

Sequences:

>Translated_718_residues
MSASDLTSVQAAAPQGSRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPIMLRAEKEGL
TPKQLIDRVWTEHKRDFDSFGVSFDNFYTTDSEENRVLSENIYLALQEAGLIAEREIEQAYDPVKEMFLPDRFIKGECPK
CHAKDQYGDSCEVCGSTYLPTELLNPYSVVSGATPVRKTSKHYFFRLSDPRCESFLREWVSGLAQPEATNKMREWLGDAG
ESKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCERNGIDFDAWIRPGSKAEQYHFIGKDILYFHTLF
WPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNS
DLVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLRDAIPQIAASYEAREYGRALRHTMELADEVNAYVDGAKPWDL
AKDPANAVALHETCSVSLEAFRLLSLALKPVMPRVAVAVEAFFGIAPLAWADAAKPLSSAQPIKAYQHLMTRVDAKQIEA
LLAANRDSLQAEAAGAAAAGANAAKDAKSNAKASAKPAAVNGADDAPISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTL
DVGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRVK
>Mature_717_residues
SASDLTSVQAAAPQGSRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPIMLRAEKEGLT
PKQLIDRVWTEHKRDFDSFGVSFDNFYTTDSEENRVLSENIYLALQEAGLIAEREIEQAYDPVKEMFLPDRFIKGECPKC
HAKDQYGDSCEVCGSTYLPTELLNPYSVVSGATPVRKTSKHYFFRLSDPRCESFLREWVSGLAQPEATNKMREWLGDAGE
SKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCERNGIDFDAWIRPGSKAEQYHFIGKDILYFHTLFW
PAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSD
LVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLRDAIPQIAASYEAREYGRALRHTMELADEVNAYVDGAKPWDLA
KDPANAVALHETCSVSLEAFRLLSLALKPVMPRVAVAVEAFFGIAPLAWADAAKPLSSAQPIKAYQHLMTRVDAKQIEAL
LAANRDSLQAEAAGAAAAGANAAKDAKSNAKASAKPAAVNGADDAPISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLD
VGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=608, Percent_Identity=26.9736842105263, Blast_Score=219, Evalue=7e-57,
Organism=Homo sapiens, GI24308436, Length=538, Percent_Identity=26.9516728624535, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI215490011, Length=115, Percent_Identity=34.7826086956522, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI215490009, Length=115, Percent_Identity=34.7826086956522, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI45006986, Length=115, Percent_Identity=34.7826086956522, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI4507947, Length=139, Percent_Identity=33.8129496402878, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1788432, Length=714, Percent_Identity=52.8011204481793, Blast_Score=740, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=677, Percent_Identity=25.9970457902511, Blast_Score=225, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI212645096, Length=519, Percent_Identity=24.8554913294798, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71992781, Length=344, Percent_Identity=25.2906976744186, Blast_Score=98, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321704, Length=564, Percent_Identity=27.8368794326241, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321610, Length=544, Percent_Identity=25.3676470588235, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24655311, Length=528, Percent_Identity=29.9242424242424, Blast_Score=227, Evalue=2e-59,
Organism=Drosophila melanogaster, GI21358483, Length=519, Percent_Identity=27.5529865125241, Blast_Score=137, Evalue=3e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 79228; Mature: 79096

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASDLTSVQAAAPQGSRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYY
CCCCHHCHHHHCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPIMLRAEKEGLTPKQLIDRVWTEHKRDFDSFGVSFDNFYTTDSEENRVLSE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCEECCCCCCCCEECC
NIYLALQEAGLIAEREIEQAYDPVKEMFLPDRFIKGECPKCHAKDQYGDSCEVCGSTYLP
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
TELLNPYSVVSGATPVRKTSKHYFFRLSDPRCESFLREWVSGLAQPEATNKMREWLGDAG
HHHCCCHHHHCCCCCCHHCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
ESKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCERNGIDFDAWIRPG
CCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHEECCC
SKAEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEH
SYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSDLVGKYVNIASRAAGFLIKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FDGRVQDSAMNHPLVAKLRDAIPQIAASYEAREYGRALRHTMELADEVNAYVDGAKPWDL
HCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AKDPANAVALHETCSVSLEAFRLLSLALKPVMPRVAVAVEAFFGIAPLAWADAAKPLSSA
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
QPIKAYQHLMTRVDAKQIEALLAANRDSLQAEAAGAAAAGANAAKDAKSNAKASAKPAAV
CHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCHHCCCCHHHHHHCCCCCCCCCCCC
NGADDAPISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYQP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCH
EQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRVK
HHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SASDLTSVQAAAPQGSRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYY
CCCHHCHHHHCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPIMLRAEKEGLTPKQLIDRVWTEHKRDFDSFGVSFDNFYTTDSEENRVLSE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCEECCCCCCCCEECC
NIYLALQEAGLIAEREIEQAYDPVKEMFLPDRFIKGECPKCHAKDQYGDSCEVCGSTYLP
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
TELLNPYSVVSGATPVRKTSKHYFFRLSDPRCESFLREWVSGLAQPEATNKMREWLGDAG
HHHCCCHHHHCCCCCCHHCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
ESKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCERNGIDFDAWIRPG
CCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHEECCC
SKAEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEH
SYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSDLVGKYVNIASRAAGFLIKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FDGRVQDSAMNHPLVAKLRDAIPQIAASYEAREYGRALRHTMELADEVNAYVDGAKPWDL
HCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AKDPANAVALHETCSVSLEAFRLLSLALKPVMPRVAVAVEAFFGIAPLAWADAAKPLSSA
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
QPIKAYQHLMTRVDAKQIEALLAANRDSLQAEAAGAAAAGANAAKDAKSNAKASAKPAAV
CHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCHHCCCCHHHHHHCCCCCCCCCCCC
NGADDAPISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYQP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCH
EQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRVK
HHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA