Click here to switch to the map view.

The map label for this gene is rfaQ [H]

Identifier: 116690332

GI number: 116690332

Start: 2566390

End: 2567568

Strand: Reverse

Name: rfaQ [H]

Synonym: Bcen2424_2311

Alternate gene names: 116690332

Gene position: 2567568-2566390 (Counterclockwise)

Preceding gene: 116690333

Following gene: 116690328

Centisome position: 73.7

GC content: 70.48

Gene sequence:

>1179_bases
GTGGCCCTGTTTTCTTCCGCCCGTCCGCCCAGAACCATCCTCGTTGCCGCGCCGCGCCGTATCGGCGACGTACTGTTGAC
GACCCCGCTCGTACGGTCCCTGAAGGCCCGCTGGCCGGACGCGCAGATCGACATGCTGGTGTTTCGCGGCACCGAGGGCG
TGCTCGAGCACAATCCGGACGTGCGGCGCGTGATCGTGGTCGCGCAGCGTGCGGGATTGGGCGAGCGGCTGCGCGACGCG
CTGTCGATGTGGCGCCGCTACGATCTCGCATGCGCGGCGCTGAGTTCCGACCGGCCGCGTTTCTATAGCTGGTTCGCCGG
CCGCAAACGGGTCGGCCTCGTCGACCCGAATCGCGTCACGTGGCTGACGCGGATGATGCTGAACGGGATCGCGATCAATC
ACCACGAATCTGCCCATACGGTCGTCAGTACGCTGGCACTCGCGCTGGTGATCGGCATCGAGCCGGTGTCCGAGGTCGTC
GCGCCCGGCATCGGCGATGATCCGGCGCGGCGCGCGCGTTTCGACGCGTGGCTCGCCGAGTCGCCGGCGATCCGCGCCGG
CAAGCCGCTGGTCGTGCTGCATCCGTACCCGATGTTCCGTTACAAGCAATGGCGGCTCGACGGCTGGGTCGAAATGATCG
GCTGGCTGCGCGAGCAGGGCTTCGCGATCGCGCTGTCGGGCGGCCCGGCCGACAGCGAGCGCGAGTATGCGGAGCAGGTC
GCGACGGAGGCGGGCGGCGACGTGCTGAACCTGGTCGGCCGCCTGACGTTCGGCGAGAGCGCGGAGCTCGTGCGGCGCGC
GCGGCTCTTCATCGGGCCCGACACCGGCGCGACGCACGTCGCGGCCGCGACGGGCACCGACACGATTGCACTGTTCGGGC
CGTCCGACCCCGTGCGTTGGGGGCCGTGGCCGCAGCACTGGCCTGCGACCGAGAATCCGTGGGCGCTGCGCGGCTCGGGG
CGGCACGGAAACGTGTGGCTGTTGCAGGGGGACGGAGATTGCGTGCCGTGCCGCCACGAAGGCTGCGAGCGTCACGTCGA
CAGTCGCAGCGACTGCCTCGTCCATCTGGATGCGCAGCGCGTGAAGGCCGCGGCAGCCGAGATGTTGGGGCTGAATCCGC
CGGGGCCGGCCGACGTCATCGTCGACACGTCGCGGCTGCGTCGTGCCGGGGCCGACTGA

Upstream 100 bases:

>100_bases
GCCGCATCGTGGCCGCTTCCATCGCCGGCGCGCGACGCCGCGCCGGCTGCTGCAAAATCCTGTACATTGCCAGCCTTGTT
CAGCCAGCCAGACGATCGCC

Downstream 100 bases:

>100_bases
CGCGCGCCTTTCTTCGTCAGCAGGAGGCTGTTTCGCGGAGCGCCGCGCGGCTCTCCGGCATCGACGGCGGATGTCCGCCG
CCGCCGAACCGGGTGGGGGC

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MALFSSARPPRTILVAAPRRIGDVLLTTPLVRSLKARWPDAQIDMLVFRGTEGVLEHNPDVRRVIVVAQRAGLGERLRDA
LSMWRRYDLACAALSSDRPRFYSWFAGRKRVGLVDPNRVTWLTRMMLNGIAINHHESAHTVVSTLALALVIGIEPVSEVV
APGIGDDPARRARFDAWLAESPAIRAGKPLVVLHPYPMFRYKQWRLDGWVEMIGWLREQGFAIALSGGPADSEREYAEQV
ATEAGGDVLNLVGRLTFGESAELVRRARLFIGPDTGATHVAAATGTDTIALFGPSDPVRWGPWPQHWPATENPWALRGSG
RHGNVWLLQGDGDCVPCRHEGCERHVDSRSDCLVHLDAQRVKAAAAEMLGLNPPGPADVIVDTSRLRRAGAD

Sequences:

>Translated_392_residues
MALFSSARPPRTILVAAPRRIGDVLLTTPLVRSLKARWPDAQIDMLVFRGTEGVLEHNPDVRRVIVVAQRAGLGERLRDA
LSMWRRYDLACAALSSDRPRFYSWFAGRKRVGLVDPNRVTWLTRMMLNGIAINHHESAHTVVSTLALALVIGIEPVSEVV
APGIGDDPARRARFDAWLAESPAIRAGKPLVVLHPYPMFRYKQWRLDGWVEMIGWLREQGFAIALSGGPADSEREYAEQV
ATEAGGDVLNLVGRLTFGESAELVRRARLFIGPDTGATHVAAATGTDTIALFGPSDPVRWGPWPQHWPATENPWALRGSG
RHGNVWLLQGDGDCVPCRHEGCERHVDSRSDCLVHLDAQRVKAAAAEMLGLNPPGPADVIVDTSRLRRAGAD
>Mature_391_residues
ALFSSARPPRTILVAAPRRIGDVLLTTPLVRSLKARWPDAQIDMLVFRGTEGVLEHNPDVRRVIVVAQRAGLGERLRDAL
SMWRRYDLACAALSSDRPRFYSWFAGRKRVGLVDPNRVTWLTRMMLNGIAINHHESAHTVVSTLALALVIGIEPVSEVVA
PGIGDDPARRARFDAWLAESPAIRAGKPLVVLHPYPMFRYKQWRLDGWVEMIGWLREQGFAIALSGGPADSEREYAEQVA
TEAGGDVLNLVGRLTFGESAELVRRARLFIGPDTGATHVAAATGTDTIALFGPSDPVRWGPWPQHWPATENPWALRGSGR
HGNVWLLQGDGDCVPCRHEGCERHVDSRSDCLVHLDAQRVKAAAAEMLGLNPPGPADVIVDTSRLRRAGAD

Specific function: Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790062, Length=367, Percent_Identity=26.158038147139, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790050, Length=302, Percent_Identity=25.8278145695364, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011916 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 43084; Mature: 42953

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFSSARPPRTILVAAPRRIGDVLLTTPLVRSLKARWPDAQIDMLVFRGTEGVLEHNPD
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHCCCC
VRRVIVVAQRAGLGERLRDALSMWRRYDLACAALSSDRPRFYSWFAGRKRVGLVDPNRVT
CEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEECCCCCCHHH
WLTRMMLNGIAINHHESAHTVVSTLALALVIGIEPVSEVVAPGIGDDPARRARFDAWLAE
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCC
SPAIRAGKPLVVLHPYPMFRYKQWRLDGWVEMIGWLREQGFAIALSGGPADSEREYAEQV
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
ATEAGGDVLNLVGRLTFGESAELVRRARLFIGPDTGATHVAAATGTDTIALFGPSDPVRW
HHHCCCHHHHHHHHCCCCCHHHHHHHHHEEECCCCCCCEEEEECCCCEEEEECCCCCCCC
GPWPQHWPATENPWALRGSGRHGNVWLLQGDGDCVPCRHEGCERHVDSRSDCLVHLDAQR
CCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCEECCCHHHHHHHCCCCCCEEEEECHHH
VKAAAAEMLGLNPPGPADVIVDTSRLRRAGAD
HHHHHHHHHCCCCCCCCCEEEEHHHHHHCCCC
>Mature Secondary Structure 
ALFSSARPPRTILVAAPRRIGDVLLTTPLVRSLKARWPDAQIDMLVFRGTEGVLEHNPD
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHCCCC
VRRVIVVAQRAGLGERLRDALSMWRRYDLACAALSSDRPRFYSWFAGRKRVGLVDPNRVT
CEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEECCCCCCHHH
WLTRMMLNGIAINHHESAHTVVSTLALALVIGIEPVSEVVAPGIGDDPARRARFDAWLAE
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCC
SPAIRAGKPLVVLHPYPMFRYKQWRLDGWVEMIGWLREQGFAIALSGGPADSEREYAEQV
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
ATEAGGDVLNLVGRLTFGESAELVRRARLFIGPDTGATHVAAATGTDTIALFGPSDPVRW
HHHCCCHHHHHHHHCCCCCHHHHHHHHHEEECCCCCCCEEEEECCCCEEEEECCCCCCCC
GPWPQHWPATENPWALRGSGRHGNVWLLQGDGDCVPCRHEGCERHVDSRSDCLVHLDAQR
CCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCEECCCHHHHHHHCCCCCCEEEEECHHH
VKAAAAEMLGLNPPGPADVIVDTSRLRRAGAD
HHHHHHHHHCCCCCCCCCEEEEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Transferring other glycosyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732225; 1447141; 8041620; 9278503 [H]