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The map label for this gene is alaXM [H]

Identifier: 116690299

GI number: 116690299

Start: 2532128

End: 2532859

Strand: Reverse

Name: alaXM [H]

Synonym: Bcen2424_2278

Alternate gene names: 116690299

Gene position: 2532859-2532128 (Counterclockwise)

Preceding gene: 116690300

Following gene: 116690298

Centisome position: 72.7

GC content: 68.85

Gene sequence:

>732_bases
ATGACGACACAAGCGCTGTTTCGCGAAGACGCGTACCTCACGCAATGCGAGGCGATCGTCCAGGCCATCGGCGAAGACGG
CATCCGGCTCGACCGCACCGTGTTCTATCCGCTCGGCGGCGGCCAGGCCGGCGACACCGGCACGCTGACGCTGCCCGACG
GCGGCACGATCGCGATCGCCGACACGCGCAAGGCGAAGTTCGACGGCGCGACGCCCGACGACGCCGTCCACGTGCCAGCG
TCGGGCCAGGAGGCGAGCGTCGCGACGCTCGTCCCCGGCACGCGCGTGGTTGCTGAGATCGACTGGCTGCGCCGCTACCG
GCACATGCGTCTGCACACGGCCGCGCACCTGATGTGCGCGGTGTTGCCGTACGCGGTCGACGGTTGCAGCGTGACGGCCG
ACTACGTGCGGCTCGATTTCGCGACTGCCGACGCGATCGACCGCGACGACGTCGAGCGGCGCCTCGCGGGCTTCGTCGTC
GGCGCGCATCCGGTCACGACCGAATGGATTACCGACGACGAGATGGCCGAGCGTCCCGAACTCGTGCGCACGATGAGCGT
GAAGCCGCCGATGGGCCTCGGCCGCGTGCGGCTGCTGCGCATCGAAAACGTCGACCTGCAGCCGTGCGGCGGCACGCATG
TGCGCAACACGTCCGAAATCGGCGGGTTGCGGGTCGCGAAACTGGAAAAGAAGAGTGCGCGCACACGGCGCCTCGTACTG
GAGTTTGCATGA

Upstream 100 bases:

>100_bases
ACCGGTACGCCGCTGTCCGCGCGCGGGACGATCTGCGTCTGGGCGCACGAGCGGCCCGGCGCGACACTGGCCGTTTCTCC
ATCGATACGAGGCTTGCCCG

Downstream 100 bases:

>100_bases
CGGTCGATACCGGGAAAGCAGGCGTGGCGGCACTCGATCCGCAGGCACGGGAGATTCTCGATTTCTGGTTCGGCGAGCCG
GGCTCGGCCGAATTCGGGCA

Product: threonyl/alanyl tRNA synthetase, SAD

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTTQALFREDAYLTQCEAIVQAIGEDGIRLDRTVFYPLGGGQAGDTGTLTLPDGGTIAIADTRKAKFDGATPDDAVHVPA
SGQEASVATLVPGTRVVAEIDWLRRYRHMRLHTAAHLMCAVLPYAVDGCSVTADYVRLDFATADAIDRDDVERRLAGFVV
GAHPVTTEWITDDEMAERPELVRTMSVKPPMGLGRVRLLRIENVDLQPCGGTHVRNTSEIGGLRVAKLEKKSARTRRLVL
EFA

Sequences:

>Translated_243_residues
MTTQALFREDAYLTQCEAIVQAIGEDGIRLDRTVFYPLGGGQAGDTGTLTLPDGGTIAIADTRKAKFDGATPDDAVHVPA
SGQEASVATLVPGTRVVAEIDWLRRYRHMRLHTAAHLMCAVLPYAVDGCSVTADYVRLDFATADAIDRDDVERRLAGFVV
GAHPVTTEWITDDEMAERPELVRTMSVKPPMGLGRVRLLRIENVDLQPCGGTHVRNTSEIGGLRVAKLEKKSARTRRLVL
EFA
>Mature_242_residues
TTQALFREDAYLTQCEAIVQAIGEDGIRLDRTVFYPLGGGQAGDTGTLTLPDGGTIAIADTRKAKFDGATPDDAVHVPAS
GQEASVATLVPGTRVVAEIDWLRRYRHMRLHTAAHLMCAVLPYAVDGCSVTADYVRLDFATADAIDRDDVERRLAGFVVG
AHPVTTEWITDDEMAERPELVRTMSVKPPMGLGRVRLLRIENVDLQPCGGTHVRNTSEIGGLRVAKLEKKSARTRRLVLE
FA

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789048, Length=220, Percent_Identity=29.5454545454545, Blast_Score=64, Evalue=8e-12,
Organism=Drosophila melanogaster, GI18543305, Length=211, Percent_Identity=27.9620853080569, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 26485; Mature: 26353

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQALFREDAYLTQCEAIVQAIGEDGIRLDRTVFYPLGGGQAGDTGTLTLPDGGTIAIA
CCCHHHHHCCHHHHHHHHHHHHHCCCCCEECEEEEEECCCCCCCCCCEEECCCCCEEEEE
DTRKAKFDGATPDDAVHVPASGQEASVATLVPGTRVVAEIDWLRRYRHMRLHTAAHLMCA
CCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLPYAVDGCSVTADYVRLDFATADAIDRDDVERRLAGFVVGAHPVTTEWITDDEMAERPE
HHHHHCCCCCEEEEHEEEEEHHCCCCCHHHHHHHHHHEEECCCCCEEEECCCHHHHHCHH
LVRTMSVKPPMGLGRVRLLRIENVDLQPCGGTHVRNTSEIGGLRVAKLEKKSARTRRLVL
HHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHCCEEEEHHHHHHHHHEEEEE
EFA
EEC
>Mature Secondary Structure 
TTQALFREDAYLTQCEAIVQAIGEDGIRLDRTVFYPLGGGQAGDTGTLTLPDGGTIAIA
CCHHHHHCCHHHHHHHHHHHHHCCCCCEECEEEEEECCCCCCCCCCEEECCCCCEEEEE
DTRKAKFDGATPDDAVHVPASGQEASVATLVPGTRVVAEIDWLRRYRHMRLHTAAHLMCA
CCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLPYAVDGCSVTADYVRLDFATADAIDRDDVERRLAGFVVGAHPVTTEWITDDEMAERPE
HHHHHCCCCCEEEEHEEEEEHHCCCCCHHHHHHHHHHEEECCCCCEEEECCCHHHHHCHH
LVRTMSVKPPMGLGRVRLLRIENVDLQPCGGTHVRNTSEIGGLRVAKLEKKSARTRRLVL
HHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHCCEEEEHHHHHHHHHEEEEE
EFA
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]