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The map label for this gene is ilvI [H]

Identifier: 116690286

GI number: 116690286

Start: 2519737

End: 2521500

Strand: Reverse

Name: ilvI [H]

Synonym: Bcen2424_2265

Alternate gene names: 116690286

Gene position: 2521500-2519737 (Counterclockwise)

Preceding gene: 116690298

Following gene: 116690285

Centisome position: 72.38

GC content: 62.7

Gene sequence:

>1764_bases
ATGAACATGCCCAGCGCGGAATTCTCCACGTCGGAACCCCTTTCCCCTCCCGATAGCGACTCCATCGGCGGTACCGTGCT
CATGAAGGCACTGGCCGACGAAAACGTCGAATTCATCTGGGGCTACCCCGGCGGCTCGGTTCTCTACATCTACGACGAGC
TGTACAAGCAGGACAAGATTCAGCACGTGCTGGTGCGCCATGAACAGGCGGCCGTGCACGCAGCCGATGCGTATGCGCGC
TCCACCGGCAATGTCGGCGTCTGCCTCGTGACGTCGGGCCCCGGTGTCACCAACGCGGTGACCGGCATCGCAACGGCTTA
CATGGATTCGATCCCGATGGTCGTGATCAGCGGCCAGGTGCCGACTGCCGCGATCGGCCAGGATGCGTTCCAGGAATGCG
ACACCGTCGGCATCACGCGCCCGTGCGTGAAGCACAACTTCCTCGTGAAGGACGTGCGCGACCTCGCGGCAACCGTCAAG
AAGGCGTTCTATATCGCCCGCACCGGCCGTCCGGGCCCGGTGCTGATCGACATCCCGAAGGACATCTCGAAGACGCCGTG
CCAGTACGAGCCGATCAAGAGCGTGTCGCTGCGTTCGTACAACCCCGTCACGAAAGGCCATTCGGGCCAGATCCGCAAGG
CCGTGTCGCTGCTGCTGACCGCGAAGCGTCCGTACATCTACACGGGTGGCGGCATCATCCTCGCCGACGCGTCGCGTGAA
CTGAACCAGTTCGCGGACCTGCTCGGCTACCCGGTCACGAACACGCTGATGGGCCTCGGCGGCTATCGCGCGTCGGACAA
GAAATTTCTCGGCATGCTCGGCATGCACGGCACCTACGAAGCGAACATGGCGATGCAGCACTGCGACGTGCTGATCGCGA
TCGGCGCCCGCTTCGACGACCGCGTGATCGGCGACCCGGCGCACTTCGCGTCGCGCCCGCGCAAGATCATTCACATCGAC
ATCGACCCGTCGTCGATTTCGAAGCGCGTGAAGGTCGACATCCCGATCGTCGGCGACGTGAAGGAAGTGCTGAAGGAGCT
GATCGAGCAACTGCAGACGGCCGAGCATGGTCCCGACACCGAGGCGCTCGCGCAATGGTGGAAGGACATCGAAGGCTGGC
GCGCGAAGGACTGCCTGAAGTTCGACCGCGAAAGCGAGATCATCAAGCCGCAGTACGTGGTCGAAAAGGCGTGGGAGCTG
ACGGACGGCAATGCGTTCGTGTGCTCGGACGTCGGCCAGCACCAGATGTGGGCCGCGCAGTTCTACCGTTTCAACAAGCC
GCGTCGCTGGATCAACTCCGGCGGCCTCGGCACGATGGGCTTCGGCCTGCCGGCGGCGATGGGCGTGAAGATGGCCCATC
CGGACGACGACGTGCTGTGCATCACGGGCGAAGGCTCGATCCAGATGTGCATCCAGGAACTGTCGACCTGCCTGCAGTAC
GACACGCCCGTGAAGATCATTTCGCTGAACAACCGCTACCTCGGCATGGTCCGCCAGTGGCAGCAGATCGAATACAGCAA
GCGCTATTCGCATTCGTACATGGATGCGCTGCCCGATTTCGTGAAGCTCGCCGAAGCGTACGGCCATGTCGGCATGCGGA
TCGAAAAGACTTCGGATGTGGAGCCGGCGCTGAAGGAAGCGCTGCGCCTGAAGGACCGCACCGTGTTTCTCGACTTCCAG
ACCGATCCGACCGAAAACGTCTGGCCGATGGTACAGGCCGGCAAGGGCATCACCGAGATGCTGCTCGGATCGGAAGATCT
GTAA

Upstream 100 bases:

>100_bases
AGAAGCCCGCCCTTCAAACTGGACGCGCCGCTTGAACCCGAGCCACAAGCCCGGCAGAGAGCACCCGATCAATTTTTTGC
CGAAAATTCGAAAGGTCAAA

Downstream 100 bases:

>100_bases
CGGCGCGACGCGCGTCTGGCCCGATGCGGCCGCCTTCACGAAGGGTGGTGCGGCACGCGGCGGCGCGCGGCGCGAGTGAA
TCGACACGGACACATTCTGG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNMPSAEFSTSEPLSPPDSDSIGGTVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYAR
STGNVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVK
KAFYIARTGRPGPVLIDIPKDISKTPCQYEPIKSVSLRSYNPVTKGHSGQIRKAVSLLLTAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTEALAQWWKDIEGWRAKDCLKFDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTSDVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL

Sequences:

>Translated_587_residues
MNMPSAEFSTSEPLSPPDSDSIGGTVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYAR
STGNVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVK
KAFYIARTGRPGPVLIDIPKDISKTPCQYEPIKSVSLRSYNPVTKGHSGQIRKAVSLLLTAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTEALAQWWKDIEGWRAKDCLKFDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTSDVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL
>Mature_587_residues
MNMPSAEFSTSEPLSPPDSDSIGGTVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYAR
STGNVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVK
KAFYIARTGRPGPVLIDIPKDISKTPCQYEPIKSVSLRSYNPVTKGHSGQIRKAVSLLLTAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTEALAQWWKDIEGWRAKDCLKFDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTSDVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=583, Percent_Identity=25.0428816466552, Blast_Score=171, Evalue=3e-42,
Organism=Homo sapiens, GI21361361, Length=568, Percent_Identity=27.112676056338, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI87081685, Length=571, Percent_Identity=57.7933450087566, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=39.8214285714286, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=34.0823970037453, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1787096, Length=563, Percent_Identity=28.2415630550622, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=24.187725631769, Blast_Score=145, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=26.1818181818182, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=550, Percent_Identity=26.1818181818182, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=510, Percent_Identity=26.6666666666667, Blast_Score=143, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=592, Percent_Identity=42.3986486486487, Blast_Score=457, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=24.2105263157895, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=533, Percent_Identity=22.3264540337711, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321524, Length=528, Percent_Identity=21.780303030303, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=500, Percent_Identity=22.2, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=26.0176991150442, Blast_Score=181, Evalue=2e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64892; Mature: 64892

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMPSAEFSTSEPLSPPDSDSIGGTVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHVLVRHEQAAVHAADAYARSTGNVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DISKTPCQYEPIKSVSLRSYNPVTKGHSGQIRKAVSLLLTAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDT
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
EALAQWWKDIEGWRAKDCLKFDRESEIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHCC
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTSDV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MNMPSAEFSTSEPLSPPDSDSIGGTVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHVLVRHEQAAVHAADAYARSTGNVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DISKTPCQYEPIKSVSLRSYNPVTKGHSGQIRKAVSLLLTAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDT
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
EALAQWWKDIEGWRAKDCLKFDRESEIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHCC
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIEKTSDV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]