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The map label for this gene is yfcG [H]

Identifier: 116690249

GI number: 116690249

Start: 2483650

End: 2484342

Strand: Direct

Name: yfcG [H]

Synonym: Bcen2424_2228

Alternate gene names: 116690249

Gene position: 2483650-2484342 (Clockwise)

Preceding gene: 116690248

Following gene: 116690277

Centisome position: 71.29

GC content: 63.64

Gene sequence:

>693_bases
ATGATCGACGTCTATAGCTGGGCGACCCCGAACGGCCACAAGGTACACATCATGCTCGAGGAAACCGGCCTCGCGTATCG
TGCGCATCCGGTCGATATCGGCGCCGGCGACCAGTTCGAGCCCGAGTTCCTGAAGATCAGCCCGAACAACAAGATTCCGG
CAATCGTCGATCCGGACGGCCCCGGCGGCCAATCGATCTCGCTGTTCGAATCGGGCGCGATCCTGATTTACCTCGCGGAG
AAGACCGGCAAGTTCCTGCCGACCGATCCGGCCGCGCGCTATGCGACGCTCGAGTGGCTGATGTTCCAGATGGGCGGTGT
CGGCCCGATGCTCGGGCAGGCGCACCACTTCCGGCTGTATGCGCCGGAGAAGATCGAGTATGCGGTCAACCGCTATACGA
ACGAAGCGAAGCGCCTGTACAACGTGATGGAAAAACGCCTCGGCGAATCCGAGTATCTGGCGGGCGACACGTATACGATC
GCGGACATCGCGACGTTCCCGTGGACGCGCTCATGGCAGAACCAGGGCATCGTGCTCGACGCGTTGCCGAACGTGAAGCG
CTGGCACGAGACGATCGCCGCGCGACCGGCCGTGCAGCGCGGCGTCGAGGTGCTCGCGTCGATGCGCAAGGCGCTGCAGG
ACGACAAGGCACGCGAGGTGCTGTTCGGCGCAACGCAATACGCGAAGCGCTGA

Upstream 100 bases:

>100_bases
GCGTCTGCGTCGCCAGCAGCCCGTGTCGATCCATGCTCTAATGACCGCCTGTCGCGGCGCCGCCGGCGCCGTTTTCGCGC
ATGCCACACAGGAGTTGACG

Downstream 100 bases:

>100_bases
CCCGCACGAGGCGGGCGGAGCGGCATCCGTGCATGCCGCTCCGTGCTTCAGAAGTAATGCAGCGTGATGAACTCCGCCGC
GCACACCGGCAGCGGCGCGT

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MIDVYSWATPNGHKVHIMLEETGLAYRAHPVDIGAGDQFEPEFLKISPNNKIPAIVDPDGPGGQSISLFESGAILIYLAE
KTGKFLPTDPAARYATLEWLMFQMGGVGPMLGQAHHFRLYAPEKIEYAVNRYTNEAKRLYNVMEKRLGESEYLAGDTYTI
ADIATFPWTRSWQNQGIVLDALPNVKRWHETIAARPAVQRGVEVLASMRKALQDDKAREVLFGATQYAKR

Sequences:

>Translated_230_residues
MIDVYSWATPNGHKVHIMLEETGLAYRAHPVDIGAGDQFEPEFLKISPNNKIPAIVDPDGPGGQSISLFESGAILIYLAE
KTGKFLPTDPAARYATLEWLMFQMGGVGPMLGQAHHFRLYAPEKIEYAVNRYTNEAKRLYNVMEKRLGESEYLAGDTYTI
ADIATFPWTRSWQNQGIVLDALPNVKRWHETIAARPAVQRGVEVLASMRKALQDDKAREVLFGATQYAKR
>Mature_230_residues
MIDVYSWATPNGHKVHIMLEETGLAYRAHPVDIGAGDQFEPEFLKISPNNKIPAIVDPDGPGGQSISLFESGAILIYLAE
KTGKFLPTDPAARYATLEWLMFQMGGVGPMLGQAHHFRLYAPEKIEYAVNRYTNEAKRLYNVMEKRLGESEYLAGDTYTI
ADIATFPWTRSWQNQGIVLDALPNVKRWHETIAARPAVQRGVEVLASMRKALQDDKAREVLFGATQYAKR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=218, Percent_Identity=54.5871559633027, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI87082195, Length=214, Percent_Identity=48.1308411214953, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI87081789, Length=187, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324100, Length=234, Percent_Identity=34.6153846153846, Blast_Score=128, Evalue=8e-31,
Organism=Drosophila melanogaster, GI17933730, Length=222, Percent_Identity=26.5765765765766, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17864592, Length=223, Percent_Identity=30.0448430493274, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571670, Length=173, Percent_Identity=27.7456647398844, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17864598, Length=167, Percent_Identity=32.9341317365269, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI85725204, Length=195, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17737923, Length=195, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17864594, Length=192, Percent_Identity=28.6458333333333, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45549270, Length=192, Percent_Identity=27.6041666666667, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17864596, Length=194, Percent_Identity=25.7731958762887, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646251, Length=176, Percent_Identity=28.9772727272727, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19922160, Length=189, Percent_Identity=26.984126984127, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646249, Length=164, Percent_Identity=27.4390243902439, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 25797; Mature: 25797

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVYSWATPNGHKVHIMLEETGLAYRAHPVDIGAGDQFEPEFLKISPNNKIPAIVDPDG
CCCEEECCCCCCCEEEEEEECCCCEEEECEEECCCCCCCCCCEEEECCCCCCCEEECCCC
PGGQSISLFESGAILIYLAEKTGKFLPTDPAARYATLEWLMFQMGGVGPMLGQAHHFRLY
CCCCEEEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCEEEEE
APEKIEYAVNRYTNEAKRLYNVMEKRLGESEYLAGDTYTIADIATFPWTRSWQNQGIVLD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEEEECCCCCCCCCCCEEEE
ALPNVKRWHETIAARPAVQRGVEVLASMRKALQDDKAREVLFGATQYAKR
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDVYSWATPNGHKVHIMLEETGLAYRAHPVDIGAGDQFEPEFLKISPNNKIPAIVDPDG
CCCEEECCCCCCCEEEEEEECCCCEEEECEEECCCCCCCCCCEEEECCCCCCCEEECCCC
PGGQSISLFESGAILIYLAEKTGKFLPTDPAARYATLEWLMFQMGGVGPMLGQAHHFRLY
CCCCEEEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCEEEEE
APEKIEYAVNRYTNEAKRLYNVMEKRLGESEYLAGDTYTIADIATFPWTRSWQNQGIVLD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEEEECCCCCCCCCCCEEEE
ALPNVKRWHETIAARPAVQRGVEVLASMRKALQDDKAREVLFGATQYAKR
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]