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The map label for this gene is ntrB [H]

Identifier: 116690169

GI number: 116690169

Start: 2388526

End: 2389668

Strand: Reverse

Name: ntrB [H]

Synonym: Bcen2424_2148

Alternate gene names: 116690169

Gene position: 2389668-2388526 (Counterclockwise)

Preceding gene: 116690170

Following gene: 116690168

Centisome position: 68.59

GC content: 65.97

Gene sequence:

>1143_bases
ATGGTTCTGAAGAATCTGATCAAGGCGAAAACGGGGCAACCCGAACGACTGACGGACGACGAGCGGCTCGCGCGCTCGGG
TCTGCTGGCGGGGCTCGAAGCGCTGCCGACGGTCGTGATCGTGCTCGACCGCAAGACGCTGCGGGTCGCGTTCGCGAACC
CGTCCGCGGAGGCGATGCTCGACATCTCGCGGCGGCAGCTCGCGCAGCGGCCGTGGGGCGAAATTTTCCCGAACGCGAAC
GAGCTTGCGTCGACGATCACCGCGATCGGCCAGGAACGCTTTCACGCGACGCACCTCGATACGGTGCTCGACCGGCCCGG
CCGCGAACCGCTGCATGTGCATGCGATCGTCGGCTTCCTCGAGAGCGCGCCCGATTTCGTGCTCGTCGAGCTGTTCGAGA
ACGAACGGCAGTCGCGCACCGATCGGGAAGAGCGCATCCACGACCTGACCGCGGTCAACAAGCAACTGATCCGCAACCTC
GCGCACGAGATCAAGAACCCGCTCGGCGGCATTCGCGGCGCAGCCCAGCTGCTCGAATTCGAACTGGGCGAGCGCGAGCG
CGGCGAGTTGCGCGAATACACGCAGGTGATCATCAAGGAGTCCGATCGCCTGCAGACGCTCGTCGACCGGCTGCTGGAGC
CGCACCGGCATCCGCACATCGTCGGCGACGTGAACATTCATGAAGTGTGCGAACGCGTGCGCGCGGTGATGCTCGCGGAG
TTCCCGCGCGGGCTCACGATCGAGCGCGATTACGACGTGAGCGTGCCGGACCTGCGCGGCGACAAGGAGCAGTTGATCCA
GGCGCTGCTCAACATCGTGCGCAACGCCGCGCAGGCGCTGCGCGAGCGGATCGCGCAGGGCGACGCGAAGATCGAGCTGC
GCACGCGCATCGCGCGCAAGATCACGATCGCGAAGCGCCTGTACAAGCTGGCACTGGACTTGCACGTGGTCGACAACGGG
CCCGGCATTCCGGAAGAGATCCGCGACCGGATCTTCTACCCGCTCGTGTCCGGACGCGACGACGGCAGCGGCCTCGGCCT
CACGCTCGCACAGACGTTCGTGCAGCAGCACGACGGGATGATCGAGGTCGAAAGCCGGCCCGGACGTACCGAATTTCAGA
TTCTGCTGCCGCTCGACCATTGA

Upstream 100 bases:

>100_bases
GCGGGAACGGCGGAGTCTGAAAGGGGACGGCGTCCGGCCGTCCCTTTTTTGTTCCGACGATTTTCATTTTCTGGCCCGGT
GAGGCGCGACAAGCACGCAG

Downstream 100 bases:

>100_bases
GCGGCGACAGTGCGATTCACCCATACCTGACCGACCTATGAAGCCGATCTGGATAGTAGACGACGACCAATCGATCCGCT
GGGTGCTTGAAAAGGCACTC

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MVLKNLIKAKTGQPERLTDDERLARSGLLAGLEALPTVVIVLDRKTLRVAFANPSAEAMLDISRRQLAQRPWGEIFPNAN
ELASTITAIGQERFHATHLDTVLDRPGREPLHVHAIVGFLESAPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRIARKITIAKRLYKLALDLHVVDNG
PGIPEEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGMIEVESRPGRTEFQILLPLDH

Sequences:

>Translated_380_residues
MVLKNLIKAKTGQPERLTDDERLARSGLLAGLEALPTVVIVLDRKTLRVAFANPSAEAMLDISRRQLAQRPWGEIFPNAN
ELASTITAIGQERFHATHLDTVLDRPGREPLHVHAIVGFLESAPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRIARKITIAKRLYKLALDLHVVDNG
PGIPEEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGMIEVESRPGRTEFQILLPLDH
>Mature_380_residues
MVLKNLIKAKTGQPERLTDDERLARSGLLAGLEALPTVVIVLDRKTLRVAFANPSAEAMLDISRRQLAQRPWGEIFPNAN
ELASTITAIGQERFHATHLDTVLDRPGREPLHVHAIVGFLESAPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRIARKITIAKRLYKLALDLHVVDNG
PGIPEEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGMIEVESRPGRTEFQILLPLDH

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=351, Percent_Identity=41.5954415954416, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1788549, Length=353, Percent_Identity=28.0453257790368, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790436, Length=267, Percent_Identity=28.8389513108614, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=27.5720164609054, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082128, Length=245, Percent_Identity=26.530612244898, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43004; Mature: 43004

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLKNLIKAKTGQPERLTDDERLARSGLLAGLEALPTVVIVLDRKTLRVAFANPSAEAML
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCHHHHH
DISRRQLAQRPWGEIFPNANELASTITAIGQERFHATHLDTVLDRPGREPLHVHAIVGFL
HHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ESAPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEF
HCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRIARK
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
ITIAKRLYKLALDLHVVDNGPGIPEEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGM
HHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCE
IEVESRPGRTEFQILLPLDH
EEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MVLKNLIKAKTGQPERLTDDERLARSGLLAGLEALPTVVIVLDRKTLRVAFANPSAEAML
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCHHHHH
DISRRQLAQRPWGEIFPNANELASTITAIGQERFHATHLDTVLDRPGREPLHVHAIVGFL
HHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ESAPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEF
HCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRIARK
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
ITIAKRLYKLALDLHVVDNGPGIPEEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGM
HHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCE
IEVESRPGRTEFQILLPLDH
EEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]