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The map label for this gene is glnA [H]

Identifier: 116690170

GI number: 116690170

Start: 2389830

End: 2391245

Strand: Reverse

Name: glnA [H]

Synonym: Bcen2424_2149

Alternate gene names: 116690170

Gene position: 2391245-2389830 (Counterclockwise)

Preceding gene: 116690172

Following gene: 116690169

Centisome position: 68.64

GC content: 62.43

Gene sequence:

>1416_bases
ATGAGTAAAACCGTCGCCGACGTCATGCAGCTCGTGAAGGACGAGGACGTCAAGTTTGTCGATTTCCGCTTCACGGATAC
GCGCGGCAAGGAACAGCACGTGTCGGTGCCGGTTTCGGCGTTTGACGAAGACAAGTTCGAAAGCGGCCATGCATTCGACG
GTTCGTCGATCGCGGGCTGGAAGGGCATCGAGGCGTCGGACATGCTGCTCATGCCGGACCCGACCGCTGCCTTCGTCGAC
CCGTTCTACGAAGAGTCGACCCTCGTGCTGACCTGCGACGTGGTCGAGCCGGCCGACGGCAAGGGCTACGAGCGCGACCC
GCGTTCGCTCGCGAAGCGCGCGGAAGCCTACCTTAAGAGCACGGGTATCGGCGACACGGCGTACTTCGGTCCGGAACCGG
AATTCTTCATTTTCGACTCGGTCCAGTGGAACACGGACATGTCGGGCTGCTTCGTGAAGATCAACTCGGAAGAAGCACCG
TGGTCGGCCGGCAAGGAATTCGAAGGCGGCAACACCGGCCACCGTCCGGGCACAAAGGGCGGCTACTTCCCGGTCGCGCC
GGTCGACACGTTCCAGGACATGCGTTCGGAAATGTGCCTGCTGCTCGAACAGCTCGGCATCCCGGTCGAAGTGCACCACC
ACGAAGTGGCGGGCCAGGGCCAGAACGAAATCGGCACGAAGTTCTCGACGCTGGTTCAGCGCGCCGACTGGACGCAATGG
TCGAAGTACATCATCCATAACGTCGCGCACTCGTACGGCAAGACGGCGACGTTCATGCCGAAGCCGGTCGTCGGCGACAA
CGGTTCGGGCATGCACGTTCACCAGTCGATCTGGAAGGACGGCCAGAACCTGTTCGCGGGCAACGGCTACGCCGGCCTGT
CGGAACTGGCGCTGTTCTACATCGGCGGCATCATCAAGCACGCCCGTGCGCTGAACGCGATCACGAACCCGACGACGAAC
TCGTACAAGCGTCTGGTCCCGCACTTCGAAGCACCGGTCAAGCTCGCCTACTCGGCACGCAACCGTTCGGCATCGATCCG
CATTCCGCACGTGTCGAACCCGAAGGGCCGCCGTATCGAAACGCGCTTCCCGGACCCGATGGCGAACCCGTACCTGTTGT
TCTCGGCGCTGATGATGGCCGGCCTCGACGGGATCCAGAACAAGATCCATCCGGGCGAGGCAGCGGACAAGAACCTGTAC
GACCTGCCGCCGGAAGAGGATGCAAAGATCCCGACCGTCTGCGCAGGCCTCGACCAGGCACTGGAAGCGCTCGACAAGGA
TCGCGAGTTCCTGACCCGTGGCGGCGTGTTCACGGACAGCATGCTCGACGCGTACCTCGCGCTGAAGGAGCAGGAGCTGG
CGAAGTTCCGCATGACGACGCACCCGATCGAGTTCGAGATGTACTACTCGCTCTAA

Upstream 100 bases:

>100_bases
GTCGAATCGGGGCGCGCTGCGCCAAAGCAAGACCCCGATCAGCTCGATAAACGAGGCGGTTTCCCAGTCCGCCGGAATTG
TTCAATCAGGAGAAGAGGTT

Downstream 100 bases:

>100_bases
TCGGCGATGGCGCTTCGATTCAGCGCCGTCATGCCGGTTCGTTCCCGTCGCGCGGCTGGCGGCGGGAACGGCGGAGTCTG
AAAGGGGACGGCGTCCGGCC

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSKTVADVMQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGWKGIEASDMLLMPDPTAAFVD
PFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGIGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAP
WSAGKEFEGGNTGHRPGTKGGYFPVAPVDTFQDMRSEMCLLLEQLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQW
SKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSELALFYIGGIIKHARALNAITNPTTN
SYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLLFSALMMAGLDGIQNKIHPGEAADKNLY
DLPPEEDAKIPTVCAGLDQALEALDKDREFLTRGGVFTDSMLDAYLALKEQELAKFRMTTHPIEFEMYYSL

Sequences:

>Translated_471_residues
MSKTVADVMQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGWKGIEASDMLLMPDPTAAFVD
PFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGIGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAP
WSAGKEFEGGNTGHRPGTKGGYFPVAPVDTFQDMRSEMCLLLEQLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQW
SKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSELALFYIGGIIKHARALNAITNPTTN
SYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLLFSALMMAGLDGIQNKIHPGEAADKNLY
DLPPEEDAKIPTVCAGLDQALEALDKDREFLTRGGVFTDSMLDAYLALKEQELAKFRMTTHPIEFEMYYSL
>Mature_470_residues
SKTVADVMQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGWKGIEASDMLLMPDPTAAFVDP
FYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGIGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAPW
SAGKEFEGGNTGHRPGTKGGYFPVAPVDTFQDMRSEMCLLLEQLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQWS
KYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSELALFYIGGIIKHARALNAITNPTTNS
YKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLLFSALMMAGLDGIQNKIHPGEAADKNLYD
LPPEEDAKIPTVCAGLDQALEALDKDREFLTRGGVFTDSMLDAYLALKEQELAKFRMTTHPIEFEMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=64.6551724137931, Blast_Score=666, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=400, Percent_Identity=25.75, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52260; Mature: 52129

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTVADVMQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGW
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMLLMPDPTAAFVDPFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKS
CCCCCCCEEEECCCCHHHHCHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TGIGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAPWSAGKEFEGGNTGHRPGTKG
CCCCCCCCCCCCCCEEEEECEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GYFPVAPVDTFQDMRSEMCLLLEQLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQW
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCCHHHHHHHHHHHHHHCHHHH
SKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSELALFY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPTTNSYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIE
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEEE
TRFPDPMANPYLLFSALMMAGLDGIQNKIHPGEAADKNLYDLPPEEDAKIPTVCAGLDQA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LEALDKDREFLTRGGVFTDSMLDAYLALKEQELAKFRMTTHPIEFEMYYSL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
SKTVADVMQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGW
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMLLMPDPTAAFVDPFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKS
CCCCCCCEEEECCCCHHHHCHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TGIGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAPWSAGKEFEGGNTGHRPGTKG
CCCCCCCCCCCCCCEEEEECEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GYFPVAPVDTFQDMRSEMCLLLEQLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQW
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCCHHHHHHHHHHHHHHCHHHH
SKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSELALFY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPTTNSYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIE
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEEE
TRFPDPMANPYLLFSALMMAGLDGIQNKIHPGEAADKNLYDLPPEEDAKIPTVCAGLDQA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LEALDKDREFLTRGGVFTDSMLDAYLALKEQELAKFRMTTHPIEFEMYYSL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]