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The map label for this gene is glnG [H]

Identifier: 116690168

GI number: 116690168

Start: 2386974

End: 2388488

Strand: Reverse

Name: glnG [H]

Synonym: Bcen2424_2147

Alternate gene names: 116690168

Gene position: 2388488-2386974 (Counterclockwise)

Preceding gene: 116690169

Following gene: 116690167

Centisome position: 68.56

GC content: 69.83

Gene sequence:

>1515_bases
ATGAAGCCGATCTGGATAGTAGACGACGACCAATCGATCCGCTGGGTGCTTGAAAAGGCACTCGCCCGGGACAGCTTCGC
GACGAAGAGCTTCGCGAACGTGCGCGATGCGCTGGCCGCGCTCGACCACGAGACGCCGCAGGTGCTCGTGTCCGACATCC
GGATGCCGGGCGGCTCGGGCCTCGAGTTGCTGCAGGCGATGCACGAGCGGCTGCCGGGTCTGCCCGTCATCATCATGACG
GCGTTCTCCGATCTCGACAGCGCCGTCGCGGCGTTCCAGGGCGGCGCGTTCGAATACCTCGCGAAGCCGTTCGACGTCGA
CAAGGCGGTCGAGCTGATTCGCCGTGCAGTGGAAGAAAGCCTGCGCGGCGGCGCGCCGCAGGACGAGCGCGTGGCCGAGG
CGCCCGAGATGCTCGGCCAGGCGCCCGCGATGCAGGACATGTTCCGCGCGATCGGCCGCTTGTCGCATTCGGCCGCGACC
GTGCTGATCACCGGCGAGTCGGGCACCGGCAAGGAACTCGTCGCGCGGGCGCTGCACCGTCACAGCCCGCGTGCGAACGG
ACCGTTCATCGCGCTGAACACGGCGGCGATTCCGAAGGATCTGCTTGAATCCGAACTGTTCGGCCACGAGCGCGGCGCGT
TCACCGGCGCGCAGACGACGCGCCAGGGCCGCTTCGAGCAGGCCGAGAACGGCACGCTGTTCCTCGACGAAATCGGCGAC
ATGCCGTTCGATCTGCAGACGCGCCTGTTGCGCGTGCTGTCGGACGGGCAGTTCTACCGCGTCGGCGGGCACAACCCGCT
GCGCGCGAACGTGCGCGTGATCGCCGCGACGCACCAGAATCTCGAGTCGCGCGTGCGGCAGGGGCTGTTCCGCGAGGACC
TTTACCACCGGCTCAACGTGATCCGGCTGCGTCTGCCGCCGCTGCGCGAACGCAGCGAGGACATCGCGCTGCTCACGCGC
CACTTCCTGCAGAAGAGCGCGCGCGATCTCGGCGTCGAGCCGAAGCGCGTGTCCGACGACGCGCTCGCGTACCTGACGTC
GCTGCCGTTTCCCGGCAACGTGCGGCAGCTCGAGAATCTCGCGAACTGGCTGACCGTGATGGCGCCCGCGCAGACGGTCG
AGATCAAGGATCTGCCGCCCGACCTCGTGCCGGCCGGTGCGCCGGTCGTCGCGACGGGCGACGGCGCGGACGCGCACGGC
AGCGGCGGTCACGCGGCCGCGGTGCCTTCCGCGATCGGTGCAGCGCCGACGGCCGCGCCCGTCGCGAGCGCGGCGAACGG
CAGCGCGCCGGCCGGCTACCCGTTGTGGGAGCACGGGCTGCGCACCGAAGTCGCGCGGCTGTTGCGCGAGAACTCGGCCG
ACGTGATGGACGAACTCGCACGCCGCTTCGAAGCCGCCGTGATCCGTGAGGCGCTCGACTTCACGCGCGGTCGCAAGGTC
GAGGCCGCGGAGCGGCTCGGGATCGGCCGCAACACGATCACGCGCAAGATCCAGGAACTCCATCTGGAGCCCTGA

Upstream 100 bases:

>100_bases
ATCGAGGTCGAAAGCCGGCCCGGACGTACCGAATTTCAGATTCTGCTGCCGCTCGACCATTGAGCGGCGACAGTGCGATT
CACCCATACCTGACCGACCT

Downstream 100 bases:

>100_bases
GCACGATTCGCGGCATGCGCGGGCCCGCGCATGCCGCGATCGGACATAATCGCGGTTTGCATGCAGCTTGCCATTCCGCG
ATCCTCAATGTCCGAATCCT

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSGLELLQAMHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGAPQDERVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPPLRERSEDIALLTR
HFLQKSARDLGVEPKRVSDDALAYLTSLPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVPAGAPVVATGDGADAHG
SGGHAAAVPSAIGAAPTAAPVASAANGSAPAGYPLWEHGLRTEVARLLRENSADVMDELARRFEAAVIREALDFTRGRKV
EAAERLGIGRNTITRKIQELHLEP

Sequences:

>Translated_504_residues
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSGLELLQAMHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGAPQDERVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPPLRERSEDIALLTR
HFLQKSARDLGVEPKRVSDDALAYLTSLPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVPAGAPVVATGDGADAHG
SGGHAAAVPSAIGAAPTAAPVASAANGSAPAGYPLWEHGLRTEVARLLRENSADVMDELARRFEAAVIREALDFTRGRKV
EAAERLGIGRNTITRKIQELHLEP
>Mature_504_residues
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSGLELLQAMHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGAPQDERVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPPLRERSEDIALLTR
HFLQKSARDLGVEPKRVSDDALAYLTSLPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVPAGAPVVATGDGADAHG
SGGHAAAVPSAIGAAPTAAPVASAANGSAPAGYPLWEHGLRTEVARLLRENSADVMDELARRFEAAVIREALDFTRGRKV
EAAERLGIGRNTITRKIQELHLEP

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=501, Percent_Identity=55.688622754491, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1788550, Length=506, Percent_Identity=39.3280632411067, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1790437, Length=387, Percent_Identity=42.1188630490956, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI1788905, Length=371, Percent_Identity=41.7789757412399, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI87082117, Length=225, Percent_Identity=52.8888888888889, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1789233, Length=380, Percent_Identity=38.1578947368421, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789087, Length=314, Percent_Identity=42.3566878980892, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=48.936170212766, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1786524, Length=262, Percent_Identity=46.9465648854962, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=253, Percent_Identity=44.2687747035573, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=45.575221238938, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=35.8407079646018, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081858, Length=228, Percent_Identity=34.2105263157895, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1789809, Length=174, Percent_Identity=27.0114942528736, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54848; Mature: 54848

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
LELLQAMHERLPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LRGGAPQDERVAEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCHHCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHE
IRLRLPPLRERSEDIALLTRHFLQKSARDLGVEPKRVSDDALAYLTSLPFPGNVRQLENL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
ANWLTVMAPAQTVEIKDLPPDLVPAGAPVVATGDGADAHGSGGHAAAVPSAIGAAPTAAP
HHHHHHHCCCCEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHCCHHHCCCCCHHH
VASAANGSAPAGYPLWEHGLRTEVARLLRENSADVMDELARRFEAAVIREALDFTRGRKV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAAERLGIGRNTITRKIQELHLEP
HHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
LELLQAMHERLPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LRGGAPQDERVAEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCHHCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHE
IRLRLPPLRERSEDIALLTRHFLQKSARDLGVEPKRVSDDALAYLTSLPFPGNVRQLENL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
ANWLTVMAPAQTVEIKDLPPDLVPAGAPVVATGDGADAHGSGGHAAAVPSAIGAAPTAAP
HHHHHHHCCCCEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHCCHHHCCCCCHHH
VASAANGSAPAGYPLWEHGLRTEVARLLRENSADVMDELARRFEAAVIREALDFTRGRKV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAAERLGIGRNTITRKIQELHLEP
HHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]