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The map label for this gene is nuoM [H]

Identifier: 116690093

GI number: 116690093

Start: 2299464

End: 2300993

Strand: Reverse

Name: nuoM [H]

Synonym: Bcen2424_2072

Alternate gene names: 116690093

Gene position: 2300993-2299464 (Counterclockwise)

Preceding gene: 116690094

Following gene: 116690092

Centisome position: 66.05

GC content: 65.75

Gene sequence:

>1530_bases
ATGCATGATTTCCCCTTCCTGAGCCTGGCCATCTGGGTTCCGATACTGTTCGGCGCGGGGCTGCTGCGCGCCGGTTCCGA
CCAGCATTCACGCCGGATACGACTGATCGCGCTGGTCGGCGCGATCGCCGGGCTGGCGGCCGTCGTGCCGCTGATCGCCG
GTTTCGACGCGCATTCGGCGGCGATGCAGTTCGTCGAAAGACGCGACTGGCTGGCGGCATTCAATGCCGGCTGGCGGGTC
GGCATCGACGGCGCGTCGCTGTGGCTCGTCGTGCTGACCGCATTCACGACGCTCGTGGTGGTGGTGGCGTCGTGGGAGTC
GGTCACGGTGCGCGTCGCGCAGTACTACGCGTCGTTCCTGATCCTGTCGGGGCTGATGGTCGGCGTATTCGTCGCGCAGG
ACGGGCTGCTGTTCTTCATCTTCTTCGAGGCGACGCTGATCCCGCTGTACCTGCTGATCGGTACGTGGGGGCGCGAGCGA
CGCGTGTATGCGGCCGTGAAGTTCTTCTTCATCTCGTTCGCCGGCTCGCTGTTGTTGCTGATGGCGATGCTGTACCTGTA
CGCGCAGTCGCATACGTTCGACATGGCCGTGTGGCGCACGCTGCAGCTCGGCTTTGCGCCGCAGTTGCTGGTGTTCCTCG
GCTTCTTCGCGGCGTTCGCGGTCAAGGTGCCGATGTGGCCGGTCCACACGTGGCTGAGCGACGTGTATACGGACGGTCCG
ACGGGTGCCGCGCTGATGCTCGCGATGCTCAAGCTCGGCGGTTACGGGTTCCTGCGCTTCGCGCTGCCGATCACGCCGGA
CGCGAGCCACTTCTTCGCGCCGGCCGTGATCGCGCTGTCGCTGTTCGCGATCGTCTACGCGAGCCTGATCGCGCTCGCGC
AGACCGATCTCGGCAAGCTGCTCGCGTATTCGACGGTCGCGCACATGGGGATCGTCACGCTGGGGCTGTTCCTGTTCAAC
CGGATCGGCGTCGAAGGCGCGATCGTGCAACTCGTGTCGTACGGCTTCGTGGCGGGCGCGATGCTGTTGTGCGTGAACGT
GCTCGTGGATCGCACCAGGCAGCGCGAGATTGCAGCGTACGGCGGCGTGGCGGGCGTGATGCCGCGTTTCGCGACCTTCA
CGCTGCTGTTCGCGATGGCCAACGTCGGTCTGCCGGGCACGTCGGGGTTCGTCGGCGAGTTCATGGTGATCATGGGCGCG
ATCCGCTTCAACTTCTGGATCGGTGCGATCGCGGCGCTCACGCTGATCCTCAGCGCGTCGTACACGCTGTGGATGCTCAA
GCGCGTCGTGTTCGGCAAGGTCGCGAGCCAGCGGATCGCGAAGCTCGCCGACCTGAATCGTCGCGAGATCGTGATGTTCG
CGTCGCTCGCGCTGATCGTGCTGGCGGTCGGCATCGATCCGAAGCCGTTCACCGATGCGATCGATCCGACCGTCGGCAAG
CTGATCCAGGAAGCGAGCCATTCGAAGGTGCCGGCAGGGGATGGCCCCGCGCCGGCGCTGCCGTCGTACATGGCGTCGAC
GCACGACTGA

Upstream 100 bases:

>100_bases
AACATTGGCGATTCACGAAATCTACCTGTCGATTTGATTTCGTTCCTTTCGTCGCGCCTGTCCCCGATGCAGGGCGCGAC
CTCCTCAAGGCGTTGTTGAC

Downstream 100 bases:

>100_bases
TCCCGGCACACCGCGCCGCGCAGGCGAGCCGCATACGCGCGGCAGGTATCGTTGCGCGCGCATCGATCGTGGCCCGGCAC
GGGGCGTGCCGGGTCCGCTT

Product: proton-translocating NADH-quinone oxidoreductase, chain M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MHDFPFLSLAIWVPILFGAGLLRAGSDQHSRRIRLIALVGAIAGLAAVVPLIAGFDAHSAAMQFVERRDWLAAFNAGWRV
GIDGASLWLVVLTAFTTLVVVVASWESVTVRVAQYYASFLILSGLMVGVFVAQDGLLFFIFFEATLIPLYLLIGTWGRER
RVYAAVKFFFISFAGSLLLLMAMLYLYAQSHTFDMAVWRTLQLGFAPQLLVFLGFFAAFAVKVPMWPVHTWLSDVYTDGP
TGAALMLAMLKLGGYGFLRFALPITPDASHFFAPAVIALSLFAIVYASLIALAQTDLGKLLAYSTVAHMGIVTLGLFLFN
RIGVEGAIVQLVSYGFVAGAMLLCVNVLVDRTRQREIAAYGGVAGVMPRFATFTLLFAMANVGLPGTSGFVGEFMVIMGA
IRFNFWIGAIAALTLILSASYTLWMLKRVVFGKVASQRIAKLADLNRREIVMFASLALIVLAVGIDPKPFTDAIDPTVGK
LIQEASHSKVPAGDGPAPALPSYMASTHD

Sequences:

>Translated_509_residues
MHDFPFLSLAIWVPILFGAGLLRAGSDQHSRRIRLIALVGAIAGLAAVVPLIAGFDAHSAAMQFVERRDWLAAFNAGWRV
GIDGASLWLVVLTAFTTLVVVVASWESVTVRVAQYYASFLILSGLMVGVFVAQDGLLFFIFFEATLIPLYLLIGTWGRER
RVYAAVKFFFISFAGSLLLLMAMLYLYAQSHTFDMAVWRTLQLGFAPQLLVFLGFFAAFAVKVPMWPVHTWLSDVYTDGP
TGAALMLAMLKLGGYGFLRFALPITPDASHFFAPAVIALSLFAIVYASLIALAQTDLGKLLAYSTVAHMGIVTLGLFLFN
RIGVEGAIVQLVSYGFVAGAMLLCVNVLVDRTRQREIAAYGGVAGVMPRFATFTLLFAMANVGLPGTSGFVGEFMVIMGA
IRFNFWIGAIAALTLILSASYTLWMLKRVVFGKVASQRIAKLADLNRREIVMFASLALIVLAVGIDPKPFTDAIDPTVGK
LIQEASHSKVPAGDGPAPALPSYMASTHD
>Mature_509_residues
MHDFPFLSLAIWVPILFGAGLLRAGSDQHSRRIRLIALVGAIAGLAAVVPLIAGFDAHSAAMQFVERRDWLAAFNAGWRV
GIDGASLWLVVLTAFTTLVVVVASWESVTVRVAQYYASFLILSGLMVGVFVAQDGLLFFIFFEATLIPLYLLIGTWGRER
RVYAAVKFFFISFAGSLLLLMAMLYLYAQSHTFDMAVWRTLQLGFAPQLLVFLGFFAAFAVKVPMWPVHTWLSDVYTDGP
TGAALMLAMLKLGGYGFLRFALPITPDASHFFAPAVIALSLFAIVYASLIALAQTDLGKLLAYSTVAHMGIVTLGLFLFN
RIGVEGAIVQLVSYGFVAGAMLLCVNVLVDRTRQREIAAYGGVAGVMPRFATFTLLFAMANVGLPGTSGFVGEFMVIMGA
IRFNFWIGAIAALTLILSASYTLWMLKRVVFGKVASQRIAKLADLNRREIVMFASLALIVLAVGIDPKPFTDAIDPTVGK
LIQEASHSKVPAGDGPAPALPSYMASTHD

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=368, Percent_Identity=28.804347826087, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI251831117, Length=351, Percent_Identity=23.9316239316239, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788613, Length=428, Percent_Identity=34.3457943925234, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1788827, Length=284, Percent_Identity=28.169014084507, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1788829, Length=384, Percent_Identity=27.8645833333333, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI145693160, Length=408, Percent_Identity=25.9803921568627, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788831, Length=318, Percent_Identity=25.1572327044025, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788614, Length=432, Percent_Identity=26.8518518518519, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55091; Mature: 55091

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDFPFLSLAIWVPILFGAGLLRAGSDQHSRRIRLIALVGAIAGLAAVVPLIAGFDAHSA
CCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AMQFVERRDWLAAFNAGWRVGIDGASLWLVVLTAFTTLVVVVASWESVTVRVAQYYASFL
HHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ILSGLMVGVFVAQDGLLFFIFFEATLIPLYLLIGTWGRERRVYAAVKFFFISFAGSLLLL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
MAMLYLYAQSHTFDMAVWRTLQLGFAPQLLVFLGFFAAFAVKVPMWPVHTWLSDVYTDGP
HHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
TGAALMLAMLKLGGYGFLRFALPITPDASHFFAPAVIALSLFAIVYASLIALAQTDLGKL
CHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYSTVAHMGIVTLGLFLFNRIGVEGAIVQLVSYGFVAGAMLLCVNVLVDRTRQREIAAY
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGVAGVMPRFATFTLLFAMANVGLPGTSGFVGEFMVIMGAIRFNFWIGAIAALTLILSAS
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTLWMLKRVVFGKVASQRIAKLADLNRREIVMFASLALIVLAVGIDPKPFTDAIDPTVGK
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
LIQEASHSKVPAGDGPAPALPSYMASTHD
HHHHHCCCCCCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure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HHHHHCCCCCCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]