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The map label for this gene is thiD [H]

Identifier: 116690062

GI number: 116690062

Start: 2265806

End: 2266615

Strand: Direct

Name: thiD [H]

Synonym: Bcen2424_2041

Alternate gene names: 116690062

Gene position: 2265806-2266615 (Clockwise)

Preceding gene: 116690061

Following gene: 116690067

Centisome position: 65.04

GC content: 70.49

Gene sequence:

>810_bases
ATGACGCACCCGATACCCAACCTCCTGACGATCGCCGGCTCCGATTCGGGCGGCGGCGCAGGCATTCAGGCCGACCTGAA
GACGTTTTCGGCGCTCGGCGCATATGGTGCGAGCGTGATCACCGCGCTGACCGCGCAGAACACGCGCGGCGTGACGGGCG
TGCATGCGCCGGACGCCGCCTTCGTGACCGCGCAGCTCGACGCGGTGTTCGACGATATCCGCATCGACGCGGTCAAGATC
GGGATGCTCGCCAACGCGGCGATCGTGCAGGCGGTGGCCGACGCGCTGCGTCGTTACGCGCCGCGCTTCGTGGTGCTCGA
CACGGTGATGATCTCGAAGAGTTCGCACGCGCTGCTCGCGCCCGATGCCGTCGACGCGCTGCGCGACACGCTGCTGCCGC
TCGCGACGGTCGTCACGCCGAACCTGCCGGAAGCGGCCGCGCTGCTGAACGACGCGCCCGCCACCACCGAAGACGACATG
GTGCGGCAAGGCGAGGCCCTGCTGCAGACAGGCGCGCGCGCGGTGCTGATGAAGGGCGGCCACCTGCCCGACGCGTCCGC
GAGCCCCGACTGGCTCGTCGAGGAAACCCGCACCGTGCGGCTCGACGGCGCGCGCGTGCCGGTCAGCAACACGCACGGCA
CCGGCTGCACGCTGTCGTCGGCAATCGCCGCGCTGCTGCCGCAGCAGTCCGACCTCGAAAGCGCGGTACGCGAGGCAAAA
ACCTACCTGACCGGCGCGATCGCCGCGAGCGGCCACCTCGACGTCGGTCACGGCGTCGGCCCGGTCCATCACTTCCATCG
CTGGTGGTGA

Upstream 100 bases:

>100_bases
CAGTTCGACTCGGTCGCGCTGATCGGCGAATCGCGGCACTGACGCTACACTTTCCGTTTTCGTCCGCCGGCGCCGGTGCT
CACACCGGCGCGTTGCCCTC

Downstream 100 bases:

>100_bases
GCGGCCGCTGACGCGGCGTCAGTGATCCTGCGCCGCGAACGCCTGCGCGCGCGCCGGCCAGTCGTCGGGCACGACGAAAC
CGCGCGCCGTCTCGCGCCAC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTHPIPNLLTIAGSDSGGGAGIQADLKTFSALGAYGASVITALTAQNTRGVTGVHAPDAAFVTAQLDAVFDDIRIDAVKI
GMLANAAIVQAVADALRRYAPRFVVLDTVMISKSSHALLAPDAVDALRDTLLPLATVVTPNLPEAAALLNDAPATTEDDM
VRQGEALLQTGARAVLMKGGHLPDASASPDWLVEETRTVRLDGARVPVSNTHGTGCTLSSAIAALLPQQSDLESAVREAK
TYLTGAIAASGHLDVGHGVGPVHHFHRWW

Sequences:

>Translated_269_residues
MTHPIPNLLTIAGSDSGGGAGIQADLKTFSALGAYGASVITALTAQNTRGVTGVHAPDAAFVTAQLDAVFDDIRIDAVKI
GMLANAAIVQAVADALRRYAPRFVVLDTVMISKSSHALLAPDAVDALRDTLLPLATVVTPNLPEAAALLNDAPATTEDDM
VRQGEALLQTGARAVLMKGGHLPDASASPDWLVEETRTVRLDGARVPVSNTHGTGCTLSSAIAALLPQQSDLESAVREAK
TYLTGAIAASGHLDVGHGVGPVHHFHRWW
>Mature_268_residues
THPIPNLLTIAGSDSGGGAGIQADLKTFSALGAYGASVITALTAQNTRGVTGVHAPDAAFVTAQLDAVFDDIRIDAVKIG
MLANAAIVQAVADALRRYAPRFVVLDTVMISKSSHALLAPDAVDALRDTLLPLATVVTPNLPEAAALLNDAPATTEDDMV
RQGEALLQTGARAVLMKGGHLPDASASPDWLVEETRTVRLDGARVPVSNTHGTGCTLSSAIAALLPQQSDLESAVREAKT
YLTGAIAASGHLDVGHGVGPVHHFHRWW

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=263, Percent_Identity=60.0760456273764, Blast_Score=310, Evalue=7e-86,
Organism=Saccharomyces cerevisiae, GI6325378, Length=276, Percent_Identity=31.8840579710145, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324997, Length=275, Percent_Identity=32.3636363636364, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324517, Length=275, Percent_Identity=31.2727272727273, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27882; Mature: 27751

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPIPNLLTIAGSDSGGGAGIQADLKTFSALGAYGASVITALTAQNTRGVTGVHAPDAA
CCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
FVTAQLDAVFDDIRIDAVKIGMLANAAIVQAVADALRRYAPRFVVLDTVMISKSSHALLA
EEHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHEECCCCCEEEC
PDAVDALRDTLLPLATVVTPNLPEAAALLNDAPATTEDDMVRQGEALLQTGARAVLMKGG
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
HLPDASASPDWLVEETRTVRLDGARVPVSNTHGTGCTLSSAIAALLPQQSDLESAVREAK
CCCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
TYLTGAIAASGHLDVGHGVGPVHHFHRWW
HHHHHHHHCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
THPIPNLLTIAGSDSGGGAGIQADLKTFSALGAYGASVITALTAQNTRGVTGVHAPDAA
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
FVTAQLDAVFDDIRIDAVKIGMLANAAIVQAVADALRRYAPRFVVLDTVMISKSSHALLA
EEHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHEECCCCCEEEC
PDAVDALRDTLLPLATVVTPNLPEAAALLNDAPATTEDDMVRQGEALLQTGARAVLMKGG
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
HLPDASASPDWLVEETRTVRLDGARVPVSNTHGTGCTLSSAIAALLPQQSDLESAVREAK
CCCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
TYLTGAIAASGHLDVGHGVGPVHHFHRWW
HHHHHHHHCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]