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The map label for this gene is rluF [H]

Identifier: 116690043

GI number: 116690043

Start: 2240199

End: 2242208

Strand: Direct

Name: rluF [H]

Synonym: Bcen2424_2022

Alternate gene names: 116690043

Gene position: 2240199-2242208 (Clockwise)

Preceding gene: 116690042

Following gene: 116690049

Centisome position: 64.3

GC content: 70.75

Gene sequence:

>2010_bases
ATGCGCACCAAATTAACCGTCAAGAATCCGAAGCCGGCGTCGCCGACTCGCGCCCCTGTCCGCTCCGGCAGCCTCGTCGC
CCGCAAGGCGGTGCGCCCCGCCGCGCCGCCCGCGGGGGATAAGCCGGCCCGCCCGAAGAAGGCGCCCGCTGCGGCCGCCG
CTACCGGCGAGCGCTCGTTCAAGCCGCGCGGTGCCGCCGGCGCCGAGCGTCCGGGCGCGGATCGCGCACCGGCCAAGCGC
GCCCCGCGTGACGGCGGTGCCGAGCGTGGCGCGCGCGCGCCGTATCGCGAGAACGCGGCCGGTGAAGGTGCGAAGCGCAG
TTTTGGCGATCGGCGTACGTCGTCGGATCGCCCGCCGCGCCGCGATGACGATGCACGTCCGCGTCGCGCGAGTGGCGAAG
GCAGCACACGCGCGCCCTATCGCGACAACGCAGCCGGCGAAGGCGCGAAGCGCAGCTTCGGCGACCGCCGCACGTCGTCC
GATCGTCCGCCGCGCCGTGATGACGATGCACGTCCGCGCCGCGCAGGTGCCGACGAAGGCAAGCGCCCGTCCTATCGCGA
CAACGCAGCCGGCGAAGGCGCGAAGCGCAGTTTCGGCGACCGTCGCACGGCGTCCGATCGTCCGCCGCGCCGTGACGACG
ATGCGCGTCCGCGTCGCACAGGCGCCGATGAAGGCAAGCGCCCGTCCTATCGCGACAACGCGGCCGGCGAAGGTGCGAAG
CGCAGTTTCGGCGATCGGCGTACGTCTTCCGATCGACCGGCTCGCCGCGATGACGATACACGTCCGCGCCGCACGGGCAC
TGATGAAGGCAAGCGCCCGTCCTATCGCGACAACGCATCCGGCGACGGCGCGAAGCGCAGTTTTGGCGATCGCCGCACGT
CGTCCGATCGTCCGCCGCGCCGTGATGACGATGCACGTCCGCGCCGCGCAGGCGCCGACGAAGGCAAGCGCCCGTCCTAT
CGCGACAACGCATCCGGCGAAGGCGCAAAGCGCAGCTTCGGCGATCGCCGCACGTCGTCCGATCGTCCGCCGCGCCGCAG
CGACGACGATGCACGTCCGCGCCGTGCAGCAGGTGGCGACGACAGCAAGCGCCCGTCCTACCGCGACAAGTCCGCCGGCG
ACGGCGCGAAGCGCAGCTTCGGCGACCGTCCGGCCCGCCCCACGCGCGACGGCGAGCGCCGTTCGTTCGGCGCGGTGAAG
ACCGCGCAACCCGTCAAGCGCGCGGCGGCCGACGTCGACTACGGCGACGAAACGGGACTGATGCGCCTGTCGAAGCGGAT
GTCCGAACTCGGCATGTGCTCGCGCCGCGAAGCCGACGAATGGATCGAAAAGGGCTGGGTGCTCGTCGACGGCGCACGCA
TCGACACGCTCGGCACCAAGGTGCGCGCCGACCAGAAGATCGAGATCGACGAGCGTGCGAGCGCCGCGCAGGCCGCGCAA
GTGACGATCCTGCTGCACAAGCCGGTCGGCTACGTATCGGGCCAGGCGGAAGACGGCTACGAGCCGGCGGCCGTGCTGAT
CACGCGCGCGAACCGCTGGAGCGGCGACCATTCGCCGGTGCGCTTCTCCCCGCAACACCTGCATGCACTCGCACCGGCCG
GCCGTCTCGACATCGACTCCACCGGCCTGCTCGTGCTGACGCAAAACGGCGTGATCGCGAAGCAGTTGATCGGCGAACAG
TCGGACATCGACAAGGAATACCTGGTGCGCGTGCGCTTCGGCGAGCGACTGATCGACATCGACCAGCACTTCCCGGCGGA
ATCGCTCGCGAAGCTGCGCCACGGCCTCGAGCTCGACGGCGTCGCGCTGAAGCCCGCGCTGGTGAGCTGGCAGAACGGCG
AGCAGTTGCGCTTCGTGCTGCGCGAAGGCAAGAAGCGCCAGATCCGCCGCATGTGCGAACTGGTCGGCCTCGACGTGATC
GGCCTGAAACGCGTGCGCATGGGTCGCGTGATGCTCGGCGCGCTGCCGCAAGGCCAATGGCGCTATCTGTCCGCCGACGA
GACGTTCTGA

Upstream 100 bases:

>100_bases
TTCCTGCTCGCGGGCGCCGGCCTGTCCGACAACCGCCTGCAGATCCAGCTCGAAACCGAATTGCTGCGTGCGATCGCAGT
CTGAATGAATCCCAGCGTTT

Downstream 100 bases:

>100_bases
CCTCAGGCGCACCCAGTGCGCGATCCAATGAAAAAGCCCGCTCAAGCGGGCTTTTTCTTTTCCGCAGAAGCGCGGACGAT
CAGTCGTCGTCGGCCGGATC

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 669; Mature: 669

Protein sequence:

>669_residues
MRTKLTVKNPKPASPTRAPVRSGSLVARKAVRPAAPPAGDKPARPKKAPAAAAATGERSFKPRGAAGAERPGADRAPAKR
APRDGGAERGARAPYRENAAGEGAKRSFGDRRTSSDRPPRRDDDARPRRASGEGSTRAPYRDNAAGEGAKRSFGDRRTSS
DRPPRRDDDARPRRAGADEGKRPSYRDNAAGEGAKRSFGDRRTASDRPPRRDDDARPRRTGADEGKRPSYRDNAAGEGAK
RSFGDRRTSSDRPARRDDDTRPRRTGTDEGKRPSYRDNASGDGAKRSFGDRRTSSDRPPRRDDDARPRRAGADEGKRPSY
RDNASGEGAKRSFGDRRTSSDRPPRRSDDDARPRRAAGGDDSKRPSYRDKSAGDGAKRSFGDRPARPTRDGERRSFGAVK
TAQPVKRAAADVDYGDETGLMRLSKRMSELGMCSRREADEWIEKGWVLVDGARIDTLGTKVRADQKIEIDERASAAQAAQ
VTILLHKPVGYVSGQAEDGYEPAAVLITRANRWSGDHSPVRFSPQHLHALAPAGRLDIDSTGLLVLTQNGVIAKQLIGEQ
SDIDKEYLVRVRFGERLIDIDQHFPAESLAKLRHGLELDGVALKPALVSWQNGEQLRFVLREGKKRQIRRMCELVGLDVI
GLKRVRMGRVMLGALPQGQWRYLSADETF

Sequences:

>Translated_669_residues
MRTKLTVKNPKPASPTRAPVRSGSLVARKAVRPAAPPAGDKPARPKKAPAAAAATGERSFKPRGAAGAERPGADRAPAKR
APRDGGAERGARAPYRENAAGEGAKRSFGDRRTSSDRPPRRDDDARPRRASGEGSTRAPYRDNAAGEGAKRSFGDRRTSS
DRPPRRDDDARPRRAGADEGKRPSYRDNAAGEGAKRSFGDRRTASDRPPRRDDDARPRRTGADEGKRPSYRDNAAGEGAK
RSFGDRRTSSDRPARRDDDTRPRRTGTDEGKRPSYRDNASGDGAKRSFGDRRTSSDRPPRRDDDARPRRAGADEGKRPSY
RDNASGEGAKRSFGDRRTSSDRPPRRSDDDARPRRAAGGDDSKRPSYRDKSAGDGAKRSFGDRPARPTRDGERRSFGAVK
TAQPVKRAAADVDYGDETGLMRLSKRMSELGMCSRREADEWIEKGWVLVDGARIDTLGTKVRADQKIEIDERASAAQAAQ
VTILLHKPVGYVSGQAEDGYEPAAVLITRANRWSGDHSPVRFSPQHLHALAPAGRLDIDSTGLLVLTQNGVIAKQLIGEQ
SDIDKEYLVRVRFGERLIDIDQHFPAESLAKLRHGLELDGVALKPALVSWQNGEQLRFVLREGKKRQIRRMCELVGLDVI
GLKRVRMGRVMLGALPQGQWRYLSADETF
>Mature_669_residues
MRTKLTVKNPKPASPTRAPVRSGSLVARKAVRPAAPPAGDKPARPKKAPAAAAATGERSFKPRGAAGAERPGADRAPAKR
APRDGGAERGARAPYRENAAGEGAKRSFGDRRTSSDRPPRRDDDARPRRASGEGSTRAPYRDNAAGEGAKRSFGDRRTSS
DRPPRRDDDARPRRAGADEGKRPSYRDNAAGEGAKRSFGDRRTASDRPPRRDDDARPRRTGADEGKRPSYRDNAAGEGAK
RSFGDRRTSSDRPARRDDDTRPRRTGTDEGKRPSYRDNASGDGAKRSFGDRRTSSDRPPRRDDDARPRRAGADEGKRPSY
RDNASGEGAKRSFGDRRTSSDRPPRRSDDDARPRRAAGGDDSKRPSYRDKSAGDGAKRSFGDRPARPTRDGERRSFGAVK
TAQPVKRAAADVDYGDETGLMRLSKRMSELGMCSRREADEWIEKGWVLVDGARIDTLGTKVRADQKIEIDERASAAQAAQ
VTILLHKPVGYVSGQAEDGYEPAAVLITRANRWSGDHSPVRFSPQHLHALAPAGRLDIDSTGLLVLTQNGVIAKQLIGEQ
SDIDKEYLVRVRFGERLIDIDQHFPAESLAKLRHGLELDGVALKPALVSWQNGEQLRFVLREGKKRQIRRMCELVGLDVI
GLKRVRMGRVMLGALPQGQWRYLSADETF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=251, Percent_Identity=35.4581673306773, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787524, Length=262, Percent_Identity=30.5343511450382, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788510, Length=251, Percent_Identity=29.4820717131474, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 73527; Mature: 73527

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKLTVKNPKPASPTRAPVRSGSLVARKAVRPAAPPAGDKPARPKKAPAAAAATGERSF
CCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
KPRGAAGAERPGADRAPAKRAPRDGGAERGARAPYRENAAGEGAKRSFGDRRTSSDRPPR
CCCCCCCCCCCCCCCCCHHCCCCCCCHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCC
RDDDARPRRASGEGSTRAPYRDNAAGEGAKRSFGDRRTSSDRPPRRDDDARPRRAGADEG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KRPSYRDNAAGEGAKRSFGDRRTASDRPPRRDDDARPRRTGADEGKRPSYRDNAAGEGAK
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
RSFGDRRTSSDRPARRDDDTRPRRTGTDEGKRPSYRDNASGDGAKRSFGDRRTSSDRPPR
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCC
RDDDARPRRAGADEGKRPSYRDNASGEGAKRSFGDRRTSSDRPPRRSDDDARPRRAAGGD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DSKRPSYRDKSAGDGAKRSFGDRPARPTRDGERRSFGAVKTAQPVKRAAADVDYGDETGL
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCHHH
MRLSKRMSELGMCSRREADEWIEKGWVLVDGARIDTLGTKVRADQKIEIDERASAAQAAQ
HHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCEECCCCCHHCCCCCCCHHHHHHHHHHEE
VTILLHKPVGYVSGQAEDGYEPAAVLITRANRWSGDHSPVRFSPQHLHALAPAGRLDIDS
EEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECHHHHHHHCCCCCCCCCC
TGLLVLTQNGVIAKQLIGEQSDIDKEYLVRVRFGERLIDIDQHFPAESLAKLRHGLELDG
CCEEEEECCCHHHHHHHCCCCCCCHHHEEEHHHCCHHEEHHHCCCHHHHHHHHHCCCCCC
VALKPALVSWQNGEQLRFVLREGKKRQIRRMCELVGLDVIGLKRVRMGRVMLGALPQGQW
EEECCHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCE
RYLSADETF
EEECCCCCC
>Mature Secondary Structure
MRTKLTVKNPKPASPTRAPVRSGSLVARKAVRPAAPPAGDKPARPKKAPAAAAATGERSF
CCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
KPRGAAGAERPGADRAPAKRAPRDGGAERGARAPYRENAAGEGAKRSFGDRRTSSDRPPR
CCCCCCCCCCCCCCCCCHHCCCCCCCHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCC
RDDDARPRRASGEGSTRAPYRDNAAGEGAKRSFGDRRTSSDRPPRRDDDARPRRAGADEG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KRPSYRDNAAGEGAKRSFGDRRTASDRPPRRDDDARPRRTGADEGKRPSYRDNAAGEGAK
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
RSFGDRRTSSDRPARRDDDTRPRRTGTDEGKRPSYRDNASGDGAKRSFGDRRTSSDRPPR
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCC
RDDDARPRRAGADEGKRPSYRDNASGEGAKRSFGDRRTSSDRPPRRSDDDARPRRAAGGD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DSKRPSYRDKSAGDGAKRSFGDRPARPTRDGERRSFGAVKTAQPVKRAAADVDYGDETGL
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCHHH
MRLSKRMSELGMCSRREADEWIEKGWVLVDGARIDTLGTKVRADQKIEIDERASAAQAAQ
HHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCEECCCCCHHCCCCCCCHHHHHHHHHHEE
VTILLHKPVGYVSGQAEDGYEPAAVLITRANRWSGDHSPVRFSPQHLHALAPAGRLDIDS
EEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECHHHHHHHCCCCCCCCCC
TGLLVLTQNGVIAKQLIGEQSDIDKEYLVRVRFGERLIDIDQHFPAESLAKLRHGLELDG
CCEEEEECCCHHHHHHHCCCCCCCHHHEEEHHHCCHHEEHHHCCCHHHHHHHHHCCCCCC
VALKPALVSWQNGEQLRFVLREGKKRQIRRMCELVGLDVIGLKRVRMGRVMLGALPQGQW
EEECCHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCE
RYLSADETF
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]