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The map label for this gene is ldcA [H]

Identifier: 116689988

GI number: 116689988

Start: 2185839

End: 2186777

Strand: Reverse

Name: ldcA [H]

Synonym: Bcen2424_1967

Alternate gene names: 116689988

Gene position: 2186777-2185839 (Counterclockwise)

Preceding gene: 116689989

Following gene: 116689987

Centisome position: 62.77

GC content: 68.69

Gene sequence:

>939_bases
ATGTCCTTCCAGCCCGCCGCGTCCTACACCATCCGCCTGCTGGCGCCGTCCGGCTATCCGCACGACCCCGACGCGATCAA
CCGCGCGCTCGAGCGCCTCAGCACCGCGCAGCAGCGCATCGAGAACATCGAGGCGACGCAGCGGCGCTTCCAGCGTTTCG
GCGGCACCGACGGCGAACGGGCAGGCGACCTGAACCGCCTCGCCGATCCCGGGCGGCCGCTGCCGGACATCGCGCTCGCG
GTGCGCGGCGGCTACGGCGCCGCGCGCATTCTGCACGGGCTCGACTATCGCGGGCTCGAACGCCGGCTGCGCGACCAGCC
GATCGCGCTCGTCGGTCACAGCGATTTCACGGCGATCCAGCTGGCGCTGTACGCGAAGGCGCGCATCAAGACGTTCGGCG
GTCCGATGCTGTCGGCGGATTTCGGCGCCGAAACGCCGAGCGACTTCACGATGCAGCATTTCTGGCAGACGCTGACGCAG
CCCAGCACGACGATCGTCGCCGATGCACCGCAGGTGCAGACGGCGAACGTGACGGGCACGCTGTGGGGCGGCAACCTCGC
GATCCTCACGTCGCTGGTCGGCACGCCGTACATGCCCGACATCGAGGGCGGCATCCTGTTCATCGAAGACGTCAACGAGC
AGCCGTTCCGGATCGAGCGGATGATCTATCAACTGCACCTGTCGGGGCTGCTCGCGCGGCAGCAGGCGCTCGTGCTCGGC
CAGTTCACCGGCGCGCGGCCGTTCGAGTACGACAACGGCTACGACATGCAGGCGATGATCGACCAAGTGCGCGCGGTGGT
CGGGATTCCGGTCGTCACCGGGCTCCAGTTCGGCCATGTGCCCGACCTGTTGACGCTGCCGTTCGGCGCACAGGCGCAGT
TGGTCGCGAACGCGCACGGGTTCCGGCTGACGCTGTCGGACTATCCGCATCTCGGCTGA

Upstream 100 bases:

>100_bases
ACCTCTTGCCGCCCCCGGCATCCCGGGCGTGCACGGTTTTCGTGAACCGGACTCGGTTCGGACCCTCTAAGCAGGTTTGA
CGACCCCGATACCTTTCGCC

Downstream 100 bases:

>100_bases
TGCCGGCGCCGGCGCGATCGATCGAAAGGCGGGGCTCCCCGCCTTTTTCTTTATGTAAGCGCGCAACTAACGGTCCATAT
TTTTCGTCGACGTCGACATG

Product: L,D-carboxypeptidase A

Products: NA

Alternate protein names: LD-carboxypeptidase A; Muramoyltetrapeptide carboxypeptidase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSFQPAASYTIRLLAPSGYPHDPDAINRALERLSTAQQRIENIEATQRRFQRFGGTDGERAGDLNRLADPGRPLPDIALA
VRGGYGAARILHGLDYRGLERRLRDQPIALVGHSDFTAIQLALYAKARIKTFGGPMLSADFGAETPSDFTMQHFWQTLTQ
PSTTIVADAPQVQTANVTGTLWGGNLAILTSLVGTPYMPDIEGGILFIEDVNEQPFRIERMIYQLHLSGLLARQQALVLG
QFTGARPFEYDNGYDMQAMIDQVRAVVGIPVVTGLQFGHVPDLLTLPFGAQAQLVANAHGFRLTLSDYPHLG

Sequences:

>Translated_312_residues
MSFQPAASYTIRLLAPSGYPHDPDAINRALERLSTAQQRIENIEATQRRFQRFGGTDGERAGDLNRLADPGRPLPDIALA
VRGGYGAARILHGLDYRGLERRLRDQPIALVGHSDFTAIQLALYAKARIKTFGGPMLSADFGAETPSDFTMQHFWQTLTQ
PSTTIVADAPQVQTANVTGTLWGGNLAILTSLVGTPYMPDIEGGILFIEDVNEQPFRIERMIYQLHLSGLLARQQALVLG
QFTGARPFEYDNGYDMQAMIDQVRAVVGIPVVTGLQFGHVPDLLTLPFGAQAQLVANAHGFRLTLSDYPHLG
>Mature_311_residues
SFQPAASYTIRLLAPSGYPHDPDAINRALERLSTAQQRIENIEATQRRFQRFGGTDGERAGDLNRLADPGRPLPDIALAV
RGGYGAARILHGLDYRGLERRLRDQPIALVGHSDFTAIQLALYAKARIKTFGGPMLSADFGAETPSDFTMQHFWQTLTQP
STTIVADAPQVQTANVTGTLWGGNLAILTSLVGTPYMPDIEGGILFIEDVNEQPFRIERMIYQLHLSGLLARQQALVLGQ
FTGARPFEYDNGYDMQAMIDQVRAVVGIPVVTGLQFGHVPDLLTLPFGAQAQLVANAHGFRLTLSDYPHLG

Specific function: Releases the terminal D-alanine residue from the cytoplasmic tetrapeptide recycling product L-Ala-gamma-D-Glu-meso- Dap-D-Ala. Can also cleave D-Ala from murein derivatives containing the tetrapeptide, i.e. MurNAc-tetrapeptide, UDP-MurNAc- tetrapeptide, G

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=299, Percent_Identity=47.8260869565217, Blast_Score=281, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 34168; Mature: 34037

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQPAASYTIRLLAPSGYPHDPDAINRALERLSTAQQRIENIEATQRRFQRFGGTDGER
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGDLNRLADPGRPLPDIALAVRGGYGAARILHGLDYRGLERRLRDQPIALVGHSDFTAIQ
CCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCHHHH
LALYAKARIKTFGGPMLSADFGAETPSDFTMQHFWQTLTQPSTTIVADAPQVQTANVTGT
HHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCEEECCEEEE
LWGGNLAILTSLVGTPYMPDIEGGILFIEDVNEQPFRIERMIYQLHLSGLLARQQALVLG
EECCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
QFTGARPFEYDNGYDMQAMIDQVRAVVGIPVVTGLQFGHVPDLLTLPFGAQAQLVANAHG
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCEEECCCCCCEEEEECCCC
FRLTLSDYPHLG
EEEEECCCCCCC
>Mature Secondary Structure 
SFQPAASYTIRLLAPSGYPHDPDAINRALERLSTAQQRIENIEATQRRFQRFGGTDGER
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGDLNRLADPGRPLPDIALAVRGGYGAARILHGLDYRGLERRLRDQPIALVGHSDFTAIQ
CCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCHHHH
LALYAKARIKTFGGPMLSADFGAETPSDFTMQHFWQTLTQPSTTIVADAPQVQTANVTGT
HHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCEEECCEEEE
LWGGNLAILTSLVGTPYMPDIEGGILFIEDVNEQPFRIERMIYQLHLSGLLARQQALVLG
EECCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
QFTGARPFEYDNGYDMQAMIDQVRAVVGIPVVTGLQFGHVPDLLTLPFGAQAQLVANAHG
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCEEECCCCCCEEEEECCCC
FRLTLSDYPHLG
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]