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The map label for this gene is dmlR [H]

Identifier: 116689898

GI number: 116689898

Start: 2081911

End: 2082876

Strand: Reverse

Name: dmlR [H]

Synonym: Bcen2424_1877

Alternate gene names: 116689898

Gene position: 2082876-2081911 (Counterclockwise)

Preceding gene: 116689903

Following gene: 116689897

Centisome position: 59.79

GC content: 68.94

Gene sequence:

>966_bases
ATGACGGCCGGACGGGGCTTCCCGCCGGTGTCGGAGCCGGGACGGAGACAGGGGATGGATCGTTTCAAGCAGATCGAGAC
GTTCGTGCGGGTGGCCGATGCCGGCAGCCTCGCGGCGGCGGCGTTGGAGGAAGGTGTGTCGCCAGTGGTGCTCGGGCGGC
GCATCGACGCGCTGGAGAAGCGCCTCGGCGTGAAGCTGATGTACCGCTCGACGCGGCGGCTGGTCGTCAGCGAGGAGGGC
GCCGCGTTCCTCGAGCGCTGTCGCGGGCTGCTGTCCGAATGGGATCAGGCCGAGAACGAACTGGCCGCCGGTCGGCGCGC
GGTCAGCGGCCACCTGATCGTGTCGGCGCCGGCCGCGTTCGGGCGCAAGCACGTCGCGCCGCACGCGCCGGGCTTCCTCG
CCGACAAGCCGGAGATGCAGCTGTCGTTCAACCTGACCGACCGCGTCGTCGATCTCGTGCGCGAAGGCTACGACCTGTCG
ATCCGGATCGGCGGCGCGGTCGATCCGAATTTCGTCGCGGTGAAGCTCGCGTCGAACCGGCGCGTGGTGTGCGGCACGCC
CGACTATTTCCGCCTGCACGGCCGGCCGAAGTCGCTCGACGACCTGCTGAAGCACAACTGCCTGGCGTTCAACCTGCAGG
GCGGGCAGAACCGCGGCTGGTATTTCCAGCGCCACGGCAAGATCGCGACGATGCGCGTGACCGGCAATCTCGACTGCAAC
GACGGCGAGCTGCTGCACCGCTGGGTGGCCGAGGGGCTCGGGCTCGGCTGGCGCTCGACGTGGGAAATCGCCGCGCAACT
CGAAACGGGCGAGCTCGAGACCGTACTCGACGACTATGCGCTGCCGGACTACGACATCCTCGCGGTCTATCCGCAGCAGC
GCTACGTGCCGGCGCGCGTGCGCTACTTCATCGACTACCTGCGCGAAGCGTATGCACGCCCCGGCTACTGGAGCACCACG
CCGTAA

Upstream 100 bases:

>100_bases
GTTGAATCTTGCGAATCGGTCTGTTGAGGACACTTTAGGGATGCAGGAAAGGTTTGATAAGATCAGCCGAAGTCGCTTAT
TTCGAAACTAAAAGTATGGA

Downstream 100 bases:

>100_bases
CGGGCGGCCCGCCTGGCCGTCCGGCGATTTTTTTACGCTTCATGCGTGCCGCGCGGGAATAAACTCGACGCAGCGGCGGT
AAGGTCATCGAGCGCGCACG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTAGRGFPPVSEPGRRQGMDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEEG
AAFLERCRGLLSEWDQAENELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLADKPEMQLSFNLTDRVVDLVREGYDLS
IRIGGAVDPNFVAVKLASNRRVVCGTPDYFRLHGRPKSLDDLLKHNCLAFNLQGGQNRGWYFQRHGKIATMRVTGNLDCN
DGELLHRWVAEGLGLGWRSTWEIAAQLETGELETVLDDYALPDYDILAVYPQQRYVPARVRYFIDYLREAYARPGYWSTT
P

Sequences:

>Translated_321_residues
MTAGRGFPPVSEPGRRQGMDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEEG
AAFLERCRGLLSEWDQAENELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLADKPEMQLSFNLTDRVVDLVREGYDLS
IRIGGAVDPNFVAVKLASNRRVVCGTPDYFRLHGRPKSLDDLLKHNCLAFNLQGGQNRGWYFQRHGKIATMRVTGNLDCN
DGELLHRWVAEGLGLGWRSTWEIAAQLETGELETVLDDYALPDYDILAVYPQQRYVPARVRYFIDYLREAYARPGYWSTT
P
>Mature_320_residues
TAGRGFPPVSEPGRRQGMDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEEGA
AFLERCRGLLSEWDQAENELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLADKPEMQLSFNLTDRVVDLVREGYDLSI
RIGGAVDPNFVAVKLASNRRVVCGTPDYFRLHGRPKSLDDLLKHNCLAFNLQGGQNRGWYFQRHGKIATMRVTGNLDCND
GELLHRWVAEGLGLGWRSTWEIAAQLETGELETVLDDYALPDYDILAVYPQQRYVPARVRYFIDYLREAYARPGYWSTTP

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=36.0824742268041, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=30.5263157894737, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=30.9027777777778, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1787128, Length=294, Percent_Identity=29.9319727891156, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=28.6689419795222, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=25.5892255892256, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=27.2727272727273, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790011, Length=301, Percent_Identity=21.2624584717608, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35873; Mature: 35742

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGRGFPPVSEPGRRQGMDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHH
RLGVKLMYRSTRRLVVSEEGAAFLERCRGLLSEWDQAENELAAGRRAVSGHLIVSAPAAF
HHCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECCHHH
GRKHVAPHAPGFLADKPEMQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLASNR
CCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCC
RVVCGTPDYFRLHGRPKSLDDLLKHNCLAFNLQGGQNRGWYFQRHGKIATMRVTGNLDCN
EEEECCCCEEEECCCCCCHHHHHHCCEEEEEECCCCCCCEEEEECCEEEEEEEECCCCCC
DGELLHRWVAEGLGLGWRSTWEIAAQLETGELETVLDDYALPDYDILAVYPQQRYVPARV
CHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCEEEECCCCCCCHHHH
RYFIDYLREAYARPGYWSTTP
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TAGRGFPPVSEPGRRQGMDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHH
RLGVKLMYRSTRRLVVSEEGAAFLERCRGLLSEWDQAENELAAGRRAVSGHLIVSAPAAF
HHCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECCHHH
GRKHVAPHAPGFLADKPEMQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLASNR
CCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCC
RVVCGTPDYFRLHGRPKSLDDLLKHNCLAFNLQGGQNRGWYFQRHGKIATMRVTGNLDCN
EEEECCCCEEEECCCCCCHHHHHHCCEEEEEECCCCCCCEEEEECCEEEEEEEECCCCCC
DGELLHRWVAEGLGLGWRSTWEIAAQLETGELETVLDDYALPDYDILAVYPQQRYVPARV
CHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCEEEECCCCCCCHHHH
RYFIDYLREAYARPGYWSTTP
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]