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The map label for this gene is ansA [H]

Identifier: 116689903

GI number: 116689903

Start: 2087608

End: 2088705

Strand: Reverse

Name: ansA [H]

Synonym: Bcen2424_1882

Alternate gene names: 116689903

Gene position: 2088705-2087608 (Counterclockwise)

Preceding gene: 116689907

Following gene: 116689898

Centisome position: 59.95

GC content: 72.22

Gene sequence:

>1098_bases
ATGACACGGGGTCGCGCCGCGCGCGTTCGAGCCGGACCTACCGGACGCCGGCCGCGAGCCATTACAATCGCGGCCATGAA
TACACCGAACACCGCATCCCCTTCGTCCGCGCCGGCGCTGCCGCGCATCGCCGTACTCGCCACCGGCGGCACGATCGCCG
GCGCCGCGCCCGACGCGGCCAGCACGGCCGGCTACCAGGCCGGCGCGCTCGGCGTCAATTTCCTGGTCGACGCCGTACCG
GCGCTTGCGTCGGTCGCGCGCATCGACGCGGAGCAGGTCGCCAGCATCGACAGCAAGGATCTCGCGCTGCCGCTGTGGAA
CACGCTCGCCGCGCGGATCGACGCGCTGATGGCCGACCCGGCGATCGACGGCATCGTGATCACCCACGGCACCGATACGC
TCGAGGAAACCGCGTATGCACTGCATCTGGTCGTCCGGGGCGACAAGCCGGTCGTGCTGACGGCCGCGATGCGGCCGGCG
ACCGCGCTGTCGTCCGACGGCCCGCTGAACCTGCTGAACGCGGTGACGGTGGCCGCCCATCCGGCCGCGCGCGGGCAAGG
CGTGCTGGTCGCGTTCAACAACCGGATCCACGGCGCGCGCGACGTCGTGAAGACGAGCACCTATGCGGTCGACGCGTTCC
AGTCGCCGGAGCTCGGCGCACTCGGCTGGGTGCAGGACGGCCGCGTCGAATTCGCCCGCCGTGTCACGCGCACGCGCGAC
ACGCAGCTGGCGATCGCGCCGACCTGGCCGCCGGTCGAAGTCGTCGCGAGCTATGCGGGCGTGACGCGCACGGCCGTCGA
TGCGCTCGTCGCGGCCGGCGTGCGCGGCCTCGTCGTCGCGGGCACCGGCAACGGATCGATCCATGCGACGCTGCAGACGG
CGCTCGCCGATGCGGTGAACGCGGGCGTGGCGGTGGTGCGTGCGTCGCGCGTCGGTTCGGGGCACGTGATGCGCAACGGC
GCCGCGAGCGACGATGCGCTCGGGTTCGTGAGCGCGGGCTCGCTGCACCCGTTCAAGGCGCGCGTGCTGCTGATGCTCGC
GCTCGCGAACGGCATTCGCGAGCGCGACGCATTGCAACGCGCATTCGACACGCTGTGA

Upstream 100 bases:

>100_bases
TTATGGCAAATCAATGTGAAAACACCAAAACGGCATTTCTATAACGCAGTGGGCGGCATGGCCGTTGACGGCGTGCACGC
GCCGGCGATCGGGACAAACC

Downstream 100 bases:

>100_bases
CCGATGGCGCGCCGCTCGGGCGGCGCGTAGAAGGCATAAAAAAATGGCAGGCCAATGGCCTGCCATTTTTGCGTCAGTGC
TGCGCGAATGCTCAGGACTG

Product: L-asparaginases, type II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTRGRAARVRAGPTGRRPRAITIAAMNTPNTASPSSAPALPRIAVLATGGTIAGAAPDAASTAGYQAGALGVNFLVDAVP
ALASVARIDAEQVASIDSKDLALPLWNTLAARIDALMADPAIDGIVITHGTDTLEETAYALHLVVRGDKPVVLTAAMRPA
TALSSDGPLNLLNAVTVAAHPAARGQGVLVAFNNRIHGARDVVKTSTYAVDAFQSPELGALGWVQDGRVEFARRVTRTRD
TQLAIAPTWPPVEVVASYAGVTRTAVDALVAAGVRGLVVAGTGNGSIHATLQTALADAVNAGVAVVRASRVGSGHVMRNG
AASDDALGFVSAGSLHPFKARVLLMLALANGIRERDALQRAFDTL

Sequences:

>Translated_365_residues
MTRGRAARVRAGPTGRRPRAITIAAMNTPNTASPSSAPALPRIAVLATGGTIAGAAPDAASTAGYQAGALGVNFLVDAVP
ALASVARIDAEQVASIDSKDLALPLWNTLAARIDALMADPAIDGIVITHGTDTLEETAYALHLVVRGDKPVVLTAAMRPA
TALSSDGPLNLLNAVTVAAHPAARGQGVLVAFNNRIHGARDVVKTSTYAVDAFQSPELGALGWVQDGRVEFARRVTRTRD
TQLAIAPTWPPVEVVASYAGVTRTAVDALVAAGVRGLVVAGTGNGSIHATLQTALADAVNAGVAVVRASRVGSGHVMRNG
AASDDALGFVSAGSLHPFKARVLLMLALANGIRERDALQRAFDTL
>Mature_364_residues
TRGRAARVRAGPTGRRPRAITIAAMNTPNTASPSSAPALPRIAVLATGGTIAGAAPDAASTAGYQAGALGVNFLVDAVPA
LASVARIDAEQVASIDSKDLALPLWNTLAARIDALMADPAIDGIVITHGTDTLEETAYALHLVVRGDKPVVLTAAMRPAT
ALSSDGPLNLLNAVTVAAHPAARGQGVLVAFNNRIHGARDVVKTSTYAVDAFQSPELGALGWVQDGRVEFARRVTRTRDT
QLAIAPTWPPVEVVASYAGVTRTAVDALVAAGVRGLVVAGTGNGSIHATLQTALADAVNAGVAVVRASRVGSGHVMRNGA
ASDDALGFVSAGSLHPFKARVLLMLALANGIRERDALQRAFDTL

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=332, Percent_Identity=44.578313253012, Blast_Score=247, Evalue=7e-67,
Organism=Escherichia coli, GI1788065, Length=249, Percent_Identity=32.5301204819277, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17558280, Length=244, Percent_Identity=29.5081967213115, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17558278, Length=244, Percent_Identity=29.5081967213115, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI193207372, Length=244, Percent_Identity=29.5081967213115, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI193207374, Length=244, Percent_Identity=29.5081967213115, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=328, Percent_Identity=37.1951219512195, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6323187, Length=328, Percent_Identity=37.1951219512195, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6323186, Length=328, Percent_Identity=37.1951219512195, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6323184, Length=328, Percent_Identity=37.1951219512195, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6320527, Length=322, Percent_Identity=33.8509316770186, Blast_Score=172, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24645530, Length=233, Percent_Identity=28.3261802575107, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24639609, Length=237, Percent_Identity=25.3164556962025, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37320; Mature: 37189

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRGRAARVRAGPTGRRPRAITIAAMNTPNTASPSSAPALPRIAVLATGGTIAGAAPDAA
CCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEECCCCCCCC
STAGYQAGALGVNFLVDAVPALASVARIDAEQVASIDSKDLALPLWNTLAARIDALMADP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
AIDGIVITHGTDTLEETAYALHLVVRGDKPVVLTAAMRPATALSSDGPLNLLNAVTVAAH
CCCCEEEECCCCHHHHHEEEEEEEEECCCCEEEEECCCCCHHCCCCCCHHHHHHHHHHCC
PAARGQGVLVAFNNRIHGARDVVKTSTYAVDAFQSPELGALGWVQDGRVEFARRVTRTRD
CCCCCCEEEEEECCCCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCC
TQLAIAPTWPPVEVVASYAGVTRTAVDALVAAGVRGLVVAGTGNGSIHATLQTALADAVN
CEEEEECCCCHHHHHHHHHCHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHHH
AGVAVVRASRVGSGHVMRNGAASDDALGFVSAGSLHPFKARVLLMLALANGIRERDALQR
CCEEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHH
AFDTL
HHHCC
>Mature Secondary Structure 
TRGRAARVRAGPTGRRPRAITIAAMNTPNTASPSSAPALPRIAVLATGGTIAGAAPDAA
CCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEECCCCCCCC
STAGYQAGALGVNFLVDAVPALASVARIDAEQVASIDSKDLALPLWNTLAARIDALMADP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
AIDGIVITHGTDTLEETAYALHLVVRGDKPVVLTAAMRPATALSSDGPLNLLNAVTVAAH
CCCCEEEECCCCHHHHHEEEEEEEEECCCCEEEEECCCCCHHCCCCCCHHHHHHHHHHCC
PAARGQGVLVAFNNRIHGARDVVKTSTYAVDAFQSPELGALGWVQDGRVEFARRVTRTRD
CCCCCCEEEEEECCCCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCC
TQLAIAPTWPPVEVVASYAGVTRTAVDALVAAGVRGLVVAGTGNGSIHATLQTALADAVN
CEEEEECCCCHHHHHHHHHCHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHHH
AGVAVVRASRVGSGHVMRNGAASDDALGFVSAGSLHPFKARVLLMLALANGIRERDALQR
CCEEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHH
AFDTL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]