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The map label for this gene is pit [H]

Identifier: 116689889

GI number: 116689889

Start: 2075200

End: 2076210

Strand: Reverse

Name: pit [H]

Synonym: Bcen2424_1868

Alternate gene names: 116689889

Gene position: 2076210-2075200 (Counterclockwise)

Preceding gene: 116689890

Following gene: 116689876

Centisome position: 59.59

GC content: 65.58

Gene sequence:

>1011_bases
ATGCATTCGATACAACTCGCCCTATGGATGGTCGCGGCACTGGTGCTCGTCGCGCTCGTATTCGACTTCATGAACGGCTT
TCACGACGCGGCGAACTCGATCGCCACCGTCGTGTCGACCGGGGTGCTGAAGCCCCAGCAGGCCGTCGTGTTCGCGGCCG
CGTTCAACGTGATCGCGTACTTCATCTTCCACCTGAAAGTCGCGCAGACCGTCGGTAAAGGCACGATCGACCCCGACATC
GTCGACCATTACGTCATATTCGGCGCGCTGGTCGGCGCGATCGGCTGGAACGTGATCACCTGGTACTACGGGATTCCGTC
GAGCTCGTCGCACGCGCTGATCGGCGGGCTCGTCGGTGCGGCGCTCGCGAAGTCGGGCTGGAGCTCGCTGAACATCGACG
GGCTGCTGAAGACGATCGCGTTCATCTTCATTTCGCCGCTGCTCGGTTTCATCCTCGGTTCGCTGTTCATGCTCGGCGTG
TCGTGGCTGTATTTCCGTACCGCGCCCAGCAAGGTCGACCGGCGCTTCCGCCGGCTGCAGCTGCTGTCGGCCGGGCTGTA
CAGCCTGGGCCACGGCGGCAACGACGCACAGAAGACGATCGGTATCATCTGGATGCTGCTGATCGCGAGCGGCTACGCGT
CGGCGACCGCCGATGCGCCGCCGGCGTGGGTGATCGGCGCGTGTTACCTGTCGATGGGCCTCGGTACGCTGTTCGGCGGC
TGGCGCATCGTGCGCACGATGGGCCAGAAGATCACGAAGCTCAAGCCGGTCGGCGGTTTCTGTGCGGAAAGCGGCGGGGC
GATCACGCTGTTCGTCGCGTCGTTCCTCGGCATTCCCGTGTCGACCACGCACACGATCACCGGCGCGATCGTCGGTGTCG
GCGCCACGCAGAAGCTGTCGGCCGTGCGCTGGGGTGTCGCCGGCAACATCGTGTGGGCGTGGGTGCTGACGCTGCCGGCG
GCCGCGCTGTTCGCGGCCGGCGGGTGGTGGCTCGGGCACCAGATCTTCTGA

Upstream 100 bases:

>100_bases
AAGGCGATCTACGAGCTGCTCGAAGAGATCACCGACAAGTGCGAGGACGTCGCGAACATCATCGAAGGCATCGTGCTGGA
AAACGCCTGAGCGGACCACG

Downstream 100 bases:

>100_bases
TCGCCGACGCCGCCTGATCGACGCGAAATGCGCCGCGAGCCGAAAGGCCGCGGCGCATTTTCTTTTGGGGGGGGTGCCCC
TATGTCTTTCGTCAAGCGTT

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MHSIQLALWMVAALVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFIFHLKVAQTVGKGTIDPDI
VDHYVIFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGAALAKSGWSSLNIDGLLKTIAFIFISPLLGFILGSLFMLGV
SWLYFRTAPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASATADAPPAWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAESGGAITLFVASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTLPA
AALFAAGGWWLGHQIF

Sequences:

>Translated_336_residues
MHSIQLALWMVAALVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFIFHLKVAQTVGKGTIDPDI
VDHYVIFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGAALAKSGWSSLNIDGLLKTIAFIFISPLLGFILGSLFMLGV
SWLYFRTAPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASATADAPPAWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAESGGAITLFVASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTLPA
AALFAAGGWWLGHQIF
>Mature_336_residues
MHSIQLALWMVAALVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFIFHLKVAQTVGKGTIDPDI
VDHYVIFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGAALAKSGWSSLNIDGLLKTIAFIFISPLLGFILGSLFMLGV
SWLYFRTAPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASATADAPPAWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAESGGAITLFVASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTLPA
AALFAAGGWWLGHQIF

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=158, Percent_Identity=37.3417721518987, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI31543630, Length=168, Percent_Identity=34.5238095238095, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1789907, Length=218, Percent_Identity=31.651376146789, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789360, Length=246, Percent_Identity=31.3008130081301, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17539280, Length=176, Percent_Identity=34.0909090909091, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25146401, Length=163, Percent_Identity=31.2883435582822, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32566716, Length=169, Percent_Identity=31.9526627218935, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71981576, Length=163, Percent_Identity=28.2208588957055, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17557328, Length=153, Percent_Identity=33.9869281045752, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=169, Percent_Identity=29.585798816568, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319773, Length=172, Percent_Identity=31.9767441860465, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356511, Length=184, Percent_Identity=33.1521739130435, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35532; Mature: 35532

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCC
>Mature Secondary Structure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HHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]