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The map label for this gene is rlmE [H]

Identifier: 116689318

GI number: 116689318

Start: 1425784

End: 1426446

Strand: Direct

Name: rlmE [H]

Synonym: Bcen2424_1296

Alternate gene names: 116689318

Gene position: 1425784-1426446 (Clockwise)

Preceding gene: 116689316

Following gene: 116689319

Centisome position: 40.92

GC content: 62.9

Gene sequence:

>663_bases
ATGGCAAAAAACCGCTTCAACCAGCACTGGCTGCACGACCACATCAACGACCCGTACGTCAAAATGGCGCAGCGGGAGGG
CTATCGCGCGCGCGCCGCGTACAAGCTGAAGGAAATCGACGAACAGGACAAGCTGATCCGTCCCGGCCAGGTGATCGTCG
ACCTCGGCGCCACGCCGGGCAGCTGGAGCCAGTATGCCCGCAACAAGCTCGCGCAGGGCAAGAAGCGTGACGCGGAGCGC
GAAGGCGGCATCGACGGCACGATCGTTGCGCTCGACATCCTGCCGATGGAGCCGATCGCCGACGTCCACTTCCTGCAGGG
CGACTTCCGCGAGGACGACGTCCTTCACCAGCTCGAAGAAGTGCTCGAAGGCCGCGCGGTGGACCTTGTTATTTCCGACA
TGGCCCCCAACCTCTCCGGCGTGGCCTCGGCGGACGCGGCGCGCATCGAGCATCTCTGCGATCTGGCGCTCGAATTCGCG
CAGAACCATCTGAAGCCGGACGGTGCGCTGCTCGTGAAGTGCTTTCACGGCAGCGGCTACAGCCAGATCGTCGAGAAGTT
CAAACAGCAGTTTAAGGTTGTCGCGCCGCGCAAGCCCAAGGCATCCCGCGACAAATCGTCCGAAACGTTCATTTTGGGTC
GGCAGCTGAAGCATCCGCGCTGA

Upstream 100 bases:

>100_bases
GGCTTGGGCCTGAAAAGGGGCCGCAGCCGCGCGAAGAAGACGCGTATTATCCGCTAAAAGCGCGGCTTCACGAAGCAATT
GGCAGCAATCTCTCTCTCGA

Downstream 100 bases:

>100_bases
TGCGGAGTGCGGTGCCGCAAACGGGCTCCGGCCCTTGCCAGACAAGCGAAGCGCTATCGCGGCGGTTGTCAATGCTTATG
GCGGGGGTGGTCGGACTGGA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKNRFNQHWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPGSWSQYARNKLAQGKKRDAER
EGGIDGTIVALDILPMEPIADVHFLQGDFREDDVLHQLEEVLEGRAVDLVISDMAPNLSGVASADAARIEHLCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKQQFKVVAPRKPKASRDKSSETFILGRQLKHPR

Sequences:

>Translated_220_residues
MAKNRFNQHWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPGSWSQYARNKLAQGKKRDAER
EGGIDGTIVALDILPMEPIADVHFLQGDFREDDVLHQLEEVLEGRAVDLVISDMAPNLSGVASADAARIEHLCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKQQFKVVAPRKPKASRDKSSETFILGRQLKHPR
>Mature_219_residues
AKNRFNQHWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPGSWSQYARNKLAQGKKRDAERE
GGIDGTIVALDILPMEPIADVHFLQGDFREDDVLHQLEEVLEGRAVDLVISDMAPNLSGVASADAARIEHLCDLALEFAQ
NHLKPDGALLVKCFHGSGYSQIVEKFKQQFKVVAPRKPKASRDKSSETFILGRQLKHPR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=218, Percent_Identity=36.2385321100917, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI194097365, Length=203, Percent_Identity=29.5566502463054, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI29029591, Length=203, Percent_Identity=31.5270935960591, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI29029589, Length=203, Percent_Identity=31.5270935960591, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI7110661, Length=203, Percent_Identity=31.5270935960591, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1789569, Length=206, Percent_Identity=42.7184466019417, Blast_Score=182, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17554650, Length=203, Percent_Identity=33.9901477832512, Blast_Score=113, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17553474, Length=225, Percent_Identity=32, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71987550, Length=203, Percent_Identity=31.0344827586207, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71987561, Length=203, Percent_Identity=31.0344827586207, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=203, Percent_Identity=31.0344827586207, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=205, Percent_Identity=31.7073170731707, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=32.6633165829146, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321302, Length=287, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356387, Length=218, Percent_Identity=33.9449541284404, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24648639, Length=203, Percent_Identity=32.0197044334975, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24647580, Length=204, Percent_Identity=32.843137254902, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI18859957, Length=196, Percent_Identity=29.0816326530612, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24788; Mature: 24656

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNRFNQHWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPG
CCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAQGKKRDAEREGGIDGTIVALDILPMEPIADVHFLQGDFREDDVLHQLEE
CHHHHHHHHHHCCHHHCHHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHH
VLEGRAVDLVISDMAPNLSGVASADAARIEHLCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKQQFKVVAPRKPKASRDKSSETFILGRQLKHPR
HHHHHHHHHHHEECCCCCCCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
AKNRFNQHWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPG
CCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAQGKKRDAEREGGIDGTIVALDILPMEPIADVHFLQGDFREDDVLHQLEE
CHHHHHHHHHHCCHHHCHHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHH
VLEGRAVDLVISDMAPNLSGVASADAARIEHLCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKQQFKVVAPRKPKASRDKSSETFILGRQLKHPR
HHHHHHHHHHHEECCCCCCCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA