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The map label for this gene is psdht [H]

Identifier: 116689279

GI number: 116689279

Start: 1390236

End: 1391228

Strand: Direct

Name: psdht [H]

Synonym: Bcen2424_1257

Alternate gene names: 116689279

Gene position: 1390236-1391228 (Clockwise)

Preceding gene: 116689276

Following gene: 116689282

Centisome position: 39.9

GC content: 71.6

Gene sequence:

>993_bases
ATGACCGTCATCGCCGCGCTCGCTTCCTCCGGAGTCGCGTGTGTTTCCTCCGTCCCCACTGCGTCCCGCCCCATGCCGCT
TCATATCCCGACCCCTTATATCCGCTCACCCATCGCGTCCCGCCGGCTCGGCAGGACCGTGCGGCTGAAGCTCGATGCGC
TGCAGCCATCGGGCTCGTTCAAGCTGCGCGGCATCGGCGCCGTCTGCGAGGCACGCCATGCGGCCGGGGCGCGGCGGTTC
GTGTCGTCGTCGGGCGGCAACGCGGGCATCGCGGTCGCGTATTGCGGCCGCGAACTCGGCGTGCCGGTGCGCGTCGTCGT
GCCGGAAAGCGCATCGGCCCGGGCGCGCGAGCTGATCCGCATCGAAGGCGCGGACCTCGTCGTGCACGGCGCGAGCTGGG
CCGAGGCAAATGCGTTCGCGCAATCGACGCTCGACGAGCACGACGCGTTCGTGCATCCGTTCGACGATCGCGTGCTGTGG
CAGGGCCACGCGACGATGATCGACGAGATGGCGGCGGCCGGTCCGAAGCCCGACGCGGTCGTGCTCGCGGTCGGCGGCGG
CGGTTTGCTGTGCGGCGTGCTGGAAGGGTTGGCGCGCAACGGCTGGCACGACGTGCCGGTCGTCGCGGCCGAGACGGAAG
GCGCCGACTGCTATGCGCGCTCGGTCGCGCAGGGGCGTCCGGTCGAACTGGCGGCCATCGCGAGCATCGCGACGTCGCTC
GGCGCGAAGCGGCCATGTGACGCGGCGGTCGAGTGGGCGACGCGCCACGCGATCCATCCGGTCGTCGTGTCCGATGCCGA
CGCGGTGGCTGCGTCGCTGCGGTTTGTCGATGAACACCGGATCGTCGTCGAGCCCGCGTGCGGCGCTGCCTTGGCCGCGC
TGGAGCGCCCGGTGCCGGTGCTCGCATCGGCGTCGGACCTTGCGGTGATCGTCTGCGGCGGCGTGACCACGACCGCCGAG
CATCTGCAGACGCTGCGTGCGACGTTGCGGTAG

Upstream 100 bases:

>100_bases
ATCTGGCGCATGCGGTGTCCGGTTCGATGGATGGATATGCCTGTAAAGCATAGCAAACGCCATTCGATGGTATTGGAAGG
CATGGCCGGCCTGCCGTACG

Downstream 100 bases:

>100_bases
GTGAGGGCGCCGCCAAGCCGCGCGGCGCGAGACGAGGCGCCTGCGATTTTCGTCCCGGTGGCGACCGCGGGTTGGGGCAC
GTAGCTAACCGCGACGCGTC

Product: L-serine ammonia-lyase

Products: oxobutanoate; NH3; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTVIAALASSGVACVSSVPTASRPMPLHIPTPYIRSPIASRRLGRTVRLKLDALQPSGSFKLRGIGAVCEARHAAGARRF
VSSSGGNAGIAVAYCGRELGVPVRVVVPESASARARELIRIEGADLVVHGASWAEANAFAQSTLDEHDAFVHPFDDRVLW
QGHATMIDEMAAAGPKPDAVVLAVGGGGLLCGVLEGLARNGWHDVPVVAAETEGADCYARSVAQGRPVELAAIASIATSL
GAKRPCDAAVEWATRHAIHPVVVSDADAVAASLRFVDEHRIVVEPACGAALAALERPVPVLASASDLAVIVCGGVTTTAE
HLQTLRATLR

Sequences:

>Translated_330_residues
MTVIAALASSGVACVSSVPTASRPMPLHIPTPYIRSPIASRRLGRTVRLKLDALQPSGSFKLRGIGAVCEARHAAGARRF
VSSSGGNAGIAVAYCGRELGVPVRVVVPESASARARELIRIEGADLVVHGASWAEANAFAQSTLDEHDAFVHPFDDRVLW
QGHATMIDEMAAAGPKPDAVVLAVGGGGLLCGVLEGLARNGWHDVPVVAAETEGADCYARSVAQGRPVELAAIASIATSL
GAKRPCDAAVEWATRHAIHPVVVSDADAVAASLRFVDEHRIVVEPACGAALAALERPVPVLASASDLAVIVCGGVTTTAE
HLQTLRATLR
>Mature_329_residues
TVIAALASSGVACVSSVPTASRPMPLHIPTPYIRSPIASRRLGRTVRLKLDALQPSGSFKLRGIGAVCEARHAAGARRFV
SSSGGNAGIAVAYCGRELGVPVRVVVPESASARARELIRIEGADLVVHGASWAEANAFAQSTLDEHDAFVHPFDDRVLWQ
GHATMIDEMAAAGPKPDAVVLAVGGGGLLCGVLEGLARNGWHDVPVVAAETEGADCYARSVAQGRPVELAAIASIATSLG
AKRPCDAAVEWATRHAIHPVVVSDADAVAASLRFVDEHRIVVEPACGAALAALERPVPVLASASDLAVIVCGGVTTTAEH
LQTLRATLR

Specific function: Catalyzes The Formation Of Alpha-Ketobutyrate From Threonine In A Two-Step Reaction. The First Step Is A Dehydration Of Threonine, Followed By Rehydration And Liberation Of Ammonia. Deaminates L-Threonine, But Also L-Serine To A Lesser Extent. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI33469958, Length=321, Percent_Identity=42.9906542056075, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI19923959, Length=321, Percent_Identity=40.1869158878505, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI11345492, Length=296, Percent_Identity=27.7027027027027, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1790207, Length=275, Percent_Identity=28.7272727272727, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71991565, Length=295, Percent_Identity=28.4745762711864, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508781, Length=148, Percent_Identity=28.3783783783784, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319788, Length=310, Percent_Identity=36.1290322580645, Blast_Score=193, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6322024, Length=196, Percent_Identity=37.7551020408163, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322023, Length=121, Percent_Identity=34.7107438016529, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355833, Length=286, Percent_Identity=29.7202797202797, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19 [C]

Molecular weight: Translated: 34103; Mature: 33972

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIAALASSGVACVSSVPTASRPMPLHIPTPYIRSPIASRRLGRTVRLKLDALQPSGSF
CEEEEEHHCCCCEEECCCCCCCCCEEEECCCHHHHCCHHHHHCCCEEEEEEEEECCCCCE
KLRGIGAVCEARHAAGARRFVSSSGGNAGIAVAYCGRELGVPVRVVVPESASARARELIR
EEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCEEEEECCCCCHHHHHEEE
IEGADLVVHGASWAEANAFAQSTLDEHDAFVHPFDDRVLWQGHATMIDEMAAAGPKPDAV
ECCCEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCEEECCCHHHHHHHHHCCCCCCEE
VLAVGGGGLLCGVLEGLARNGWHDVPVVAAETEGADCYARSVAQGRPVELAAIASIATSL
EEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHH
GAKRPCDAAVEWATRHAIHPVVVSDADAVAASLRFVDEHRIVVEPACGAALAALERPVPV
CCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHEEECCEEEEECCCCHHHHHHCCCCCE
LASASDLAVIVCGGVTTTAEHLQTLRATLR
EECCCCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVIAALASSGVACVSSVPTASRPMPLHIPTPYIRSPIASRRLGRTVRLKLDALQPSGSF
EEEEEHHCCCCEEECCCCCCCCCEEEECCCHHHHCCHHHHHCCCEEEEEEEEECCCCCE
KLRGIGAVCEARHAAGARRFVSSSGGNAGIAVAYCGRELGVPVRVVVPESASARARELIR
EEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCEEEEECCCCCHHHHHEEE
IEGADLVVHGASWAEANAFAQSTLDEHDAFVHPFDDRVLWQGHATMIDEMAAAGPKPDAV
ECCCEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCEEECCCHHHHHHHHHCCCCCCEE
VLAVGGGGLLCGVLEGLARNGWHDVPVVAAETEGADCYARSVAQGRPVELAAIASIATSL
EEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHH
GAKRPCDAAVEWATRHAIHPVVVSDADAVAASLRFVDEHRIVVEPACGAALAALERPVPV
CCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHEEECCEEEEECCCCHHHHHHCCCCCE
LASASDLAVIVCGGVTTTAEHLQTLRATLR
EECCCCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: @THRE01.txt*L-threonine!; H2O [C]

Specific reaction: @THRE01.txt*L-threonine! + H2O = oxobutanoate + NH3 + H2O [C]

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]