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The map label for this gene is lpdV [H]

Identifier: 116689268

GI number: 116689268

Start: 1376887

End: 1378278

Strand: Direct

Name: lpdV [H]

Synonym: Bcen2424_1246

Alternate gene names: 116689268

Gene position: 1376887-1378278 (Clockwise)

Preceding gene: 116689267

Following gene: 116689269

Centisome position: 39.52

GC content: 71.19

Gene sequence:

>1392_bases
ATGAAGAACGAACACACCACACTGCTCGTCATCGGCGGCGGCCCGGGCGGTTACGTCGCCGCGATCCGCGCCGGCCAGCT
CGGCATTCCGACCGTGCTCGTCGAACGCGACCGGCTCGGCGGCACCTGCCTGAACATCGGCTGCATTCCGTCGAAGGCGC
TGATCCACGTGGCCGACGCGTTCGAGCAGGCGTGCGGTCATGCCGGTGAAGCGGCGCTCGGCATCCGCGTGCGCACGCCG
GAGATCGACATCGCGAAAAGCGTCGCGTGGAAGGACGGCATCGTCGACCGGCTGACGCGCGGCGTCGGCGCGCTGCTCAA
GAAGAACGGCGTGCGCGTGCTGCACGGCGAAGCGCGCGTGATCGACGGCAAGACCGTCGAGGTCGTCAGCGGCGGCCACA
CGGTGCGGATCGGTTGCGAGCACCTGTTGCTCGCGACCGGGTCGGAGCCGGTCGAACTGCCGTCGATGCCGTTCGGCGGG
CACGTCGTGTCGTCCACCGACGCGCTGTCGCCCGCGACGCTGCCGAAGCGGCTGGTCGTCGTCGGAGCCGGCTATATCGG
GCTCGAACTCGGGATCGTCTATCGCAAGCTCGGCGTCGACGTCAGCGTCGTCGAGGCGGCCGAGCGCGTGCTGCCTGCGT
ACGATGCCGAACTCGTGCGGCCGGTGGCCGATTCGCTCGCGCGCCTCGGCGTGCGGCTGTGGCTCGGCCACACCGTGCTC
GGGCTGGACGAGCACGGCGCGGTGCGCGTGCGGGCGGCTGACGGCGCCGAGCAGACGCTGCCGGCCGATCGCGTGCTGGT
TGCCGTCGGCCGCAGGCCGCGCGTCGACGGCTTCGGGCTCGAGACGCTGATGCTCGATCGCAATGGCCGCGCGCTGCGGA
TCGACGACGCCTGCCGGACGTCGATGCGCAACGTGTGGGCGATCGGCGACGTCGCGGGCGAGCCGATGCTCGCGCATCGC
GCGATGGCGCAAGGCGAGATGGTGGCCGAGCTGATCGCCGGCCGGCGTCGCCAGTTCACGCCCGCGGCGATTCCAGCCGT
GTGCTTCACCGATCCCGAAATCGTGACGGCCGGCTGGTCGCCGGACGATGCACATGCGGCCGGCGTCGATTGCCTGAGCG
CGTCGTTTCCGTTCGCCGCGAACGGGCGGGCGATGACGTTGCAGGCGACCGACGGATTCGTGCGCGTCGTCGCGCGGCGC
GACAATCATCTGATCGTCGGCTGGCAGGCGGTCGGGCGCGGGGTGTCGGAGCTGGCCGCGGCGTTCTCGCAGTCGCTGGA
GATGGGCGCGCGGCTGGAAGACATCGGCGGCACGATCCACGCGCATCCGACGCTCGGCGAAGCGCTTCAGGAGGCGGCGT
TGCGGGCGCTCGGACACGCGCTGCATGTCTGA

Upstream 100 bases:

>100_bases
TTCGACCATCGCGTCGTCGACGGCGCGGACGCGGCCGAATTCATCCAGGCCGTGCGCGCTGTGCTCGAACGCCCGGCACT
GCTGTTCGTGGAGTGAGCCG

Downstream 100 bases:

>100_bases
CGTGCGGGCTTGACGGAGCGCTGCCGGTGCGTCGCGCACGCATCGGCGGCGCTTGCGGCGTCTGACGTATTTCATGTTGT
TCATTCGGTATCCGCTCGTG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERDRLGGTCLNIGCIPSKALIHVADAFEQACGHAGEAALGIRVRTP
EIDIAKSVAWKDGIVDRLTRGVGALLKKNGVRVLHGEARVIDGKTVEVVSGGHTVRIGCEHLLLATGSEPVELPSMPFGG
HVVSSTDALSPATLPKRLVVVGAGYIGLELGIVYRKLGVDVSVVEAAERVLPAYDAELVRPVADSLARLGVRLWLGHTVL
GLDEHGAVRVRAADGAEQTLPADRVLVAVGRRPRVDGFGLETLMLDRNGRALRIDDACRTSMRNVWAIGDVAGEPMLAHR
AMAQGEMVAELIAGRRRQFTPAAIPAVCFTDPEIVTAGWSPDDAHAAGVDCLSASFPFAANGRAMTLQATDGFVRVVARR
DNHLIVGWQAVGRGVSELAAAFSQSLEMGARLEDIGGTIHAHPTLGEALQEAALRALGHALHV

Sequences:

>Translated_463_residues
MKNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERDRLGGTCLNIGCIPSKALIHVADAFEQACGHAGEAALGIRVRTP
EIDIAKSVAWKDGIVDRLTRGVGALLKKNGVRVLHGEARVIDGKTVEVVSGGHTVRIGCEHLLLATGSEPVELPSMPFGG
HVVSSTDALSPATLPKRLVVVGAGYIGLELGIVYRKLGVDVSVVEAAERVLPAYDAELVRPVADSLARLGVRLWLGHTVL
GLDEHGAVRVRAADGAEQTLPADRVLVAVGRRPRVDGFGLETLMLDRNGRALRIDDACRTSMRNVWAIGDVAGEPMLAHR
AMAQGEMVAELIAGRRRQFTPAAIPAVCFTDPEIVTAGWSPDDAHAAGVDCLSASFPFAANGRAMTLQATDGFVRVVARR
DNHLIVGWQAVGRGVSELAAAFSQSLEMGARLEDIGGTIHAHPTLGEALQEAALRALGHALHV
>Mature_463_residues
MKNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERDRLGGTCLNIGCIPSKALIHVADAFEQACGHAGEAALGIRVRTP
EIDIAKSVAWKDGIVDRLTRGVGALLKKNGVRVLHGEARVIDGKTVEVVSGGHTVRIGCEHLLLATGSEPVELPSMPFGG
HVVSSTDALSPATLPKRLVVVGAGYIGLELGIVYRKLGVDVSVVEAAERVLPAYDAELVRPVADSLARLGVRLWLGHTVL
GLDEHGAVRVRAADGAEQTLPADRVLVAVGRRPRVDGFGLETLMLDRNGRALRIDDACRTSMRNVWAIGDVAGEPMLAHR
AMAQGEMVAELIAGRRRQFTPAAIPAVCFTDPEIVTAGWSPDDAHAAGVDCLSASFPFAANGRAMTLQATDGFVRVVARR
DNHLIVGWQAVGRGVSELAAAFSQSLEMGARLEDIGGTIHAHPTLGEALQEAALRALGHALHV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=37.8260869565217, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=28.5714285714286, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI22035672, Length=434, Percent_Identity=27.6497695852535, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI148277065, Length=455, Percent_Identity=23.7362637362637, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI33519430, Length=455, Percent_Identity=23.7362637362637, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519428, Length=455, Percent_Identity=23.7362637362637, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519426, Length=455, Percent_Identity=23.7362637362637, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI148277071, Length=455, Percent_Identity=23.7362637362637, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=23.448275862069, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI291045268, Length=427, Percent_Identity=22.9508196721311, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786307, Length=454, Percent_Identity=37.4449339207048, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=28.8329519450801, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=27.6231263383298, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=25.4424778761062, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=39.3013100436681, Blast_Score=287, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI71983429, Length=437, Percent_Identity=26.5446224256293, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=437, Percent_Identity=26.5446224256293, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17557007, Length=470, Percent_Identity=24.0425531914894, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=37.2916666666667, Blast_Score=273, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6325166, Length=466, Percent_Identity=28.755364806867, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=25.531914893617, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=39.2156862745098, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=26.7223382045929, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24640549, Length=476, Percent_Identity=26.2605042016807, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24640551, Length=476, Percent_Identity=26.2605042016807, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24640553, Length=476, Percent_Identity=26.2605042016807, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48652; Mature: 48652

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERDRLGGTCLNIGCIPSKALIHVADA
CCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHH
FEQACGHAGEAALGIRVRTPEIDIAKSVAWKDGIVDRLTRGVGALLKKNGVRVLHGEARV
HHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCEEE
IDGKTVEVVSGGHTVRIGCEHLLLATGSEPVELPSMPFGGHVVSSTDALSPATLPKRLVV
ECCCEEEEEECCCEEEEECEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEE
VGAGYIGLELGIVYRKLGVDVSVVEAAERVLPAYDAELVRPVADSLARLGVRLWLGHTVL
EECCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCEEE
GLDEHGAVRVRAADGAEQTLPADRVLVAVGRRPRVDGFGLETLMLDRNGRALRIDDACRT
EECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEECHHHHH
SMRNVWAIGDVAGEPMLAHRAMAQGEMVAELIAGRRRQFTPAAIPAVCFTDPEIVTAGWS
HHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEECCC
PDDAHAAGVDCLSASFPFAANGRAMTLQATDGFVRVVARRDNHLIVGWQAVGRGVSELAA
CCCCCCCCCHHHCCCCCCCCCCCEEEEEECCCEEEEEEECCCEEEEEHHHHCCCHHHHHH
AFSQSLEMGARLEDIGGTIHAHPTLGEALQEAALRALGHALHV
HHHHHHHHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERDRLGGTCLNIGCIPSKALIHVADA
CCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHH
FEQACGHAGEAALGIRVRTPEIDIAKSVAWKDGIVDRLTRGVGALLKKNGVRVLHGEARV
HHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCEEE
IDGKTVEVVSGGHTVRIGCEHLLLATGSEPVELPSMPFGGHVVSSTDALSPATLPKRLVV
ECCCEEEEEECCCEEEEECEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEE
VGAGYIGLELGIVYRKLGVDVSVVEAAERVLPAYDAELVRPVADSLARLGVRLWLGHTVL
EECCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCEEE
GLDEHGAVRVRAADGAEQTLPADRVLVAVGRRPRVDGFGLETLMLDRNGRALRIDDACRT
EECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEECHHHHH
SMRNVWAIGDVAGEPMLAHRAMAQGEMVAELIAGRRRQFTPAAIPAVCFTDPEIVTAGWS
HHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEECCC
PDDAHAAGVDCLSASFPFAANGRAMTLQATDGFVRVVARRDNHLIVGWQAVGRGVSELAA
CCCCCCCCCHHHCCCCCCCCCCCEEEEEECCCEEEEEEECCCEEEEEHHHHCCCHHHHHH
AFSQSLEMGARLEDIGGTIHAHPTLGEALQEAALRALGHALHV
HHHHHHHHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]