Click here to switch to the map view.

The map label for this gene is bkdB [H]

Identifier: 116689267

GI number: 116689267

Start: 1375572

End: 1376882

Strand: Direct

Name: bkdB [H]

Synonym: Bcen2424_1245

Alternate gene names: 116689267

Gene position: 1375572-1376882 (Clockwise)

Preceding gene: 116689266

Following gene: 116689268

Centisome position: 39.48

GC content: 70.02

Gene sequence:

>1311_bases
ATGGGGATTCATGTCATCAAGATGCCGGATATCGGCGAAGGGATCGCCGAGGTCGAGCTGGTGGCCTGGCATGTGGAAGT
CGGGCAGACGATCAAGGAAGACCAGCCGCTCGCCGACGTGATGACCGACAAGGCGGCGGTCGAGATTCCGTCGCCGGTGA
CGGGCAAGGTGATCGAGCTGGGCGGCCGGATCGGCGAGATGATGGCGGTGGGCAGCGAGCTGATTCGGCTGGAAGTCGAA
GGCGACGGCAACCTGAAGGCCGCGGCACCGGTGCGGGAAACGAAGGTAGCAACCGCACCGGTCGAGGCGCCGGCGCCGTC
GAAGCCGGCGGCCGACACATCGGCCGAGCCGCCTGTGCAGCCCGCCGCGCCTCGGGCACCGGCGAAGCCGCGTCGCGAGG
AACCGGCCACGCCGCCGCGCGCCGCGCTCGCGCCGGGCGAACGGCCGCTCGCGTCGCCGGCCGTGCGCCAGCGCGCGTGG
GACATGGGCATCGAGCTGCGCTACGTGCGCGGCACCGGTGAAGCGGGGCGCATCCTGCATGCCGATCTCGACGCGTATGC
GCGTACCGGCGGCGCGGCGCACGGGTCGCAACCGCGCGGCTACGACGAACGCCACGACGAAACCGAAGTGCCGGTGATCG
GCCTGCGGCGCGCGATCGCGCGCAAGATGCAGGAGGCGAAGCGCCGCATCCCGCACTTCAGCTACGTCGAGGAAATCGAC
GTGACCGAGCTCGAATCGTTGCGCACGGAACTGAACCGCCGCCACGGCGACACGCGCGGCAAGCTCACGCCGCTGCCGCT
GCTGATCCGCGCGATGGTGATCGCGCTGCGCGACTTCCCGCAGATCAATGCGCGGTTCGACGACGAAGCGGGCGTCGTCA
CGCGCTACGGCGCGGTGCACATGGGCGTCGCGACGCAGACGGACGGCGGCCTCACGGTGCCCGTGCTGCGTCATGCGGAA
GCGCGCGACGTGTGGTCCATCTCGGCGGAAATCGCACGGCTTGCCGATGCGGTGCGGGCGAACCGCGCGCAGCGCGACGA
GTTGAGCGGCTCGACGATCACGATCTCGAGCCTCGGCGCGCTGGGCGGCATCGTGTCGACGCCGGTGATCAATCATCCGG
AGGTCGGCATCGTCGGCGTGAACCGGATCGTCGAGCGGCCGATGATCCGCGACGGCGCGGTCGTCGCGCGCAAGATGATG
AACCTGTCGTCGTCGTTCGACCATCGCGTCGTCGACGGCGCGGACGCGGCCGAATTCATCCAGGCCGTGCGCGCTGTGCT
CGAACGCCCGGCACTGCTGTTCGTGGAGTGA

Upstream 100 bases:

>100_bases
ACGGGCTGGGACACCCCGTATCCGCATGCGCAGGAATGGGCGTACTTTCCGGGGCCGACCCGGGTCGGTGAAGCGTTGCG
ACGCGTGATGGAGGCGTGAG

Downstream 100 bases:

>100_bases
GCCGATGAAGAACGAACACACCACACTGCTCGTCATCGGCGGCGGCCCGGGCGGTTACGTCGCCGCGATCCGCGCCGGCC
AGCTCGGCATTCCGACCGTG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MGIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVTGKVIELGGRIGEMMAVGSELIRLEVE
GDGNLKAAAPVRETKVATAPVEAPAPSKPAADTSAEPPVQPAAPRAPAKPRREEPATPPRAALAPGERPLASPAVRQRAW
DMGIELRYVRGTGEAGRILHADLDAYARTGGAAHGSQPRGYDERHDETEVPVIGLRRAIARKMQEAKRRIPHFSYVEEID
VTELESLRTELNRRHGDTRGKLTPLPLLIRAMVIALRDFPQINARFDDEAGVVTRYGAVHMGVATQTDGGLTVPVLRHAE
ARDVWSISAEIARLADAVRANRAQRDELSGSTITISSLGALGGIVSTPVINHPEVGIVGVNRIVERPMIRDGAVVARKMM
NLSSSFDHRVVDGADAAEFIQAVRAVLERPALLFVE

Sequences:

>Translated_436_residues
MGIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVTGKVIELGGRIGEMMAVGSELIRLEVE
GDGNLKAAAPVRETKVATAPVEAPAPSKPAADTSAEPPVQPAAPRAPAKPRREEPATPPRAALAPGERPLASPAVRQRAW
DMGIELRYVRGTGEAGRILHADLDAYARTGGAAHGSQPRGYDERHDETEVPVIGLRRAIARKMQEAKRRIPHFSYVEEID
VTELESLRTELNRRHGDTRGKLTPLPLLIRAMVIALRDFPQINARFDDEAGVVTRYGAVHMGVATQTDGGLTVPVLRHAE
ARDVWSISAEIARLADAVRANRAQRDELSGSTITISSLGALGGIVSTPVINHPEVGIVGVNRIVERPMIRDGAVVARKMM
NLSSSFDHRVVDGADAAEFIQAVRAVLERPALLFVE
>Mature_435_residues
GIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVTGKVIELGGRIGEMMAVGSELIRLEVEG
DGNLKAAAPVRETKVATAPVEAPAPSKPAADTSAEPPVQPAAPRAPAKPRREEPATPPRAALAPGERPLASPAVRQRAWD
MGIELRYVRGTGEAGRILHADLDAYARTGGAAHGSQPRGYDERHDETEVPVIGLRRAIARKMQEAKRRIPHFSYVEEIDV
TELESLRTELNRRHGDTRGKLTPLPLLIRAMVIALRDFPQINARFDDEAGVVTRYGAVHMGVATQTDGGLTVPVLRHAEA
RDVWSISAEIARLADAVRANRAQRDELSGSTITISSLGALGGIVSTPVINHPEVGIVGVNRIVERPMIRDGAVVARKMMN
LSSSFDHRVVDGADAAEFIQAVRAVLERPALLFVE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=457, Percent_Identity=30.8533916849015, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=28.0459770114943, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=31.7180616740088, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI203098816, Length=233, Percent_Identity=27.8969957081545, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI203098753, Length=233, Percent_Identity=27.8969957081545, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI260898739, Length=159, Percent_Identity=28.9308176100629, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1786305, Length=296, Percent_Identity=31.4189189189189, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1786946, Length=441, Percent_Identity=26.984126984127, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=447, Percent_Identity=32.8859060402685, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI25146366, Length=441, Percent_Identity=27.437641723356, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=428, Percent_Identity=28.2710280373832, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=25.4019292604502, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=438, Percent_Identity=27.8538812785388, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=25.8695652173913, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI18859875, Length=456, Percent_Identity=29.6052631578947, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24645909, Length=231, Percent_Identity=31.1688311688312, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24582497, Length=226, Percent_Identity=30.5309734513274, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20129315, Length=226, Percent_Identity=30.5309734513274, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 46948; Mature: 46817

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVTGKVIEL
CCEEEEECCCCCCCHHHEEEEEEEEHHCCHHHCCCCHHHHHCCCCEEECCCCCCCHHHHH
GGRIGEMMAVGSELIRLEVEGDGNLKAAAPVRETKVATAPVEAPAPSKPAADTSAEPPVQ
HHHHHHHHHHCCEEEEEEECCCCCEEECCCCHHCEEEECCCCCCCCCCCCCCCCCCCCCC
PAAPRAPAKPRREEPATPPRAALAPGERPLASPAVRQRAWDMGIELRYVRGTGEAGRILH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEE
ADLDAYARTGGAAHGSQPRGYDERHDETEVPVIGLRRAIARKMQEAKRRIPHFSYVEEID
ECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
VTELESLRTELNRRHGDTRGKLTPLPLLIRAMVIALRDFPQINARFDDEAGVVTRYGAVH
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEE
MGVATQTDGGLTVPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGSTITISSLGA
ECEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHH
LGGIVSTPVINHPEVGIVGVNRIVERPMIRDGAVVARKMMNLSSSFDHRVVDGADAAEFI
HHHHHHCCCCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
QAVRAVLERPALLFVE
HHHHHHHCCCCEEEEC
>Mature Secondary Structure 
GIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVTGKVIEL
CEEEEECCCCCCCHHHEEEEEEEEHHCCHHHCCCCHHHHHCCCCEEECCCCCCCHHHHH
GGRIGEMMAVGSELIRLEVEGDGNLKAAAPVRETKVATAPVEAPAPSKPAADTSAEPPVQ
HHHHHHHHHHCCEEEEEEECCCCCEEECCCCHHCEEEECCCCCCCCCCCCCCCCCCCCCC
PAAPRAPAKPRREEPATPPRAALAPGERPLASPAVRQRAWDMGIELRYVRGTGEAGRILH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEE
ADLDAYARTGGAAHGSQPRGYDERHDETEVPVIGLRRAIARKMQEAKRRIPHFSYVEEID
ECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
VTELESLRTELNRRHGDTRGKLTPLPLLIRAMVIALRDFPQINARFDDEAGVVTRYGAVH
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEE
MGVATQTDGGLTVPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGSTITISSLGA
ECEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHH
LGGIVSTPVINHPEVGIVGVNRIVERPMIRDGAVVARKMMNLSSSFDHRVVDGADAAEFI
HHHHHHCCCCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
QAVRAVLERPALLFVE
HHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]