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The map label for this gene is lutR [H]

Identifier: 116689186

GI number: 116689186

Start: 1276232

End: 1276981

Strand: Direct

Name: lutR [H]

Synonym: Bcen2424_1164

Alternate gene names: 116689186

Gene position: 1276232-1276981 (Clockwise)

Preceding gene: 116689184

Following gene: 116689190

Centisome position: 36.63

GC content: 70.93

Gene sequence:

>750_bases
ATGTCCGTGCCTACGCTTCCCGCAGCGCCGCGCCGTCGCGCACGCAGCCTCGCACAAGATGTCGTCGACGCGCTGACCGC
GCAGATCGAAAACGGCACGCTGCGGCCCGGCGACAAGCTGCCGACCGAAACCGAAGTCATGGCGGCGCAGGGCGTGAGCC
GCACGGTCGTGCGCGAGGCGATCTCGCGGATGCAGGCGAGCGGCCTCGTCGAGACGCGCCACGGCATCGGCAGCTTCGTG
CTGGAGCCGTCGCGCCGTCAGACGCTCGGCATCGATCCCGCGACGATCACGACGCTGCGCGACGTGCTCGCGGTGCTGGA
GCTGCGTATCAGCCTCGAAAGCGAGTGCGCGAGCCTGGCCGCGCAGCGCGCGAACGATGCCGACCTCGCCGCGCTGCGGC
GCGCGCTCGACGCGATCGCGACGGGCGCGGGTGGCGGGCGTGACACCGCGCAGCTCGACTTCCAGTTCCATCTGCAGATC
GCGCAGTCCACCGGCAACCGCTATTTCGTCGACATCATGACGCAGCTGGGCACGTCGATCATTCCGCGTACGCGGGTGAA
TTCGGCGCGCTTTGCCGGCGACGACCTCGAGCGCTACGTCGGCCGGCTGAACCACGAGCACGAGGACATCTACGAGGCGA
TCGCGCGCCACGACCCGGAAGCGGCGCGCGCCGCGATGCGCACGCACCTCACCAACAGCCGCGAGCGCCTGCGGCGCGCG
CACGAGGCAGCCGAGGCCGAAGCGGGCTGA

Upstream 100 bases:

>100_bases
CGGATGACTTGTGACGCAGTATAATGAATCATCGGCTTTCGCTCAAACCCCGCGTAAACCCTCGGCTCACATTACGATCA
TTCAAAAAGGAACCGGCCAC

Downstream 100 bases:

>100_bases
CCGTTGGGTCACGGGGCTCGCGAGGTCGCGGGTCCGCTGGCATTGTTTTAACATCAGTCGTCATACGAGATCGGCGCAAT
CGTCTCGATCTCCGCGGCCA

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREAISRMQASGLVETRHGIGSFV
LEPSRRQTLGIDPATITTLRDVLAVLELRISLESECASLAAQRANDADLAALRRALDAIATGAGGGRDTAQLDFQFHLQI
AQSTGNRYFVDIMTQLGTSIIPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
HEAAEAEAG

Sequences:

>Translated_249_residues
MSVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREAISRMQASGLVETRHGIGSFV
LEPSRRQTLGIDPATITTLRDVLAVLELRISLESECASLAAQRANDADLAALRRALDAIATGAGGGRDTAQLDFQFHLQI
AQSTGNRYFVDIMTQLGTSIIPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
HEAAEAEAG
>Mature_248_residues
SVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREAISRMQASGLVETRHGIGSFVL
EPSRRQTLGIDPATITTLRDVLAVLELRISLESECASLAAQRANDADLAALRRALDAIATGAGGGRDTAQLDFQFHLQIA
QSTGNRYFVDIMTQLGTSIIPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRAH
EAAEAEAG

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082208, Length=226, Percent_Identity=33.1858407079646, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790780, Length=214, Percent_Identity=33.6448598130841, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790032, Length=236, Percent_Identity=27.5423728813559, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789353, Length=232, Percent_Identity=26.7241379310345, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI48994955, Length=224, Percent_Identity=28.125, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786303, Length=226, Percent_Identity=32.3008849557522, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1789621, Length=224, Percent_Identity=27.6785714285714, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27124; Mature: 26993

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHH
ISRMQASGLVETRHGIGSFVLEPSRRQTLGIDPATITTLRDVLAVLELRISLESECASLA
HHHHHHHCCHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRANDADLAALRRALDAIATGAGGGRDTAQLDFQFHLQIAQSTGNRYFVDIMTQLGTSI
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHEEEEEHHCCCCCHHHHHHHHHCHHH
IPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
CCCCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
HEAAEAEAG
HHHHHCCCC
>Mature Secondary Structure 
SVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHH
ISRMQASGLVETRHGIGSFVLEPSRRQTLGIDPATITTLRDVLAVLELRISLESECASLA
HHHHHHHCCHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRANDADLAALRRALDAIATGAGGGRDTAQLDFQFHLQIAQSTGNRYFVDIMTQLGTSI
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHEEEEEHHCCCCCHHHHHHHHHCHHH
IPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
CCCCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
HEAAEAEAG
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]