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The map label for this gene is yhjG [H]

Identifier: 116689052

GI number: 116689052

Start: 1139726

End: 1141372

Strand: Direct

Name: yhjG [H]

Synonym: Bcen2424_1029

Alternate gene names: 116689052

Gene position: 1139726-1141372 (Clockwise)

Preceding gene: 116689050

Following gene: 116689057

Centisome position: 32.71

GC content: 70.37

Gene sequence:

>1647_bases
ATGGCTGACACACTGCCTGATATCCCGCCCGTGCTGATCGTCGGCGCGGGGCCCACCGGTCTCGCCGCGGCGATGAGCCT
CGCACGGGCTCGCGTGCCGGTGCGCATCATCGATCGTCTCGTCGCGCCCGCGCCGTTTTCGCGTGCGATCGGCATCCAGG
CGCGCACGCTCGAACTGCTGGAGCAGCATCGCGCGGTCGAGCCGTTTCTCGCGCTCGGTCATCGCGCGCATGCGGCCGAG
CTGCACGCCGACGGCCGCGTGATCGCACGGCTCGATTTCGATCCGCTGCGAACGCGCTATCCGTATCTGATGTTTCTCGA
CCAGAGCGTTACCGAGCGGCTGCTCGCCGAGCACCTGGCCGGCATGGGCGTGAGCGTCGAACGCGGGACGGCGCTGACCG
GCTGCGATGCGGGCGGCACGTCGCTCGACGTGACGCTGCGCCGCGCCGACGGCCGCGACGAATCGTTCAGCCCGTCGTAC
CTGATTGCCGCCGACGGCGCGCACAGCACGGTGAGGCATCTGCTCGGCCTGGGCTTTGCCGGGCATGCGTTCGAACAGAC
TTTCCTGCTGGCCGATTTCGCGGCGATACCCGACTGGCCGGACGAAGAGATCCACCTGTTCACGACACCCGCCGGCATCG
CGGGCCTGTTTCCGATGGGAGGCGGCCGTTACCGGCTCGTCGCGGACCGGCCTCCGCGCAGCGACGCGTCGCCCGACGCG
CCGGCGCCGTCGCTCGACGAATGCGACGCGATCATCCGCGCGCGCGTCGGCGCATCGATCTCGCCGAGCGATCTCGCGTG
GTCGTCCTATTTTCACCTGCACAGCCGGATGGTCGACCGGCTCCGTCACGGCCGCGTGTTCTTCGCGGGCGACGCGGCGC
ACGTTCACAGCCCGGCCGGTGCGCAGGGCATGAACACCGGCATCCAGGAAGCGTTCAACCTGGGCTGGAAGCTCGCCCGC
GTCCTCGGCGCAGGCGCGCCCGAGCGATTGCTCGACACGTATCACGCGGAGCGCCACCCGATCGAGCGGGACGTATTGCG
GCAGACCGGTTTCGTCACGCAGATCGTCGAAGCCGAGCGCGGCGCGATGAAGCTGCTGCGTGATCACGTCGTGCCGCTGC
TGGCGTCGTTCGGGCCGATGCGCGACGCGGTCAGGCGCACGGTCAGCGAGCTCGGCGTGCAATACCGGAAGAGTCCGCTC
ACGCTCGAGCGCGTGCTCGATGGCGGCCCACGGGCAGGCGAGCGGGCGCCCGACGCACTCGTACACGTGCTCGACGGGCC
GCTCGGCCGCGCGCCCGGCACGGCGCGGCTATACGATATTCACGATCCGGCCTGCTTCACGCTGTTGCTGCTGGAAGAGC
CGGTGGATACCGACGCCGGCGAGGTGCCGCCGATCCCGGCCGATGCGCAGGCGCTCGTGCAGGGGCTCGAACGGATCATG
CCGGAAGCGGTGCGCGTTTGGCGCGTCGCCGATGCCGAAGGCGATGGTACGGCCGGGCTCGCGCAGGAATACGGCCGCTC
GCGCCCGTCGTTCTATCTGCTGCGGCCCGACGGCTACGTTGCCGCGCGCGGGCGCACGGCGACCGACGCGAACGCGCTGC
TGCGCCACTGCGAAACCTGGTTCGCGGGCGTGTCGGTATCCGCGTAG

Upstream 100 bases:

>100_bases
TCCCACACCGCGCGGCGGGGCCGCGTATCGGCCGGTCGGCATATGGCCCCGTGACGCGGAGCGGCGTAGACTTGCAGAGT
CGCGTGTCATCGAGGTTCTC

Downstream 100 bases:

>100_bases
CCGCGCGCGCGGTCAGGCGGGCGCCGCGGCAGGGACGAGCGACGCGCGATGCGCGGCGATCGCATCGCGCACGATCGGCG
TCGCGCGTTGCCCGGCTTCG

Product: monooxygenase, FAD-binding

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MADTLPDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLVAPAPFSRAIGIQARTLELLEQHRAVEPFLALGHRAHAAE
LHADGRVIARLDFDPLRTRYPYLMFLDQSVTERLLAEHLAGMGVSVERGTALTGCDAGGTSLDVTLRRADGRDESFSPSY
LIAADGAHSTVRHLLGLGFAGHAFEQTFLLADFAAIPDWPDEEIHLFTTPAGIAGLFPMGGGRYRLVADRPPRSDASPDA
PAPSLDECDAIIRARVGASISPSDLAWSSYFHLHSRMVDRLRHGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLAR
VLGAGAPERLLDTYHAERHPIERDVLRQTGFVTQIVEAERGAMKLLRDHVVPLLASFGPMRDAVRRTVSELGVQYRKSPL
TLERVLDGGPRAGERAPDALVHVLDGPLGRAPGTARLYDIHDPACFTLLLLEEPVDTDAGEVPPIPADAQALVQGLERIM
PEAVRVWRVADAEGDGTAGLAQEYGRSRPSFYLLRPDGYVAARGRTATDANALLRHCETWFAGVSVSA

Sequences:

>Translated_548_residues
MADTLPDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLVAPAPFSRAIGIQARTLELLEQHRAVEPFLALGHRAHAAE
LHADGRVIARLDFDPLRTRYPYLMFLDQSVTERLLAEHLAGMGVSVERGTALTGCDAGGTSLDVTLRRADGRDESFSPSY
LIAADGAHSTVRHLLGLGFAGHAFEQTFLLADFAAIPDWPDEEIHLFTTPAGIAGLFPMGGGRYRLVADRPPRSDASPDA
PAPSLDECDAIIRARVGASISPSDLAWSSYFHLHSRMVDRLRHGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLAR
VLGAGAPERLLDTYHAERHPIERDVLRQTGFVTQIVEAERGAMKLLRDHVVPLLASFGPMRDAVRRTVSELGVQYRKSPL
TLERVLDGGPRAGERAPDALVHVLDGPLGRAPGTARLYDIHDPACFTLLLLEEPVDTDAGEVPPIPADAQALVQGLERIM
PEAVRVWRVADAEGDGTAGLAQEYGRSRPSFYLLRPDGYVAARGRTATDANALLRHCETWFAGVSVSA
>Mature_547_residues
ADTLPDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLVAPAPFSRAIGIQARTLELLEQHRAVEPFLALGHRAHAAEL
HADGRVIARLDFDPLRTRYPYLMFLDQSVTERLLAEHLAGMGVSVERGTALTGCDAGGTSLDVTLRRADGRDESFSPSYL
IAADGAHSTVRHLLGLGFAGHAFEQTFLLADFAAIPDWPDEEIHLFTTPAGIAGLFPMGGGRYRLVADRPPRSDASPDAP
APSLDECDAIIRARVGASISPSDLAWSSYFHLHSRMVDRLRHGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLARV
LGAGAPERLLDTYHAERHPIERDVLRQTGFVTQIVEAERGAMKLLRDHVVPLLASFGPMRDAVRRTVSELGVQYRKSPLT
LERVLDGGPRAGERAPDALVHVLDGPLGRAPGTARLYDIHDPACFTLLLLEEPVDTDAGEVPPIPADAQALVQGLERIMP
EAVRVWRVADAEGDGTAGLAQEYGRSRPSFYLLRPDGYVAARGRTATDANALLRHCETWFAGVSVSA

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=404, Percent_Identity=27.2277227722772, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 59096; Mature: 58965

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTLPDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLVAPAPFSRAIGIQARTLELL
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHCCHHHHHHHH
EQHRAVEPFLALGHRAHAAELHADGRVIARLDFDPLRTRYPYLMFLDQSVTERLLAEHLA
HHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCHHHHCCCEEEEECHHHHHHHHHHHHH
GMGVSVERGTALTGCDAGGTSLDVTLRRADGRDESFSPSYLIAADGAHSTVRHLLGLGFA
CCCCEECCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCCC
GHAFEQTFLLADFAAIPDWPDEEIHLFTTPAGIAGLFPMGGGRYRLVADRPPRSDASPDA
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCCCCCCEEEEEECCCCCCCCCCCC
PAPSLDECDAIIRARVGASISPSDLAWSSYFHLHSRMVDRLRHGRVFFAGDAAHVHSPAG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCC
AQGMNTGIQEAFNLGWKLARVLGAGAPERLLDTYHAERHPIERDVLRQTGFVTQIVEAER
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHH
GAMKLLRDHVVPLLASFGPMRDAVRRTVSELGVQYRKSPLTLERVLDGGPRAGERAPDAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHH
VHVLDGPLGRAPGTARLYDIHDPACFTLLLLEEPVDTDAGEVPPIPADAQALVQGLERIM
HHHHCCCCCCCCCCCEEEECCCCHHEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
PEAVRVWRVADAEGDGTAGLAQEYGRSRPSFYLLRPDGYVAARGRTATDANALLRHCETW
HHHHHHHEEECCCCCCCCHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHH
FAGVSVSA
HCCCCCCC
>Mature Secondary Structure 
ADTLPDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLVAPAPFSRAIGIQARTLELL
CCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHCCHHHHHHHH
EQHRAVEPFLALGHRAHAAELHADGRVIARLDFDPLRTRYPYLMFLDQSVTERLLAEHLA
HHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCHHHHCCCEEEEECHHHHHHHHHHHHH
GMGVSVERGTALTGCDAGGTSLDVTLRRADGRDESFSPSYLIAADGAHSTVRHLLGLGFA
CCCCEECCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCCC
GHAFEQTFLLADFAAIPDWPDEEIHLFTTPAGIAGLFPMGGGRYRLVADRPPRSDASPDA
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCCCCCCEEEEEECCCCCCCCCCCC
PAPSLDECDAIIRARVGASISPSDLAWSSYFHLHSRMVDRLRHGRVFFAGDAAHVHSPAG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCC
AQGMNTGIQEAFNLGWKLARVLGAGAPERLLDTYHAERHPIERDVLRQTGFVTQIVEAER
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHH
GAMKLLRDHVVPLLASFGPMRDAVRRTVSELGVQYRKSPLTLERVLDGGPRAGERAPDAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHH
VHVLDGPLGRAPGTARLYDIHDPACFTLLLLEEPVDTDAGEVPPIPADAQALVQGLERIM
HHHHCCCCCCCCCCCEEEECCCCHHEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
PEAVRVWRVADAEGDGTAGLAQEYGRSRPSFYLLRPDGYVAARGRTATDANALLRHCETW
HHHHHHHEEECCCCCCCCHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHH
FAGVSVSA
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]