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The map label for this gene is citH [H]

Identifier: 116689050

GI number: 116689050

Start: 1136384

End: 1137679

Strand: Direct

Name: citH [H]

Synonym: Bcen2424_1027

Alternate gene names: 116689050

Gene position: 1136384-1137679 (Clockwise)

Preceding gene: 116689048

Following gene: 116689052

Centisome position: 32.62

GC content: 65.74

Gene sequence:

>1296_bases
ATGTCCACAGCGTCCCCTGCGATCGCGCAGGAATCGAAAGTCCGCACCGTCTTCCGGGTCGTCAGCGGCAACTTCCTGGA
AATGTACGACTTCATGGTCTACGGGTATTACGCGTCGGCCATCGCGAAAACGTACTTTCCGAGCGGCAACGCGTTCGCGT
CGCTGATGCTGTCGCTGTCGGTATTCGGCGCGGGTTTCCTGATGCGCCCGGTCGGTGCGATCGTGCTCGGCGCGTACATC
GATCATCACGGCCGCCGCAAGGGCCTGATCCTGACGCTCGGGCTGATGGCGATCGGCACGCTCACCGTGGCCGCGATCCC
GGGCTATGCGACGATCGGCGTGCTCGCGCCGGTGCTCGTGCTGCTCGGCCGGTTGTTGCAGGGCTTCTCGGCGGGCGTCG
AGCTCGGCGGCGTGTCGGTCTACCTGTCGGAGATCGCGACGAAGGGCCACAAGGGGTTCTATACGTCGTGGCAGTCGGGC
AGCCAGCAGGTGGCCGTCGTGTTCGCCGCGTTCGTCGGTGTCGTGCTGAACCGCGCGCTGCCGGTCGAGGAAATGACCGC
GTGGGGCTGGCGCATTCCGTTCCTGATCGGCTGCCTGATCGTGCCGTTCCTGTTCCTGATCCGCCGGTCGCTGAAGGAGA
CCGACGAGTTTCTTGCGAAGCGTCACCGGCCGACGATGGGCGAGATCATGCGCTCGATGCTCGACAACTGGGGTGTCGTG
GTGGCCGGCATGGGGATGGTGATCATGACGACAGTGTCGTTCTACATGATTACCGCGTACACGCCGACCTTCGGCAAGGA
AGTGCTGCACCTGTCGTCGCTCGACGCGCTCGTCGTGACCGTTTGCGTCGGGATCTCGAATCTCGTGTGGTTGCCGCTGT
CCGGCGCGCTGTCCGACCGCATCGGTCGCCGCCCGGTGCTGATCGCCTTCACCGTGCTGACGCTGGTGTCGGCTTATCCG
GCCGTGCTGTGGCTCGTCGGCGATCCGTCGTTCCTGCGGCTGCTCGCGGTCGAGCTGTGGCTGTCGTTCCTGTACGCGTC
GTACAACGGCGCGATGGTCGTCGCGCTGACCGAAGTGATGCCGGCCGACGTGCGTACGGCCGGCTTCTCGCTCGCGTACA
GCCTGGCCACGACGATCGGCGGCTTCACGCCGGCGATCTCGACGCTGCTGATCCACCAGACGGGCAACAAGGCGGCGCCG
GGGCTGTGGCTGAGCGTGGCCGCGATCTGCGGCCTGATCGCGACGCTCGTGCTGTATCGCACGGCCGATGCGCGCAACCA
GTACAAGTCAGCCTGA

Upstream 100 bases:

>100_bases
GCAGCGATGCCCGGGCGCGCGGGGCTCACCCTGCGCGTCGCGCATCGTCGGATTCATGCGGCGGCGCGGTATTCGCGCCA
CCCGAGCTGAGCGAGAAAAT

Downstream 100 bases:

>100_bases
CGGCGGCCGGTATATCGCGCGGTGTCGGCCGCGCATCACATCGAAAAGGGAGCGCGATGCGCTCCCTTTTTCGCTTGCGG
TCATGCGTTGCGCAGCATGT

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSTASPAIAQESKVRTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYI
DHHGRRKGLILTLGLMAIGTLTVAAIPGYATIGVLAPVLVLLGRLLQGFSAGVELGGVSVYLSEIATKGHKGFYTSWQSG
SQQVAVVFAAFVGVVLNRALPVEEMTAWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRHRPTMGEIMRSMLDNWGVV
VAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSSLDALVVTVCVGISNLVWLPLSGALSDRIGRRPVLIAFTVLTLVSAYP
AVLWLVGDPSFLRLLAVELWLSFLYASYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHQTGNKAAP
GLWLSVAAICGLIATLVLYRTADARNQYKSA

Sequences:

>Translated_431_residues
MSTASPAIAQESKVRTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYI
DHHGRRKGLILTLGLMAIGTLTVAAIPGYATIGVLAPVLVLLGRLLQGFSAGVELGGVSVYLSEIATKGHKGFYTSWQSG
SQQVAVVFAAFVGVVLNRALPVEEMTAWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRHRPTMGEIMRSMLDNWGVV
VAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSSLDALVVTVCVGISNLVWLPLSGALSDRIGRRPVLIAFTVLTLVSAYP
AVLWLVGDPSFLRLLAVELWLSFLYASYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHQTGNKAAP
GLWLSVAAICGLIATLVLYRTADARNQYKSA
>Mature_430_residues
STASPAIAQESKVRTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYID
HHGRRKGLILTLGLMAIGTLTVAAIPGYATIGVLAPVLVLLGRLLQGFSAGVELGGVSVYLSEIATKGHKGFYTSWQSGS
QQVAVVFAAFVGVVLNRALPVEEMTAWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRHRPTMGEIMRSMLDNWGVVV
AGMGMVIMTTVSFYMITAYTPTFGKEVLHLSSLDALVVTVCVGISNLVWLPLSGALSDRIGRRPVLIAFTVLTLVSAYPA
VLWLVGDPSFLRLLAVELWLSFLYASYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHQTGNKAAPG
LWLSVAAICGLIATLVLYRTADARNQYKSA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=430, Percent_Identity=30.4651162790698, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788942, Length=431, Percent_Identity=32.9466357308585, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788292, Length=405, Percent_Identity=28.641975308642, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789941, Length=425, Percent_Identity=30.5882352941176, Blast_Score=142, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46341; Mature: 46210

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCC
>Mature Secondary Structure 
STASPAIAQESKVRTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLS
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH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HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]