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The map label for this gene is ribE [H]

Identifier: 116688982

GI number: 116688982

Start: 1065466

End: 1066095

Strand: Reverse

Name: ribE [H]

Synonym: Bcen2424_0959

Alternate gene names: 116688982

Gene position: 1066095-1065466 (Counterclockwise)

Preceding gene: 116688983

Following gene: 116688981

Centisome position: 30.6

GC content: 65.08

Gene sequence:

>630_bases
ATGTTTACCGGAATTGTCGCGGCCGTCGGCCGCATCGAAACGATCGCCCCGCTCGGCACGTCGGCCGATGCGGGCGTGCG
CCTGACCGTGCAGGCCGGCGGGCTCGATCTCGCCGATGTCGCGCTGGGCGACAGCATCGCGATCCAGGGCGCATGCATGA
CGGTGATCGAGAAGACCGACACCACGTTCGACGTCGACGTGTCGCGCGAAAGCCTGAACCGGACGGTCGGCCTCGCGCAG
CCCGGCGAAGTGAATCTCGAGAAGGCGCTGCGCGCGCATGACCGCCTCGGCGGGCATATCGTGTCGGGCCATGTGGACGG
CCTCGGTACCGTGTCGCGCTTCGCACCGGTCGGCGAGTCGCACGAACTGCGGATCATCGCGCCGCGCGAACTCGGCCGCT
ATCTCGCGTACAAGGGATCAATCACGGTGAACGGTGTCAGCCTGACCGTCAATACGATCGACGACCGCGCGGACGGCTGC
GAATTCTCGATCAACCTGATTCCGCATACGGTCGAAGTCACGACGCTGCGTCACGTGAAGGCCGGCGACAAGGTCAATCT
CGAAGTCGACATGATCGCGCGATATGTGGAACGGATGCTGTCGGCGTCGCAGGGCGTGCATCAGGACTGA

Upstream 100 bases:

>100_bases
CCCGGCTGTCGTTCCACGGTATCGAGCGGGTCGGCGACGACCTGCGCATCCTCGCGCGCGTCGCACCGACCGACACGTCT
CACTGAACGGAATCGCCACG

Downstream 100 bases:

>100_bases
ACAAGCAGATCCTCTGTATATGGAATGACGGCTGGCGATTCGGCATTGTTCGGTCGCCACGGCCCCCTCGCTCCTCCACT
CACAGGCCGCCGTGCCGTGC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MFTGIVAAVGRIETIAPLGTSADAGVRLTVQAGGLDLADVALGDSIAIQGACMTVIEKTDTTFDVDVSRESLNRTVGLAQ
PGEVNLEKALRAHDRLGGHIVSGHVDGLGTVSRFAPVGESHELRIIAPRELGRYLAYKGSITVNGVSLTVNTIDDRADGC
EFSINLIPHTVEVTTLRHVKAGDKVNLEVDMIARYVERMLSASQGVHQD

Sequences:

>Translated_209_residues
MFTGIVAAVGRIETIAPLGTSADAGVRLTVQAGGLDLADVALGDSIAIQGACMTVIEKTDTTFDVDVSRESLNRTVGLAQ
PGEVNLEKALRAHDRLGGHIVSGHVDGLGTVSRFAPVGESHELRIIAPRELGRYLAYKGSITVNGVSLTVNTIDDRADGC
EFSINLIPHTVEVTTLRHVKAGDKVNLEVDMIARYVERMLSASQGVHQD
>Mature_209_residues
MFTGIVAAVGRIETIAPLGTSADAGVRLTVQAGGLDLADVALGDSIAIQGACMTVIEKTDTTFDVDVSRESLNRTVGLAQ
PGEVNLEKALRAHDRLGGHIVSGHVDGLGTVSRFAPVGESHELRIIAPRELGRYLAYKGSITVNGVSLTVNTIDDRADGC
EFSINLIPHTVEVTTLRHVKAGDKVNLEVDMIARYVERMLSASQGVHQD

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=211, Percent_Identity=31.7535545023697, Blast_Score=89, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319733, Length=208, Percent_Identity=36.0576923076923, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22154; Mature: 22154

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVAAVGRIETIAPLGTSADAGVRLTVQAGGLDLADVALGDSIAIQGACMTVIEKTD
CCCHHHHHHCCHHEECCCCCCCCCCEEEEEEECCCEEEHHHCCCCEEECHHEEEEEECCC
TTFDVDVSRESLNRTVGLAQPGEVNLEKALRAHDRLGGHIVSGHVDGLGTVSRFAPVGES
CEEEEECCHHHHHHEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCC
HELRIIAPRELGRYLAYKGSITVNGVSLTVNTIDDRADGCEFSINLIPHTVEVTTLRHVK
CCEEEECHHHHCCCEEEECCEEEEEEEEEEEECCCCCCCCEEEEEECCCEEEEEEEEEEC
AGDKVNLEVDMIARYVERMLSASQGVHQD
CCCEEEEEHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFTGIVAAVGRIETIAPLGTSADAGVRLTVQAGGLDLADVALGDSIAIQGACMTVIEKTD
CCCHHHHHHCCHHEECCCCCCCCCCEEEEEEECCCEEEHHHCCCCEEECHHEEEEEECCC
TTFDVDVSRESLNRTVGLAQPGEVNLEKALRAHDRLGGHIVSGHVDGLGTVSRFAPVGES
CEEEEECCHHHHHHEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCC
HELRIIAPRELGRYLAYKGSITVNGVSLTVNTIDDRADGCEFSINLIPHTVEVTTLRHVK
CCEEEECHHHHCCCEEEECCEEEEEEEEEEEECCCCCCCCEEEEEECCCEEEEEEEEEEC
AGDKVNLEVDMIARYVERMLSASQGVHQD
CCCEEEEEHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144477 [H]