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The map label for this gene is ytwF [H]

Identifier: 116688934

GI number: 116688934

Start: 1021510

End: 1021833

Strand: Reverse

Name: ytwF [H]

Synonym: Bcen2424_0911

Alternate gene names: 116688934

Gene position: 1021833-1021510 (Counterclockwise)

Preceding gene: 116688935

Following gene: 116688933

Centisome position: 29.33

GC content: 68.21

Gene sequence:

>324_bases
ATGCAGATCCTGACGCCCGCGATGCTCGCGGAATGGCTCGGCGACACGGCGCGCCCCGCGCCGGTCGTGCTCGACGTGCG
CGAGCCGTGGGAAATCGCGACCGCGCAGATCGCCGGCAGCGTGGCGATTCCGATGCAGCAGATTCCCGCGCGCAGCGAAG
AGCTCGACGACGAAGCGGAAATCGTCTGCGTGTGCCATCACGGGATGCGCAGCGCGCAGGTCGCGATGTTCCTCGAATCG
CGCGGCTTCACGAAGCTGTACAACCTGCAGGGCGGCATCGACGCGTGGTCGCGCGACGTCGATCCGTCCGTGCCGCGCTA
CTGA

Upstream 100 bases:

>100_bases
ACAAGCAGTACCGCGTCGCCGACGTGTTCGAAACGTACATCGACCACTTGGTCAACGCGATCGAGCCGTCGCGCTTCAAG
TTCTGAGCGGCGGAGCCTCC

Downstream 100 bases:

>100_bases
TCGCCCCTTCCGGCGGCGCATCATGCGTCGCCGCGCCGGCCGGCACCCAGCCGCCGGCCGCACCGATCCGCCGAACGGGC
GCGCACCGCGCGCCCGTTTT

Product: rhodanese domain-containing protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 107; Mature: 107

Protein sequence:

>107_residues
MQILTPAMLAEWLGDTARPAPVVLDVREPWEIATAQIAGSVAIPMQQIPARSEELDDEAEIVCVCHHGMRSAQVAMFLES
RGFTKLYNLQGGIDAWSRDVDPSVPRY

Sequences:

>Translated_107_residues
MQILTPAMLAEWLGDTARPAPVVLDVREPWEIATAQIAGSVAIPMQQIPARSEELDDEAEIVCVCHHGMRSAQVAMFLES
RGFTKLYNLQGGIDAWSRDVDPSVPRY
>Mature_107_residues
MQILTPAMLAEWLGDTARPAPVVLDVREPWEIATAQIAGSVAIPMQQIPARSEELDDEAEIVCVCHHGMRSAQVAMFLES
RGFTKLYNLQGGIDAWSRDVDPSVPRY

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11837; Mature: 11837

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILTPAMLAEWLGDTARPAPVVLDVREPWEIATAQIAGSVAIPMQQIPARSEELDDEAE
CCCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCCCC
IVCVCHHGMRSAQVAMFLESRGFTKLYNLQGGIDAWSRDVDPSVPRY
EEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQILTPAMLAEWLGDTARPAPVVLDVREPWEIATAQIAGSVAIPMQQIPARSEELDDEAE
CCCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCCCC
IVCVCHHGMRSAQVAMFLESRGFTKLYNLQGGIDAWSRDVDPSVPRY
EEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]