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The map label for this gene is pcm [H]

Identifier: 116688935

GI number: 116688935

Start: 1021848

End: 1022504

Strand: Reverse

Name: pcm [H]

Synonym: Bcen2424_0912

Alternate gene names: 116688935

Gene position: 1022504-1021848 (Counterclockwise)

Preceding gene: 116688936

Following gene: 116688934

Centisome position: 29.35

GC content: 63.77

Gene sequence:

>657_bases
ATGAATATCGAAAACGCGCGTTTCAACATGATCGAACAGCAGATCCGTCCGTGGGACGTGCTGGATCTGGATGTCCTGGG
CCTGCTGTCGATCGTCAAGCGTGAAAACTACGTGCCAGCCGAATACCGCGATCTCGCGTTCGCCGATCTCGAACTGCCGC
TGCCCGGCGGCGCCAGCAAGATGCTGTTCCCGCGCGTCGAAGCGCGCGTGCTGCAGGAACTGACGGTCAAGAAGCACGAA
AACGTGCTCGTGATCGGCGCGGGCTCGGGCTACCTGGCCGCACTGTTCGCGCATCGCGCGCAACACGTGACGGCCGTCGA
GATCGATCCGACGATCGCGAAATTCGCGGAAGACAACCTGCGCAACGACGGCGTGACCAACGCCGAAGTCGTGCTCGGCG
ACGGTTCGCGCGGCTGGGCCGCGAAGGCGCCGTACGACGTGATCTGCGTCGCGGGCGGCCTGCCGGTCGTGCCGCAGGAA
ATGCTCGAGCAGCTGAAGATCGGCGGCCGCCTGTCGGCGTTCGTCGGCGGCCGTCCGGTGATGAAGGCGCAGATCATCAC
CCGCATCGACGACAAGCAGTACCGCGTCGCCGACGTGTTCGAAACGTACATCGACCACTTGGTCAACGCGATCGAGCCGT
CGCGCTTCAAGTTCTGA

Upstream 100 bases:

>100_bases
CTGTTGCAGGCCGCCTGAAGGCTTCCCGGCCGTGCCGTTTGGTACAATCACGAAGTTTCTCTATCCCCGCTGATGGCCGA
CCAGGCTGCATGACCACACC

Downstream 100 bases:

>100_bases
GCGGCGGAGCCTCCATGCAGATCCTGACGCCCGCGATGCTCGCGGAATGGCTCGGCGACACGGCGCGCCCCGCGCCGGTC
GTGCTCGACGTGCGCGAGCC

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MNIENARFNMIEQQIRPWDVLDLDVLGLLSIVKRENYVPAEYRDLAFADLELPLPGGASKMLFPRVEARVLQELTVKKHE
NVLVIGAGSGYLAALFAHRAQHVTAVEIDPTIAKFAEDNLRNDGVTNAEVVLGDGSRGWAAKAPYDVICVAGGLPVVPQE
MLEQLKIGGRLSAFVGGRPVMKAQIITRIDDKQYRVADVFETYIDHLVNAIEPSRFKF

Sequences:

>Translated_218_residues
MNIENARFNMIEQQIRPWDVLDLDVLGLLSIVKRENYVPAEYRDLAFADLELPLPGGASKMLFPRVEARVLQELTVKKHE
NVLVIGAGSGYLAALFAHRAQHVTAVEIDPTIAKFAEDNLRNDGVTNAEVVLGDGSRGWAAKAPYDVICVAGGLPVVPQE
MLEQLKIGGRLSAFVGGRPVMKAQIITRIDDKQYRVADVFETYIDHLVNAIEPSRFKF
>Mature_218_residues
MNIENARFNMIEQQIRPWDVLDLDVLGLLSIVKRENYVPAEYRDLAFADLELPLPGGASKMLFPRVEARVLQELTVKKHE
NVLVIGAGSGYLAALFAHRAQHVTAVEIDPTIAKFAEDNLRNDGVTNAEVVLGDGSRGWAAKAPYDVICVAGGLPVVPQE
MLEQLKIGGRLSAFVGGRPVMKAQIITRIDDKQYRVADVFETYIDHLVNAIEPSRFKF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=140, Percent_Identity=37.1428571428571, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1789100, Length=170, Percent_Identity=34.7058823529412, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24169; Mature: 24169

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIENARFNMIEQQIRPWDVLDLDVLGLLSIVKRENYVPAEYRDLAFADLELPLPGGASK
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCEEEEEEECCCCCCCC
MLFPRVEARVLQELTVKKHENVLVIGAGSGYLAALFAHRAQHVTAVEIDPTIAKFAEDNL
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCEEEEEECHHHHHHHHHHC
RNDGVTNAEVVLGDGSRGWAAKAPYDVICVAGGLPVVPQEMLEQLKIGGRLSAFVGGRPV
CCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEHHCCCCH
MKAQIITRIDDKQYRVADVFETYIDHLVNAIEPSRFKF
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNIENARFNMIEQQIRPWDVLDLDVLGLLSIVKRENYVPAEYRDLAFADLELPLPGGASK
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCEEEEEEECCCCCCCC
MLFPRVEARVLQELTVKKHENVLVIGAGSGYLAALFAHRAQHVTAVEIDPTIAKFAEDNL
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCEEEEEECHHHHHHHHHHC
RNDGVTNAEVVLGDGSRGWAAKAPYDVICVAGGLPVVPQEMLEQLKIGGRLSAFVGGRPV
CCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEHHCCCCH
MKAQIITRIDDKQYRVADVFETYIDHLVNAIEPSRFKF
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA