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The map label for this gene is rfbD [H]

Identifier: 116688897

GI number: 116688897

Start: 977418

End: 978317

Strand: Direct

Name: rfbD [H]

Synonym: Bcen2424_0874

Alternate gene names: 116688897

Gene position: 977418-978317 (Clockwise)

Preceding gene: 116688896

Following gene: 116688898

Centisome position: 28.06

GC content: 66.11

Gene sequence:

>900_bases
ATGCGTGAGGTGGGTGTGACTCCTGAGCCGACGATTCTCGTGACGGGTGTGAATGGCCAGGTCGGGTTCGAACTGCTGCG
CTCGCTGCAAGGACTCGGCCGCGTGGTCGCATGCGACCGCTCGATGCTCGACCTGTCCGACCTCGATCGGGTGCGCAGCG
TCGTGCGCGAGCTGAAGCCGTCGATCATCGTGAACCCGGCCGCGTACACGGCCGTCGACAAGGCCGAGACGGACGTCGAC
GCTGCGCGCCGGCTGAATGTCGAAGTCCCGCGTGCTTTCGCAGAGGAGGCGGCGCGCCTCGGTGCAGCGCTGGTTCATTA
CTCAACCGACTATGTGTTCGACGGCACCAAAGAGGGTGCCTATGTCGAAACGGACGCGACCAACCCGCAGAACGTCTACG
GGCTGACCAAGCTGGAGGGCGAGCAGGCCATCGCGGCGACAGGTTGCGCACACTTGATCCTGCGGACGAGCTGGGTGTAC
GGCCGCCGCGGCAAGAACTTCCTGCTGACGATGCTGAAGCTCGGGAGCGAGCGTCCTGAGCTGCGTGTCGTCGCGGATCA
GGTTGGCGCGCCGACATGGTCGAAGACGATCGCCACCGCGACCGCGCACATTGTCGCGCAGGCGTTGGCGGCCGATGACG
CGGACTGGTGGGCGCAGCGTTCCGGCGTCTATCACTTCACGTCCGCGGGCGCCACGTCGTGGCACGGGTTTGCCGAGGCA
ATCTTCGCCCAGGCGATGGGCGAGCGTGCGCCGAGGGTGTTGCCGATTCCGGCGAGCGACTACCCGGTTCCCGCCAAGCG
CCCGTCCAATTCGCGACTGTCGCACGACAAGCTGACCGAAGCATTCGGGTTGCGGTTGCCGGACTGGGCCGATGCGCTGA
CGCTGTGCCTGAGTGAGTAA

Upstream 100 bases:

>100_bases
TGGAACGACCCGGAGATCGGGATCGAGTGGCCGATCGATTTCGAGCCGCTGCTGGCGGCGAAGGATGCGGCCGGCAAGCG
TTTGAGCGAAGCCGAAGTTT

Downstream 100 bases:

>100_bases
CTTGAATCAGAATTCCGCGGTTCAGGAGCAGACCGGTGTCGTGATCGTGTTCTACCGACCGGATAGCGGTTGCGTAGCCC
GTGCGAACCGGCTCGCCAAA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MREVGVTPEPTILVTGVNGQVGFELLRSLQGLGRVVACDRSMLDLSDLDRVRSVVRELKPSIIVNPAAYTAVDKAETDVD
AARRLNVEVPRAFAEEAARLGAALVHYSTDYVFDGTKEGAYVETDATNPQNVYGLTKLEGEQAIAATGCAHLILRTSWVY
GRRGKNFLLTMLKLGSERPELRVVADQVGAPTWSKTIATATAHIVAQALAADDADWWAQRSGVYHFTSAGATSWHGFAEA
IFAQAMGERAPRVLPIPASDYPVPAKRPSNSRLSHDKLTEAFGLRLPDWADALTLCLSE

Sequences:

>Translated_299_residues
MREVGVTPEPTILVTGVNGQVGFELLRSLQGLGRVVACDRSMLDLSDLDRVRSVVRELKPSIIVNPAAYTAVDKAETDVD
AARRLNVEVPRAFAEEAARLGAALVHYSTDYVFDGTKEGAYVETDATNPQNVYGLTKLEGEQAIAATGCAHLILRTSWVY
GRRGKNFLLTMLKLGSERPELRVVADQVGAPTWSKTIATATAHIVAQALAADDADWWAQRSGVYHFTSAGATSWHGFAEA
IFAQAMGERAPRVLPIPASDYPVPAKRPSNSRLSHDKLTEAFGLRLPDWADALTLCLSE
>Mature_299_residues
MREVGVTPEPTILVTGVNGQVGFELLRSLQGLGRVVACDRSMLDLSDLDRVRSVVRELKPSIIVNPAAYTAVDKAETDVD
AARRLNVEVPRAFAEEAARLGAALVHYSTDYVFDGTKEGAYVETDATNPQNVYGLTKLEGEQAIAATGCAHLILRTSWVY
GRRGKNFLLTMLKLGSERPELRVVADQVGAPTWSKTIATATAHIVAQALAADDADWWAQRSGVYHFTSAGATSWHGFAEA
IFAQAMGERAPRVLPIPASDYPVPAKRPSNSRLSHDKLTEAFGLRLPDWADALTLCLSE

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=299, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI11034825, Length=299, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1788352, Length=297, Percent_Identity=44.1077441077441, Blast_Score=240, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32418; Mature: 32418

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREVGVTPEPTILVTGVNGQVGFELLRSLQGLGRVVACDRSMLDLSDLDRVRSVVRELKP
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCC
SIIVNPAAYTAVDKAETDVDAARRLNVEVPRAFAEEAARLGAALVHYSTDYVFDGTKEGA
CEEECCHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCEEECCCCCCC
YVETDATNPQNVYGLTKLEGEQAIAATGCAHLILRTSWVYGRRGKNFLLTMLKLGSERPE
EEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
LRVVADQVGAPTWSKTIATATAHIVAQALAADDADWWAQRSGVYHFTSAGATSWHGFAEA
EEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCHHHHHHHH
IFAQAMGERAPRVLPIPASDYPVPAKRPSNSRLSHDKLTEAFGLRLPDWADALTLCLSE
HHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MREVGVTPEPTILVTGVNGQVGFELLRSLQGLGRVVACDRSMLDLSDLDRVRSVVRELKP
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCC
SIIVNPAAYTAVDKAETDVDAARRLNVEVPRAFAEEAARLGAALVHYSTDYVFDGTKEGA
CEEECCHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCEEECCCCCCC
YVETDATNPQNVYGLTKLEGEQAIAATGCAHLILRTSWVYGRRGKNFLLTMLKLGSERPE
EEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
LRVVADQVGAPTWSKTIATATAHIVAQALAADDADWWAQRSGVYHFTSAGATSWHGFAEA
EEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCHHHHHHHH
IFAQAMGERAPRVLPIPASDYPVPAKRPSNSRLSHDKLTEAFGLRLPDWADALTLCLSE
HHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]