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The map label for this gene is rfbB [H]

Identifier: 116688894

GI number: 116688894

Start: 974923

End: 975984

Strand: Direct

Name: rfbB [H]

Synonym: Bcen2424_0871

Alternate gene names: 116688894

Gene position: 974923-975984 (Clockwise)

Preceding gene: 116688892

Following gene: 116688895

Centisome position: 27.98

GC content: 64.41

Gene sequence:

>1062_bases
ATGATCCTGGTTACGGGCGGCGCGGGTTTTATCGGTGCCAATTTTGTACTCGACTGGCTGCGTGCATCCGACGAAGCCGT
GCTCAACGTCGACAAGCTGACGTACGCAGGGAACCTGCGCACGCTGCAGTCGCTGGACGGAAACCCGAAGCACGTGTTCG
TGCGCGTCGACATCTGCGACCGTGACGCGCTGGACGCCCTCCTGGCCGAGCACAAGCCCCGCGCGGTGCTGCACTTCGCC
GCGGAAAGCCATGTCGATCGCTCGATCCACGGTCCCGCGGACTTCGTGCAGACCAACGTCGTCGGCACGTTCACGCTGCT
CGAGGCCACCCGCCAGTACTGGAACGGTCTGAACGACGCCGACAAGGCCGCGTTCCGTTTCCTGCACGTGTCGACCGACG
AGGTGTTCGGTTCGCTGTCGGCCACCGACCCGCAATTCTCCGAGACGACACCGTACGCGCCGAACAGCCCGTATTCCGCG
ACGAAGGCGGGCTCCGACCACCTGGTGCGCGCGTACCATCATACGTATGGCCTGCCGGTGCTGACGACGAACTGTTCGAA
CAACTACGGCCCGTACCAGTTCCCGGAAAAGCTGATCCCGCTGATGATCGCGAACGCGCTCGCGGGCAAGCCGCTGCCGG
TGTATGGCGACGGCCAGAACGTGCGCGACTGGCTGTACGTCGGCGACCACTGCAGCGCGATCCGCGAAGTGCTCGCGCGT
GGCGTACCGGGCGAGACGTACAACGTCGGAGGCTGGAACGAGAAGAAGAACCTCGAAGTCGTGCATACGCTGTGCGACCT
GCTCGACAAGGCGCGCCCGAAGGCAGCGGGTTCGTACCGTGACCAGATCACCTATGTGACCGACCGGCCCGGCCACGATC
GCCGCTACGCGATCGATGCGCGCAAGCTCGAACGCGAACTCGGCTGGAAGCCTGCGGAAACGTTCGAGACCGGCCTGGCG
AAGACGGTCGACTGGTATCTCGACAACCAGGTGTGGGCCGATGAGGTCGCATCGGGCGAGTACCGCAAGTGGGTCGAGAC
GAACTACGCGAAACGCACGTGA

Upstream 100 bases:

>100_bases
AATTCCCGGTTGGACGTGGCAATCACAAGCTACTAGCATTTGCGAATCTGAGGTGGCAGGCGCCTGATGGCGTCTGCCGC
ACACTAATTGAGGGAGCCTC

Downstream 100 bases:

>100_bases
GGAGAGAAGCGATGGCACGTAAAGGCATCATTCTCGCCGGCGGTTCCGGCACCCGGCTGTATCCGATCACGCACGTCGTC
TCGAAGCAGCTTCTGCCGGT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILVTGGAGFIGANFVLDWLRASDEAVLNVDKLTYAGNLRTLQSLDGNPKHVFVRVDICDRDALDALLAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEATRQYWNGLNDADKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLEVVHTLCDLLDKARPKAAGSYRDQITYVTDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVDWYLDNQVWADEVASGEYRKWVETNYAKRT

Sequences:

>Translated_353_residues
MILVTGGAGFIGANFVLDWLRASDEAVLNVDKLTYAGNLRTLQSLDGNPKHVFVRVDICDRDALDALLAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEATRQYWNGLNDADKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLEVVHTLCDLLDKARPKAAGSYRDQITYVTDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVDWYLDNQVWADEVASGEYRKWVETNYAKRT
>Mature_353_residues
MILVTGGAGFIGANFVLDWLRASDEAVLNVDKLTYAGNLRTLQSLDGNPKHVFVRVDICDRDALDALLAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEATRQYWNGLNDADKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLEVVHTLCDLLDKARPKAAGSYRDQITYVTDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVDWYLDNQVWADEVASGEYRKWVETNYAKRT

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=34.3465045592705, Blast_Score=218, Evalue=9e-57,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=25.6797583081571, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI56237023, Length=345, Percent_Identity=27.8260869565217, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI56118217, Length=345, Percent_Identity=27.8260869565217, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI189083684, Length=345, Percent_Identity=27.8260869565217, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4504031, Length=336, Percent_Identity=25.5952380952381, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=60.4456824512535, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=61.9186046511628, Blast_Score=446, Evalue=1e-127,
Organism=Escherichia coli, GI1786974, Length=353, Percent_Identity=26.628895184136, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788366, Length=301, Percent_Identity=27.906976744186, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=36.3095238095238, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=31.9148936170213, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=23.8670694864048, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=364, Percent_Identity=27.7472527472527, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=366, Percent_Identity=27.5956284153005, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=334, Percent_Identity=23.9520958083832, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=336, Percent_Identity=24.4047619047619, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=336, Percent_Identity=24.4047619047619, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=336, Percent_Identity=24.4047619047619, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=336, Percent_Identity=24.4047619047619, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=336, Percent_Identity=24.4047619047619, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=363, Percent_Identity=26.4462809917355, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19923002, Length=364, Percent_Identity=30.2197802197802, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356223, Length=339, Percent_Identity=25.0737463126844, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=26.2686567164179, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39376; Mature: 39376

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGANFVLDWLRASDEAVLNVDKLTYAGNLRTLQSLDGNPKHVFVRVDICD
CEEEECCCCHHHHHHHHHHHHCCCCCEEEEHHEEECCCCHHHHHCCCCCCEEEEEEEECC
RDALDALLAEHKPRAVLHFAAESHVDRSIHGPADFVQTNVVGTFTLLEATRQYWNGLNDA
CHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPV
HHHHEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GVPGETYNVGGWNEKKNLEVVHTLCDLLDKARPKAAGSYRDQITYVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEHHH
RKLERELGWKPAETFETGLAKTVDWYLDNQVWADEVASGEYRKWVETNYAKRT
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MILVTGGAGFIGANFVLDWLRASDEAVLNVDKLTYAGNLRTLQSLDGNPKHVFVRVDICD
CEEEECCCCHHHHHHHHHHHHCCCCCEEEEHHEEECCCCHHHHHCCCCCCEEEEEEEECC
RDALDALLAEHKPRAVLHFAAESHVDRSIHGPADFVQTNVVGTFTLLEATRQYWNGLNDA
CHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPV
HHHHEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GVPGETYNVGGWNEKKNLEVVHTLCDLLDKARPKAAGSYRDQITYVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEHHH
RKLERELGWKPAETFETGLAKTVDWYLDNQVWADEVASGEYRKWVETNYAKRT
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]