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The map label for this gene is grpE

Identifier: 116688773

GI number: 116688773

Start: 840407

End: 840952

Strand: Direct

Name: grpE

Synonym: Bcen2424_0750

Alternate gene names: 116688773

Gene position: 840407-840952 (Clockwise)

Preceding gene: 116688770

Following gene: 116688774

Centisome position: 24.12

GC content: 67.22

Gene sequence:

>546_bases
ATGGAAAACACGCAAGAGAACCCGACTACCCCATCGGCCGAAGACATCGGCAGCGAGAAGCAGGCGGCGCAAGGCGCCGC
ACCCGCTGCCGAAGCTGCCGACGCGGCGCTCGCGGAGGCTCAAGCCAAGGTCGCCGAGCTCCAGGAAAGCTTCCTGCGGG
CGAAGGCCGAGACCGAGAACGTGCGCCGCCGCGCGCAGGACGACGTGTCGAAGGCGCACAAGTTCGCGATCGAGAGCTTC
GCGGAACACCTGCTGCCCGTGCTGGACAGCCTGGAAGCGGCCGTCAACGATACCTCCGGCGACATCGCGAAGGTGCGCGA
AGGCGTCGAGCTGACGCTGCGCCAGCTGACGAGCGCGCTCGAGAAGGGCCGCGTCGTCGCGATCAACCCGATCGGCGAGA
AGTTCGATCCGCACCAGCACCAGGCCATTTCGATGGTGCCGGCCGAGCAGGAACCGAACACCGTCGTCTCGGTGCTGCAA
AAGGGCTATACGATCGCCGACCGCGTGCTGCGCCCGGCGCTCGTCACCGTCGCCCAGCCGAAGTAA

Upstream 100 bases:

>100_bases
GCAGTTTTCGCCCTTATTTGCACCAGTAATGTGCGGTGCCGCCGGGTATACGTCCGACGGTTGCCGATTTGGCTTCAACC
TCTAATCGACTTTCAGCGAC

Downstream 100 bases:

>100_bases
GGCGGCCGACATGGTGCCCGCCGGCGTCGATCCGGCCGCGTTCGACACGTTCGACATGCAGGCGCTCGACGCCGGCACGT
TCGACGCGACCATCGACGGC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MENTQENPTTPSAEDIGSEKQAAQGAAPAAEAADAALAEAQAKVAELQESFLRAKAETENVRRRAQDDVSKAHKFAIESF
AEHLLPVLDSLEAAVNDTSGDIAKVREGVELTLRQLTSALEKGRVVAINPIGEKFDPHQHQAISMVPAEQEPNTVVSVLQ
KGYTIADRVLRPALVTVAQPK

Sequences:

>Translated_181_residues
MENTQENPTTPSAEDIGSEKQAAQGAAPAAEAADAALAEAQAKVAELQESFLRAKAETENVRRRAQDDVSKAHKFAIESF
AEHLLPVLDSLEAAVNDTSGDIAKVREGVELTLRQLTSALEKGRVVAINPIGEKFDPHQHQAISMVPAEQEPNTVVSVLQ
KGYTIADRVLRPALVTVAQPK
>Mature_181_residues
MENTQENPTTPSAEDIGSEKQAAQGAAPAAEAADAALAEAQAKVAELQESFLRAKAETENVRRRAQDDVSKAHKFAIESF
AEHLLPVLDSLEAAVNDTSGDIAKVREGVELTLRQLTSALEKGRVVAINPIGEKFDPHQHQAISMVPAEQEPNTVVSVLQ
KGYTIADRVLRPALVTVAQPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=188, Percent_Identity=36.1702127659575, Blast_Score=101, Evalue=3e-22,
Organism=Homo sapiens, GI40255109, Length=179, Percent_Identity=35.7541899441341, Blast_Score=88, Evalue=4e-18,
Organism=Escherichia coli, GI1788967, Length=152, Percent_Identity=43.421052631579, Blast_Score=119, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17552458, Length=160, Percent_Identity=35.625, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=154, Percent_Identity=32.4675324675325, Blast_Score=92, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653432, Length=167, Percent_Identity=35.9281437125749, Blast_Score=100, Evalue=6e-22,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_BURCA (Q1BYX5)

Other databases:

- EMBL:   CP000378
- RefSeq:   YP_620153.1
- ProteinModelPortal:   Q1BYX5
- SMR:   Q1BYX5
- STRING:   Q1BYX5
- GeneID:   4092214
- GenomeReviews:   CP000378_GR
- KEGG:   bcn:Bcen_0266
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   EVEPNSV
- ProtClustDB:   PRK14139
- BioCyc:   BCEN331271:BCEN_0266-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 19398; Mature: 19398

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTQENPTTPSAEDIGSEKQAAQGAAPAAEAADAALAEAQAKVAELQESFLRAKAETEN
CCCCCCCCCCCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRRRAQDDVSKAHKFAIESFAEHLLPVLDSLEAAVNDTSGDIAKVREGVELTLRQLTSAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EKGRVVAINPIGEKFDPHQHQAISMVPAEQEPNTVVSVLQKGYTIADRVLRPALVTVAQP
HCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCC
K
C
>Mature Secondary Structure
MENTQENPTTPSAEDIGSEKQAAQGAAPAAEAADAALAEAQAKVAELQESFLRAKAETEN
CCCCCCCCCCCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRRRAQDDVSKAHKFAIESFAEHLLPVLDSLEAAVNDTSGDIAKVREGVELTLRQLTSAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EKGRVVAINPIGEKFDPHQHQAISMVPAEQEPNTVVSVLQKGYTIADRVLRPALVTVAQP
HCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA