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The map label for this gene is nitA [H]

Identifier: 116688727

GI number: 116688727

Start: 783926

End: 784849

Strand: Direct

Name: nitA [H]

Synonym: Bcen2424_0704

Alternate gene names: 116688727

Gene position: 783926-784849 (Clockwise)

Preceding gene: 116688725

Following gene: 116688730

Centisome position: 22.5

GC content: 71.86

Gene sequence:

>924_bases
ATGTCCACCTCAGTCATCGCCGCGCTGCAGATCGGCGCATCGCCCGCCGGCACGCGCGCCACGCTCGACACGATCCTCGG
CTACGAAACCGCGATCCGCGACAGCGGCGCGTCGCTGGTCGTGCTGCCCGAGGCCGTGCTAGGCGGCTATCCGAAAGGCG
AGATCTTCGGCACGCGGCTCGGTTACCGGCTACCCGAGGGGCGCGACGCGTATGCGCGCTACGCCGCGCAAGCGATCGAC
GTGCCGGGGCCGGAAACCGACGAGCTGGCCGCGCTGTCGCAGCGCACCGGCGCGAGCCTCGTGGTCGGCGTGATCGAGTG
CGGCGGCAGCACGCTGTACTGCACGGCGCTGTTCTTCGATCCGCGCGACGGGCTCGTCGCGAAGCACCGCAAGCTGATGC
CGACCGGCACCGAGCGGCTGATCTGGGGGCAGGGCGACGGCTCGACGCTGCCGGTCGTCGACACGGCCGCAGGCCGCGCG
GGCGCCGCGATCTGCTGGGAGAACCACATGCCGCTGCTGCGCTGCGCGATGTACGCGAAAGGCGTGCAGATCTGGTGTGC
GCCGACCGTCGACGAGCGCGACGTGTGGCAGAGCTCGATGCGGCACATCGCGCACGAAGGGCGCTGCTTCGTCGTGAGCG
CGTGCCAGGTGCAGCCGTCGCCGCGCGCGCTCGGCATCGACGTGCCGGGCTGGGACCCCGAGCGGCCGCTGATCCGCGGC
GGCAGCGTGATCATCGGGCCGCTCGGCGACCTGCTGGCGGAGCCGCTGATCGGCGAGGCCGGGCTCGTCACGGCGCGCAT
CGACACCGACGAGCTCGTGAAGGCGCGCTACGACTTCGACGTCGTCGGCCACTATGCGCGTGCGGACGTGTTTTCGCTGC
ACGTCGACGAGCGGCCGAAGCGGCCGGTGGTGTTCGGCGGGTGA

Upstream 100 bases:

>100_bases
TCCATATTCATGTCAGTTATCGTTGTATCGATTTGATCGATGAAATGATATCGACAAAGATGGCGTCATCCCACTCACCG
CATTCACCGGAGACGCCTTC

Downstream 100 bases:

>100_bases
CGAAGTGGGGCCGGGCGCCACATGGCGCCCGGAAAACGAAGGGCGGCGGTGCGTGCCCGCCGCCTTGCCACGTCAGCGCG
TCAGCGCATCGGCGCTTCCG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSTSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRLGYRLPEGRDAYARYAAQAID
VPGPETDELAALSQRTGASLVVGVIECGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRA
GAAICWENHMPLLRCAMYAKGVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
GSVIIGPLGDLLAEPLIGEAGLVTARIDTDELVKARYDFDVVGHYARADVFSLHVDERPKRPVVFGG

Sequences:

>Translated_307_residues
MSTSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRLGYRLPEGRDAYARYAAQAID
VPGPETDELAALSQRTGASLVVGVIECGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRA
GAAICWENHMPLLRCAMYAKGVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
GSVIIGPLGDLLAEPLIGEAGLVTARIDTDELVKARYDFDVVGHYARADVFSLHVDERPKRPVVFGG
>Mature_306_residues
STSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRLGYRLPEGRDAYARYAAQAIDV
PGPETDELAALSQRTGASLVVGVIECGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRAG
AAICWENHMPLLRCAMYAKGVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRGG
SVIIGPLGDLLAEPLIGEAGLVTARIDTDELVKARYDFDVVGHYARADVFSLHVDERPKRPVVFGG

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=306, Percent_Identity=46.4052287581699, Blast_Score=276, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6322027, Length=199, Percent_Identity=64.8241206030151, Blast_Score=265, Evalue=9e-72,
Organism=Saccharomyces cerevisiae, GI6322026, Length=111, Percent_Identity=47.7477477477478, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 32738; Mature: 32607

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRL
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCEEEEEC
GYRLPEGRDAYARYAAQAIDVPGPETDELAALSQRTGASLVVGVIECGGSTLYCTALFFD
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEEEC
PRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRAGAAICWENHMPLLRCAMYAK
CCCCHHHHHHCCCCCCCCEEEEECCCCCEECEEECCCCCCCEEEEECCCCHHHHHHHHHC
GVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
CEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCEEEC
GSVIIGPLGDLLAEPLIGEAGLVTARIDTDELVKARYDFDVVGHYARADVFSLHVDERPK
CCEEEECCHHHHHCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHCEEEEEEECCCCC
RPVVFGG
CCEEECC
>Mature Secondary Structure 
STSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRL
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCEEEEEC
GYRLPEGRDAYARYAAQAIDVPGPETDELAALSQRTGASLVVGVIECGGSTLYCTALFFD
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEEEC
PRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRAGAAICWENHMPLLRCAMYAK
CCCCHHHHHHCCCCCCCCEEEEECCCCCEECEEECCCCCCCEEEEECCCCHHHHHHHHHC
GVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
CEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCEEEC
GSVIIGPLGDLLAEPLIGEAGLVTARIDTDELVKARYDFDVVGHYARADVFSLHVDERPK
CCEEEECCHHHHHCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHCEEEEEEECCCCC
RPVVFGG
CCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]