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The map label for this gene is murF [H]

Identifier: 116688579

GI number: 116688579

Start: 623950

End: 625356

Strand: Direct

Name: murF [H]

Synonym: Bcen2424_0555

Alternate gene names: 116688579

Gene position: 623950-625356 (Clockwise)

Preceding gene: 116688578

Following gene: 116688580

Centisome position: 17.91

GC content: 71.86

Gene sequence:

>1407_bases
ATGACGATGCTCAGCCTCGGCGAAGCCGCCCGCCTGATTCCCGGCGCCACCGTCCACGGCGACGCGGGCGTCACGTTCAA
CCGCGTGTCGACCGATAGCCGCACGGTCGGCGCGGGCGACCTGTTCGTCGCGCTGAAGGGCGAGCGTTTCGATGCGCACG
ACTTTCTCGGCGACGTTGCCGCGCGCGGCGCTGCCGCGGCGCTCGTCGCGCACGCGCCGGCGGGCATTGCGATGCCGATG
ATCGAAGGCGGCGAAACGCGTGCCGCGCTCGGCGCGCTCGCGCACGGCTGGCGGATGCGATTCCCGCTGCCGCTCGTCGC
GGTGACGGGCAGCAACGGCAAGACGACCGTCAAGGAAATGATCGCGTCGATCTTCGCGGCGGCGGTCGGCGCCGACGCAC
GGCTCGCGACGGCCGGCAACCTGAACAACGATGTCGGCCTGCCGCTGACGCTGCTGCGCCTGTCGGCCGCGCATCGTCTC
GCGGTGATCGAGATCGGCATGAACCATCCGGGCGAAACCGAAGTCCTCGCGCGCCTCACCGCGCCGACGGTCGCGCTCGT
CAACAACGCGCAGCGCGAGCACCAGGAATTCATGGCGACGGTCGACGCCGTCGCGCTCGAACATGCGGCCGTGATTCACG
CGCTGCCGCCGGACGGCGTCGCGGTGTTCCCGGCCGACGACGCGTACGCGGGCATCTGGCGCGTCGCGGCGACCGGCAAC
CGCATCCTCGATTTCGCGCTGCACGACGCCGAACGGCAGAACGACGCACAGGTCGTCGGGCGCATGCACGGCGGCGAACT
CGCGATCGACACGCCGGCGGGCGCCGTCACGGTGCGGCTGCGCGCGCTCGGCGAGCACAACGCGCGGAACGCGCTGGCCG
CGACGGCCGCGGCGCTCGGCGCCGGCGTCGCGCTGTCGGCGATCCGGCAGGGTCTCGAAGCGTTCGAACCGGTCAAGGGC
CGGCTGCAGGTGAAGCAGGCGAGCGTCGGCAGCCTCGGGGGCGCGACGGTCGTCGACGATACGTACAACGCGAATCCCGA
TTCGATGCGTGCCGCGATCGACGTGCTCGCCGCGCATCCGGCGCCGCGCGTGCTGGTAATCGGCGACATGGGCGAAGTCG
GCGACGAAGGGCCGGCCTTCCATCGCGAGATCGGCGCGTACGCGCGCGAACGCGGGATCGACGCGCTGTTCGCGCTCGGC
GACGCATCGCGCGACGCGTGCACGGCCTACGGCGACACGGCACGCCATTTCGACGACGTCGGCGCGCTCGTGTCGGCGCT
GCTCGCGGCCGGTTACGGCGCGCAGGCAACGGTGCTCGTGAAGGGCTCGCGGTACATGAAGATGGAGCGCGTGGTCGACG
CGCTGACGAACCAACCCGCGGCGGGCACCGTGCCTGCCGCACACTGA

Upstream 100 bases:

>100_bases
CCACGCAGGAAATCATGGGCAAGAAGCGTACGTTCTCCGACCAGGATCACGCGCGGCTCGCGCTCGCAGCGCGCGCGACG
CACGGCAAGGGAGGCGCCGA

Downstream 100 bases:

>100_bases
GTAGAAGGAAGGAAGCATGCTGCTGGCGCTGGCGCAATGGCTGCAAGGAGACGCAAGCTTTTTGCGCTTGTTCACGTACC
TCACGTTCCGGGCGGTGATG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTMLSLGEAARLIPGATVHGDAGVTFNRVSTDSRTVGAGDLFVALKGERFDAHDFLGDVAARGAAAALVAHAPAGIAMPM
IEGGETRAALGALAHGWRMRFPLPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNLNNDVGLPLTLLRLSAAHRL
AVIEIGMNHPGETEVLARLTAPTVALVNNAQREHQEFMATVDAVALEHAAVIHALPPDGVAVFPADDAYAGIWRVAATGN
RILDFALHDAERQNDAQVVGRMHGGELAIDTPAGAVTVRLRALGEHNARNALAATAAALGAGVALSAIRQGLEAFEPVKG
RLQVKQASVGSLGGATVVDDTYNANPDSMRAAIDVLAAHPAPRVLVIGDMGEVGDEGPAFHREIGAYARERGIDALFALG
DASRDACTAYGDTARHFDDVGALVSALLAAGYGAQATVLVKGSRYMKMERVVDALTNQPAAGTVPAAH

Sequences:

>Translated_468_residues
MTMLSLGEAARLIPGATVHGDAGVTFNRVSTDSRTVGAGDLFVALKGERFDAHDFLGDVAARGAAAALVAHAPAGIAMPM
IEGGETRAALGALAHGWRMRFPLPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNLNNDVGLPLTLLRLSAAHRL
AVIEIGMNHPGETEVLARLTAPTVALVNNAQREHQEFMATVDAVALEHAAVIHALPPDGVAVFPADDAYAGIWRVAATGN
RILDFALHDAERQNDAQVVGRMHGGELAIDTPAGAVTVRLRALGEHNARNALAATAAALGAGVALSAIRQGLEAFEPVKG
RLQVKQASVGSLGGATVVDDTYNANPDSMRAAIDVLAAHPAPRVLVIGDMGEVGDEGPAFHREIGAYARERGIDALFALG
DASRDACTAYGDTARHFDDVGALVSALLAAGYGAQATVLVKGSRYMKMERVVDALTNQPAAGTVPAAH
>Mature_467_residues
TMLSLGEAARLIPGATVHGDAGVTFNRVSTDSRTVGAGDLFVALKGERFDAHDFLGDVAARGAAAALVAHAPAGIAMPMI
EGGETRAALGALAHGWRMRFPLPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNLNNDVGLPLTLLRLSAAHRLA
VIEIGMNHPGETEVLARLTAPTVALVNNAQREHQEFMATVDAVALEHAAVIHALPPDGVAVFPADDAYAGIWRVAATGNR
ILDFALHDAERQNDAQVVGRMHGGELAIDTPAGAVTVRLRALGEHNARNALAATAAALGAGVALSAIRQGLEAFEPVKGR
LQVKQASVGSLGGATVVDDTYNANPDSMRAAIDVLAAHPAPRVLVIGDMGEVGDEGPAFHREIGAYARERGIDALFALGD
ASRDACTAYGDTARHFDDVGALVSALLAAGYGAQATVLVKGSRYMKMERVVDALTNQPAAGTVPAAH

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=436, Percent_Identity=42.6605504587156, Blast_Score=270, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48145; Mature: 48014

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00318 HMG_COA_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLSLGEAARLIPGATVHGDAGVTFNRVSTDSRTVGAGDLFVALKGERFDAHDFLGDVA
CCCCCCCCHHHHCCCCEEECCCCCEEEEECCCCCEECCCEEEEEEECCCCCHHHHHHHHH
ARGAAAALVAHAPAGIAMPMIEGGETRAALGALAHGWRMRFPLPLVAVTGSNGKTTVKEM
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCEEECCCEEEEEECCCCCHHHHHH
IASIFAAAVGADARLATAGNLNNDVGLPLTLLRLSAAHRLAVIEIGMNHPGETEVLARLT
HHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHC
APTVALVNNAQREHQEFMATVDAVALEHAAVIHALPPDGVAVFPADDAYAGIWRVAATGN
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCCEEEEEECCC
RILDFALHDAERQNDAQVVGRMHGGELAIDTPAGAVTVRLRALGEHNARNALAATAAALG
EEEEEHHHCCCCCCCHHHHHEECCCEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHH
AGVALSAIRQGLEAFEPVKGRLQVKQASVGSLGGATVVDDTYNANPDSMRAAIDVLAAHP
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC
APRVLVIGDMGEVGDEGPAFHREIGAYARERGIDALFALGDASRDACTAYGDTARHFDDV
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCHHHHHHHH
GALVSALLAAGYGAQATVLVKGSRYMKMERVVDALTNQPAAGTVPAAH
HHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TMLSLGEAARLIPGATVHGDAGVTFNRVSTDSRTVGAGDLFVALKGERFDAHDFLGDVA
CCCCCCCHHHHCCCCEEECCCCCEEEEECCCCCEECCCEEEEEEECCCCCHHHHHHHHH
ARGAAAALVAHAPAGIAMPMIEGGETRAALGALAHGWRMRFPLPLVAVTGSNGKTTVKEM
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCEEECCCEEEEEECCCCCHHHHHH
IASIFAAAVGADARLATAGNLNNDVGLPLTLLRLSAAHRLAVIEIGMNHPGETEVLARLT
HHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHC
APTVALVNNAQREHQEFMATVDAVALEHAAVIHALPPDGVAVFPADDAYAGIWRVAATGN
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCCEEEEEECCC
RILDFALHDAERQNDAQVVGRMHGGELAIDTPAGAVTVRLRALGEHNARNALAATAAALG
EEEEEHHHCCCCCCCHHHHHEECCCEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHH
AGVALSAIRQGLEAFEPVKGRLQVKQASVGSLGGATVVDDTYNANPDSMRAAIDVLAAHP
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC
APRVLVIGDMGEVGDEGPAFHREIGAYARERGIDALFALGDASRDACTAYGDTARHFDDV
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCHHHHHHHH
GALVSALLAAGYGAQATVLVKGSRYMKMERVVDALTNQPAAGTVPAAH
HHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]