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The map label for this gene is murE [H]

Identifier: 116688578

GI number: 116688578

Start: 622406

End: 623953

Strand: Direct

Name: murE [H]

Synonym: Bcen2424_0554

Alternate gene names: 116688578

Gene position: 622406-623953 (Clockwise)

Preceding gene: 116688577

Following gene: 116688579

Centisome position: 17.87

GC content: 70.74

Gene sequence:

>1548_bases
ATGAGCGCCGCCCGCAGTTCCCATCCGGCGCATCAGCAGATCGCAGCGGCGCTTGCGTGGCTGCGTCAGCATGTGGCCCC
CACGGCGCAACTGCATGCCGACACGCGCAGCCTCGAGGCTGGCGACGTGTTCGTCGCGTATGCGGTCGACGGGGCAGATA
ACCGCGCTTTCATGGCGGATGCCGTCGCACGCGGTGCGGCCGCCGTGCTGTATCAGCCGGAAGGGCTGGCCCACGCACCG
GCCGCGCCGGCCGTACCCGTCGCGCTGGCGGTGCCGGCGCTCGACCAGCTCGCCGGCGAGATCGCCAGCGGTTGGTACGG
CGACCCGAGCGACAGCTTGCTGGCGGTCGGTGTCACGGGCACGAACGGCAAGACGTCGTGCACGCAATGGATCGCCACCG
CGCTGACGGCGCTGCACCAGCCGTGCGCGGTGATCGGCACGCTCGGCACCGGGATGCCCGGTCAGCTCGTGCCGACCGGC
TTCACGACGCCCGACGCGCCGCAACTTCAGCGCAGCCTCGCGCAATTGCGCGACGCGGGTGCACAGGCGGTGGCGATGGA
GGTGTCGTCGCACGCGCTGCATCAGGGGCGCGTGAACGGGACGGCGTTCGACATCGCGGTGTTTACGAACCTCACGCAGG
ATCACCTCGACTATCACGGCACGTTCGATGCGTACGAGGCCGCGAAGGCGAAGCTGTTCGCGTGGCGCGGCTTGCGCGCG
GCGGTGATCAACCGCGACGATGCGGCTGGCCGCCGCCTGCTGGAGAAGCTGGCCGGTCGCGTGCGCACGATCGCGTACGG
GATCGGCGATGCACCGGCGCCCGACGCCGATCGCGAACTGGTCGCGCTCGACGTGCGCGCCACCGCGACGGGTACCGCAT
TCCGCCTGCGTTCGTCGTGGGGCGACGCGAACGTCGAGGTCGGCACGCTCGGCACGTTCAACGTCAGCAACCTGCTCGCG
GTGCTCGGCGCGCTGCTCGCGGCCGACGTGCCGTTTGACGCGGCACTCGCCGAGATCGAGCGGCTCGAGTCGGTCAACGG
CCGGATGCAGCGGCTCGGCGGCCGGTTGCAGAACGACGAGCCGCTCGTCGTGATCGACTACGCGCACACGCCCGACGCGC
TCGAAAAGACGCTCGACGCGCTGCGCCCGATCGCTGCGGCGCGCGGCGGCCGGCTCGTTTGCATGTTCGGCTGCGGCGGC
GATCGCGATGCGACGAAGCGTCCGCTGATGGGCGCGATTGCGGAGCGGCTCGCCGACGAAGTCGTCGTCACGAGCGACAA
CCCACGCAGCGAAGATCCGCAGCACATCATCGACCAGGTCGTCGCGGGCATGACCGCGCCTGATCGCGCACGCCGTATCG
AGGATCGCGCGAGCGCGATCCTGCAGGCGGTGCGGGGCGCCGCACGCGAGGATGTCGTCGTGCTGGCCGGCAAGGGCCAC
GAGGCCACGCAGGAAATCATGGGCAAGAAGCGTACGTTCTCCGACCAGGATCACGCGCGGCTCGCGCTCGCAGCGCGCGC
GACGCACGGCAAGGGAGGCGCCGAATGA

Upstream 100 bases:

>100_bases
AATCTGCTGCCGCGAAGGGCCCGCAAAAGCTGGCTACGAATGGCGGCGCGAAGCATATGATCGTGTCCAGCACGACGCGT
AATTCACCAGGAGTTGTTCG

Downstream 100 bases:

>100_bases
CGATGCTCAGCCTCGGCGAAGCCGCCCGCCTGATTCCCGGCGCCACCGTCCACGGCGACGCGGGCGTCACGTTCAACCGC
GTGTCGACCGATAGCCGCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSAARSSHPAHQQIAAALAWLRQHVAPTAQLHADTRSLEAGDVFVAYAVDGADNRAFMADAVARGAAAVLYQPEGLAHAP
AAPAVPVALAVPALDQLAGEIASGWYGDPSDSLLAVGVTGTNGKTSCTQWIATALTALHQPCAVIGTLGTGMPGQLVPTG
FTTPDAPQLQRSLAQLRDAGAQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRA
AVINRDDAAGRRLLEKLAGRVRTIAYGIGDAPAPDADRELVALDVRATATGTAFRLRSSWGDANVEVGTLGTFNVSNLLA
VLGALLAADVPFDAALAEIERLESVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIAAARGGRLVCMFGCGG
DRDATKRPLMGAIAERLADEVVVTSDNPRSEDPQHIIDQVVAGMTAPDRARRIEDRASAILQAVRGAAREDVVVLAGKGH
EATQEIMGKKRTFSDQDHARLALAARATHGKGGAE

Sequences:

>Translated_515_residues
MSAARSSHPAHQQIAAALAWLRQHVAPTAQLHADTRSLEAGDVFVAYAVDGADNRAFMADAVARGAAAVLYQPEGLAHAP
AAPAVPVALAVPALDQLAGEIASGWYGDPSDSLLAVGVTGTNGKTSCTQWIATALTALHQPCAVIGTLGTGMPGQLVPTG
FTTPDAPQLQRSLAQLRDAGAQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRA
AVINRDDAAGRRLLEKLAGRVRTIAYGIGDAPAPDADRELVALDVRATATGTAFRLRSSWGDANVEVGTLGTFNVSNLLA
VLGALLAADVPFDAALAEIERLESVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIAAARGGRLVCMFGCGG
DRDATKRPLMGAIAERLADEVVVTSDNPRSEDPQHIIDQVVAGMTAPDRARRIEDRASAILQAVRGAAREDVVVLAGKGH
EATQEIMGKKRTFSDQDHARLALAARATHGKGGAE
>Mature_514_residues
SAARSSHPAHQQIAAALAWLRQHVAPTAQLHADTRSLEAGDVFVAYAVDGADNRAFMADAVARGAAAVLYQPEGLAHAPA
APAVPVALAVPALDQLAGEIASGWYGDPSDSLLAVGVTGTNGKTSCTQWIATALTALHQPCAVIGTLGTGMPGQLVPTGF
TTPDAPQLQRSLAQLRDAGAQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRAA
VINRDDAAGRRLLEKLAGRVRTIAYGIGDAPAPDADRELVALDVRATATGTAFRLRSSWGDANVEVGTLGTFNVSNLLAV
LGALLAADVPFDAALAEIERLESVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIAAARGGRLVCMFGCGGD
RDATKRPLMGAIAERLADEVVVTSDNPRSEDPQHIIDQVVAGMTAPDRARRIEDRASAILQAVRGAAREDVVVLAGKGHE
ATQEIMGKKRTFSDQDHARLALAARATHGKGGAE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=471, Percent_Identity=44.7983014861996, Blast_Score=312, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54030; Mature: 53898

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARSSHPAHQQIAAALAWLRQHVAPTAQLHADTRSLEAGDVFVAYAVDGADNRAFMAD
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHH
AVARGAAAVLYQPEGLAHAPAAPAVPVALAVPALDQLAGEIASGWYGDPSDSLLAVGVTG
HHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
TNGKTSCTQWIATALTALHQPCAVIGTLGTGMPGQLVPTGFTTPDAPQLQRSLAQLRDAG
CCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCC
AQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRA
CCEEEHHHHHHHHHCCCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCEE
AVINRDDAAGRRLLEKLAGRVRTIAYGIGDAPAPDADRELVALDVRATATGTAFRLRSSW
EEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEECCC
GDANVEVGTLGTFNVSNLLAVLGALLAADVPFDAALAEIERLESVNGRMQRLGGRLQNDE
CCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PLVVIDYAHTPDALEKTLDALRPIAAARGGRLVCMFGCGGDRDATKRPLMGAIAERLADE
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCC
VVVTSDNPRSEDPQHIIDQVVAGMTAPDRARRIEDRASAILQAVRGAAREDVVVLAGKGH
EEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
EATQEIMGKKRTFSDQDHARLALAARATHGKGGAE
HHHHHHHCCCCCCCCCCHHEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SAARSSHPAHQQIAAALAWLRQHVAPTAQLHADTRSLEAGDVFVAYAVDGADNRAFMAD
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHH
AVARGAAAVLYQPEGLAHAPAAPAVPVALAVPALDQLAGEIASGWYGDPSDSLLAVGVTG
HHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
TNGKTSCTQWIATALTALHQPCAVIGTLGTGMPGQLVPTGFTTPDAPQLQRSLAQLRDAG
CCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCC
AQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRA
CCEEEHHHHHHHHHCCCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCEE
AVINRDDAAGRRLLEKLAGRVRTIAYGIGDAPAPDADRELVALDVRATATGTAFRLRSSW
EEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEECCC
GDANVEVGTLGTFNVSNLLAVLGALLAADVPFDAALAEIERLESVNGRMQRLGGRLQNDE
CCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PLVVIDYAHTPDALEKTLDALRPIAAARGGRLVCMFGCGGDRDATKRPLMGAIAERLADE
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCC
VVVTSDNPRSEDPQHIIDQVVAGMTAPDRARRIEDRASAILQAVRGAAREDVVVLAGKGH
EEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
EATQEIMGKKRTFSDQDHARLALAARATHGKGGAE
HHHHHHHCCCCCCCCCCHHEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]