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The map label for this gene is hemK [H]

Identifier: 116688533

GI number: 116688533

Start: 567563

End: 568405

Strand: Reverse

Name: hemK [H]

Synonym: Bcen2424_0509

Alternate gene names: 116688533

Gene position: 568405-567563 (Counterclockwise)

Preceding gene: 116688534

Following gene: 116688532

Centisome position: 16.32

GC content: 74.26

Gene sequence:

>843_bases
ATGTCCGACACCACCGCCGACGCCCTGCTGCGCGCCTCGCCGCTCGACCCGGTCGATGCACGCGTGCTGCTCGCGCACGC
GCTCGGCTGGACCCGCACGCAACTGATCACGCGCGGCGACGCGCCGCTCGACGCCGCCGCGGTCGAACGCTACCGCGCGC
TCGAAGCGCGCCGCGTCGCCGGCGAGCCCGTCGCGCAGCTGGTGGGGATGCGCGAGTTCTTCGGCCGCCCGTTCGACGTG
ACGCCCGACGTGCTGATCCCGCGCCCCGAAACCGAGCTGCTCGTCGAAGCCGCGCTCGATGCGATCGACGGGCTGCCGCA
CGCGGCCGTGCTCGATCTCGGCACCGGCAGCGGCGCGATCGCCGTGTCGATCGCGGCCGAGCGCCCCGATGCGCGCGTCT
GGGCGCTCGACCGTTCGCCGGCCGCGCTCGCGGTCGCGCAACGCAACGCCGACAAGCTGCTCGACGCGCACCGCCCCGGC
GGCCCGCTGCACTGGCTGCAGAGCGACTGGTACGCAGCGCTCGACCCCGCGCTCGGGTTCGACGCGATCGTCAGCAACCC
GCCGTACATCGCGCAGCACGATCCGCACCTCGCGCAAGGCGACCTGCGTTTCGAGCCGCGCGGCGCGCTCACCGACGACG
CCGATGGCCTCAGCGCGATCCGCGCGATCGTCGCGGGCGCCGGCGCCTACCTGAAACCGGGCGGCACGCTGTGGATCGAG
CACGGCTACGACCAGGCCGAAGCCGTCCGCGCGATACTGGCGTCACACGGCTTCGTCGCGGTCGAATCGCTCGCCGATCT
CGCCGCCATCGAACGCACGACCGGCGGCCGCCTGCCGGGCTGA

Upstream 100 bases:

>100_bases
GCGATCATGGACGGCGATCTCGACGAACTGATCGGCGCGCTCGTCACCGAGCACCAGGCCGAGTTGCTCGCGTCGCTCGG
CGAAGCCGACTAAGGCCACG

Downstream 100 bases:

>100_bases
TGCCCGGCCCGCCCGGTTGAAATCCGCTATCATTTCGATCTAACGCCCCGCAAACGCAAGGTCAGTCATGGACACCCAAC
AACGTATCAAGCAAATCGTC

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSDTTADALLRASPLDPVDARVLLAHALGWTRTQLITRGDAPLDAAAVERYRALEARRVAGEPVAQLVGMREFFGRPFDV
TPDVLIPRPETELLVEAALDAIDGLPHAAVLDLGTGSGAIAVSIAAERPDARVWALDRSPAALAVAQRNADKLLDAHRPG
GPLHWLQSDWYAALDPALGFDAIVSNPPYIAQHDPHLAQGDLRFEPRGALTDDADGLSAIRAIVAGAGAYLKPGGTLWIE
HGYDQAEAVRAILASHGFVAVESLADLAAIERTTGGRLPG

Sequences:

>Translated_280_residues
MSDTTADALLRASPLDPVDARVLLAHALGWTRTQLITRGDAPLDAAAVERYRALEARRVAGEPVAQLVGMREFFGRPFDV
TPDVLIPRPETELLVEAALDAIDGLPHAAVLDLGTGSGAIAVSIAAERPDARVWALDRSPAALAVAQRNADKLLDAHRPG
GPLHWLQSDWYAALDPALGFDAIVSNPPYIAQHDPHLAQGDLRFEPRGALTDDADGLSAIRAIVAGAGAYLKPGGTLWIE
HGYDQAEAVRAILASHGFVAVESLADLAAIERTTGGRLPG
>Mature_279_residues
SDTTADALLRASPLDPVDARVLLAHALGWTRTQLITRGDAPLDAAAVERYRALEARRVAGEPVAQLVGMREFFGRPFDVT
PDVLIPRPETELLVEAALDAIDGLPHAAVLDLGTGSGAIAVSIAAERPDARVWALDRSPAALAVAQRNADKLLDAHRPGG
PLHWLQSDWYAALDPALGFDAIVSNPPYIAQHDPHLAQGDLRFEPRGALTDDADGLSAIRAIVAGAGAYLKPGGTLWIEH
GYDQAEAVRAILASHGFVAVESLADLAAIERTTGGRLPG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=279, Percent_Identity=30.4659498207885, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1787463, Length=268, Percent_Identity=45.5223880597015, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI87082085, Length=201, Percent_Identity=37.8109452736318, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582226, Length=243, Percent_Identity=31.2757201646091, Blast_Score=107, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29576; Mature: 29444

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTTADALLRASPLDPVDARVLLAHALGWTRTQLITRGDAPLDAAAVERYRALEARRVA
CCCCHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHEEECCCCCCHHHHHHHHHHHHHHHHC
GEPVAQLVGMREFFGRPFDVTPDVLIPRPETELLVEAALDAIDGLPHAAVLDLGTGSGAI
CCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEE
AVSIAAERPDARVWALDRSPAALAVAQRNADKLLDAHRPGGPLHWLQSDWYAALDPALGF
EEEEECCCCCCEEEEECCCCCEEHHHHCCHHHHHHCCCCCCCCCHHHCCCHHHHCCCCCC
DAIVSNPPYIAQHDPHLAQGDLRFEPRGALTDDADGLSAIRAIVAGAGAYLKPGGTLWIE
HHHHCCCCEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCEECCCCEEEEE
HGYDQAEAVRAILASHGFVAVESLADLAAIERTTGGRLPG
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDTTADALLRASPLDPVDARVLLAHALGWTRTQLITRGDAPLDAAAVERYRALEARRVA
CCCHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHEEECCCCCCHHHHHHHHHHHHHHHHC
GEPVAQLVGMREFFGRPFDVTPDVLIPRPETELLVEAALDAIDGLPHAAVLDLGTGSGAI
CCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEE
AVSIAAERPDARVWALDRSPAALAVAQRNADKLLDAHRPGGPLHWLQSDWYAALDPALGF
EEEEECCCCCCEEEEECCCCCEEHHHHCCHHHHHHCCCCCCCCCHHHCCCHHHHCCCCCC
DAIVSNPPYIAQHDPHLAQGDLRFEPRGALTDDADGLSAIRAIVAGAGAYLKPGGTLWIE
HHHHCCCCEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCEECCCCEEEEE
HGYDQAEAVRAILASHGFVAVESLADLAAIERTTGGRLPG
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]