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The map label for this gene is prfA [H]

Identifier: 116688534

GI number: 116688534

Start: 568413

End: 569495

Strand: Reverse

Name: prfA [H]

Synonym: Bcen2424_0510

Alternate gene names: 116688534

Gene position: 569495-568413 (Counterclockwise)

Preceding gene: 116688535

Following gene: 116688533

Centisome position: 16.35

GC content: 66.2

Gene sequence:

>1083_bases
ATGAAGACGAGCATGCAACGCAAGCTCGACCAGCTGTCCACACGGCTGGCCGAACTGAACGACCTGCTGAGCCGCGAAAA
CGTCACGGCCGACCTCGACCAGTACCGCAAGCTGACGCGCGAACATGCGGAACTCGGCCCGGTCGTCGAGCAATACGCGT
TATGGCGCCAGTCGCGCAGCGACGAAACGGCCGCGCAGGAGCTGCTGGCCGATCCGTCGATGCGCGATTTCGCGGAAGAC
GAAATCCGCAGCGCGCGCGAGGGCATGGCGCGCCTCGAGACCGAGCTGCAGAAGATGCTGCTGCCGAAGGACCCGAACGA
CGACCGCAACATCTTCCTCGAAATCCGCGCGGGCACGGGCGGCGACGAATCGGCGCTGTTCGCGGGCGACCTGCTGCGCA
TGTACCTGCGCTTCGCGGAACGTCAGCGCTGGCAGGTCGAAATGATGTCGGAGAGCGCGTCGGATCTCGGCGGCTACAAG
GAAGTGATCGTGCGGATCGCGGGCCAGGGCGCGTATTCGCGCCTGAAGTTCGAATCGGGCGGCCATCGCGTACAGCGCGT
GCCGGCCACCGAGACGCAGGGGCGCATCCATACGTCCGCGTGCACGGTCGCCGTGATGCCGGAAGCCGACGAGATCGGCG
AGGTCGAGATCAATCCGGCCGACCTGCGGATCGACACGTTCCGCGCGTCCGGCGCGGGCGGTCAGCACATCAACAAGACC
GATTCGGCGGTGCGCGTCACGCACATCCCGACCGGGATCGTCGTCGAATGCCAGGACGACCGCTCGCAGCACAAGAACAA
GGATCGCGCGCTGAAGGTGCTCGCCGCGCGCATCAAGGACAAGCAGTATCACGAGCAGCACGCGAAGGAAGCCGCGACGC
GCAAGAGCCTGATCGGCTCCGGCGACCGCTCGGAGCGCATCCGCACGTACAACTTCCCGCAGGGCCGGATGACCGACCAC
CGGATCAACCTGACGCTGTACCGGCTCGAGGCGATCATGGACGGCGATCTCGACGAACTGATCGGCGCGCTCGTCACCGA
GCACCAGGCCGAGTTGCTCGCGTCGCTCGGCGAAGCCGACTAA

Upstream 100 bases:

>100_bases
CCACTAGCGCCGCCCGGTCGCCCTGCCGCCGGCCGCGCATTGTCCTGCCGCCGGCCTATCCTTTCCGAATACCCCTTGCC
CGGGAGCGCCGCTCCACACC

Downstream 100 bases:

>100_bases
GGCCACGATGTCCGACACCACCGCCGACGCCCTGCTGCGCGCCTCGCCGCTCGACCCGGTCGATGCACGCGTGCTGCTCG
CGCACGCGCTCGGCTGGACC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRSDETAAQELLADPSMRDFAED
EIRSAREGMARLETELQKMLLPKDPNDDRNIFLEIRAGTGGDESALFAGDLLRMYLRFAERQRWQVEMMSESASDLGGYK
EVIVRIAGQGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEAIMDGDLDELIGALVTEHQAELLASLGEAD

Sequences:

>Translated_360_residues
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRSDETAAQELLADPSMRDFAED
EIRSAREGMARLETELQKMLLPKDPNDDRNIFLEIRAGTGGDESALFAGDLLRMYLRFAERQRWQVEMMSESASDLGGYK
EVIVRIAGQGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEAIMDGDLDELIGALVTEHQAELLASLGEAD
>Mature_360_residues
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRSDETAAQELLADPSMRDFAED
EIRSAREGMARLETELQKMLLPKDPNDDRNIFLEIRAGTGGDESALFAGDLLRMYLRFAERQRWQVEMMSESASDLGGYK
EVIVRIAGQGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEAIMDGDLDELIGALVTEHQAELLASLGEAD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=340, Percent_Identity=45.2941176470588, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI34577120, Length=369, Percent_Identity=38.2113821138211, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI166795305, Length=245, Percent_Identity=42.0408163265306, Blast_Score=183, Evalue=2e-46,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=61.731843575419, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=39.2215568862275, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=295, Percent_Identity=37.6271186440678, Blast_Score=194, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=353, Percent_Identity=41.3597733711048, Blast_Score=249, Evalue=4e-67,
Organism=Drosophila melanogaster, GI19921226, Length=264, Percent_Identity=44.3181818181818, Blast_Score=221, Evalue=8e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40530; Mature: 40530

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DETAAQELLADPSMRDFAEDEIRSAREGMARLETELQKMLLPKDPNDDRNIFLEIRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAGDLLRMYLRFAERQRWQVEMMSESASDLGGYKEVIVRIAGQGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYRLEAIMDGDLDELIGALVTEHQAELLASLGEAD
CCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DETAAQELLADPSMRDFAEDEIRSAREGMARLETELQKMLLPKDPNDDRNIFLEIRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAGDLLRMYLRFAERQRWQVEMMSESASDLGGYKEVIVRIAGQGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYRLEAIMDGDLDELIGALVTEHQAELLASLGEAD
CCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA