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The map label for this gene is mlaE [H]

Identifier: 116688439

GI number: 116688439

Start: 467248

End: 468015

Strand: Direct

Name: mlaE [H]

Synonym: Bcen2424_0415

Alternate gene names: 116688439

Gene position: 467248-468015 (Clockwise)

Preceding gene: 116688438

Following gene: 116688440

Centisome position: 13.41

GC content: 66.41

Gene sequence:

>768_bases
ATGATCAGCGCGATCGGACGTTACGTCATCGGCGGCCTCGAGCGCGCGGGCTACGGCACGCGCCTGTTCGTGCGCCTCGT
GCTGGAATTCTTTCCGCTGCTGCGCCGGCCGCGGCTGGTCACGAAGCAGATCCACTTCCTCGGCAACTATTCGTTCGTGA
TCATTGCGGTGTCGGGGCTGTTCGTCGGCTTCGTGCTCGGCCTGCAGGGCTATTACACGCTGAATCGCTACGGTTCCGAG
CAGGCGCTCGGCCTGCTGGTCGCGCTGTCGCTCGTGCGCGAGCTCGGGCCGGTCGTCACCGCGCTGCTGTTCGCGGGCCG
CGCGGGCACGTCGCTCACGGCCGAGATCGGCCTGATGAAGGCCGGCGAGCAGCTCACCGCGCTCGAGATGATGGCGGTCG
ACCCGATCAAGAACGTGATCGCGCCGCGCATGTGGGCGGGCATCATCGCGATGCCGCTCCTGGCCGCGATCTTCAACGCG
GTCGGCGTGCTCGGCGGTTACTTCGTCGGCGTCGTGCTGATCGGCGTCGATCCGGGCGCGTTCTGGTCGCAGATGCAGGG
CGGGGTCCAGGTCTGGGCCGATGTCGGCAATGGCGTGATCAAGAGCATCGTGTTCGGCTTCGCCGTGACCTTCATTGCGC
TGTACCAGGGGTATGAAGCGAAGCCCACGCCCGAAGGCGTGTCGCGCGCGACCACCAAGACGGTCGTGTTCGCGTCGCTC
GCCGTACTCGGCCTCGATTTCCTGCTGACCGCGCTGATGTTCAGCTAA

Upstream 100 bases:

>100_bases
GCGTGCGGCAGTTCATCGACGGCGCGCCGGACGGCCCGTTCAAATTTCATTACACGAGCCCGCCGCTCGCAGCGGATTTC
GGGCTCGGCGGAGGGCGCGC

Downstream 100 bases:

>100_bases
GCCTCAGCCAAGCCAAATTTTGGAATGACGATGAAAAAGACTGCTCTCGACTTCTGGGTCGGCCTGTTCGTGGTGGTGGG
CTTCCTTGCGGTGCTGTTCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MISAIGRYVIGGLERAGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKNVIAPRMWAGIIAMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVQVWADVGNGVIKSIVFGFAVTFIALYQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS

Sequences:

>Translated_255_residues
MISAIGRYVIGGLERAGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKNVIAPRMWAGIIAMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVQVWADVGNGVIKSIVFGFAVTFIALYQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS
>Mature_255_residues
MISAIGRYVIGGLERAGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKNVIAPRMWAGIIAMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVQVWADVGNGVIKSIVFGFAVTFIALYQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=220, Percent_Identity=60.9090909090909, Blast_Score=268, Evalue=4e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27232; Mature: 27232

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCC
>Mature Secondary Structure
MISAIGRYVIGGLERAGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGL
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHH
FVGFVLGLQGYYTLNRYGSEQALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMK
HHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AGEQLTALEMMAVDPIKNVIAPRMWAGIIAMPLLAAIFNAVGVLGGYFVGVVLIGVDPGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
FWSQMQGGVQVWADVGNGVIKSIVFGFAVTFIALYQGYEAKPTPEGVSRATTKTVVFASL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AVLGLDFLLTALMFS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]