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The map label for this gene is rhtB [H]

Identifier: 116688332

GI number: 116688332

Start: 348829

End: 349464

Strand: Direct

Name: rhtB [H]

Synonym: Bcen2424_0308

Alternate gene names: 116688332

Gene position: 348829-349464 (Clockwise)

Preceding gene: 116688331

Following gene: 116688334

Centisome position: 10.01

GC content: 68.71

Gene sequence:

>636_bases
ATGAACTTGCATACGTGGTGGCTTTTCGTGGCGACGGTGTTCGTCGTGTCGGCGATTCCGGGCCCGAACATGCTGCTCGT
GATGACGCACGGCGCGCGGCACGGGCTGCGGCGCTCGTCCGCGACGATGGCCGGTTGCCTGAGCGCGCTGGTGCTGATGC
TCGCGGTGTCCGCGGCGGGGCTCGGCGCGGTGCTCGAGGCATGGCCGGCGATGTTCAATACGTTGCGCTTCGCGGGCGCG
GCCTACCTGGTCTATCTCGGCGTGAAGGCGTGGCGCGCCCGCGTGGACGACGCGCCGGCGGCCGACGTCGACGCCGTGTC
GCAAGGCGCATCGGCCTCGCGCGCGGTGTTGTTCCGCAATGGCTTCCTGGTCGCGGGCAGCAACCCGAAGGCGATCCTGT
TCGCGGCCGCGCTGCTGCCGCAGTTCATCAACGCGGCCGAGCCGACGCTGCCGCAGTTCGGCATTCTCGTCGTCACGTTC
GCGGTGATCGAGGTGAGCTGGTATCTCGTCTACGCGTCGTTCGGCACGCGCATCGGCGCCACGCTGAAGAGCCAGAGCGT
CGCGAGGGTGTTCAACCGTCTGACGGGCGGGCTGTTTGTCGGCTTCGGCGCGATGATGGCGCTGGTTCGGCACTGA

Upstream 100 bases:

>100_bases
GTCGCGCTGCGCCGCGCCGGCCGCTGACACGCGACAGAAGCGCGACCCGCACGGCATAATCGGCGGTTCGTTTCCCCCTA
CATTTGAGGCAGAGGCAGTC

Downstream 100 bases:

>100_bases
TTCCCGGCCGACACCGGCGTACCGAACGCCCCGTCCCTCGCGAGAGGGTGGGGCGTTCGGCTTTTTGGCGCCGCTTCATC
GTCGCTTCACCGCCGCTGGC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSSATMAGCLSALVLMLAVSAAGLGAVLEAWPAMFNTLRFAGA
AYLVYLGVKAWRARVDDAPAADVDAVSQGASASRAVLFRNGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGILVVTF
AVIEVSWYLVYASFGTRIGATLKSQSVARVFNRLTGGLFVGFGAMMALVRH

Sequences:

>Translated_211_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSSATMAGCLSALVLMLAVSAAGLGAVLEAWPAMFNTLRFAGA
AYLVYLGVKAWRARVDDAPAADVDAVSQGASASRAVLFRNGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGILVVTF
AVIEVSWYLVYASFGTRIGATLKSQSVARVFNRLTGGLFVGFGAMMALVRH
>Mature_211_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSSATMAGCLSALVLMLAVSAAGLGAVLEAWPAMFNTLRFAGA
AYLVYLGVKAWRARVDDAPAADVDAVSQGASASRAVLFRNGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGILVVTF
AVIEVSWYLVYASFGTRIGATLKSQSVARVFNRLTGGLFVGFGAMMALVRH

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=211, Percent_Identity=34.5971563981043, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1788099, Length=213, Percent_Identity=30.5164319248826, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22359; Mature: 22359

Theoretical pI: Translated: 11.07; Mature: 11.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSSATMAGCLSALVLMLAVSAAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LGAVLEAWPAMFNTLRFAGAAYLVYLGVKAWRARVDDAPAADVDAVSQGASASRAVLFRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEC
GFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGILVVTFAVIEVSWYLVYASFGTRIGA
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLKSQSVARVFNRLTGGLFVGFGAMMALVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSSATMAGCLSALVLMLAVSAAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LGAVLEAWPAMFNTLRFAGAAYLVYLGVKAWRARVDDAPAADVDAVSQGASASRAVLFRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEC
GFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGILVVTFAVIEVSWYLVYASFGTRIGA
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLKSQSVARVFNRLTGGLFVGFGAMMALVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]