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The map label for this gene is ywqM [H]

Identifier: 116688334

GI number: 116688334

Start: 351028

End: 351906

Strand: Direct

Name: ywqM [H]

Synonym: Bcen2424_0310

Alternate gene names: 116688334

Gene position: 351028-351906 (Clockwise)

Preceding gene: 116688332

Following gene: 116688342

Centisome position: 10.08

GC content: 73.72

Gene sequence:

>879_bases
ATGGTCGCCGCGGCCGCGAAGCTCCACCTGACGCAGCCGGGCCTGACGCGGCGCGTGCAGAACCTCGAAACCTTGCTCGG
CATGCCGCTGCTCGAACGGCAGAGCAAGCCGCTGAAGCCGACCGCGGCCGGACGCGACGTCTACGCGCTCGCGCGCAACG
TGCTCGGCGCCGTCGACGAACTGATGGCGGCGGGCGCGCCGGACAGCGAGCCGTCGGGTGAGCTGCGCATCGGCGTGCCG
CCGTTCCTGTCGGAGCTCGCGCTGGAGCGGCCGATCGACCGGCTGCGCGACGCGTTTCCGCGCCTCACGCTGCGCGTGAC
GGCCGGCTGGTCGCCGGCGCTGGTGCAGGGCATCGAGCGCGGCGCGCTCGACGTCGCGACCGTGATGGTGCCGGCGAGCG
CGGCGCTGCCGGACGCGTTGACGGCGACGCTGCTCGCCACGCAGCCGACGGTGCTCGTCGCCGCGCGCGATTTCCCGCTG
CCGGACGGCCCGCTGTCGCTCGATACGCTGTCCGGTTATCCGTGGGTGCTGAGCCAGGACGGCTGCGGAATGCGTTCGGC
GCTGAGCCGCGCGCTCGGCGCGGCCGGGCTGCCGTTCGACGTCGCGGTCGAGGCATTCGGCTCGGAGTTGCAGTTGTCGC
TCGTCGCGCGCGGCGCCGGCATCGGCATCGCGCCGCCGAACGCGCTGGCGCGCAGCGCGCATCGCGAGGCGCTGAGGGTC
GTGGAAACGGCGGGGCTGGAAACGCGGATCAACGTGTGGCTCGTGCACGGCACGTTGCCGGGCCGGCTGGTGCGGCCCGT
CGCGCTGCTGCGCGATGCGCTGGGCGAGGTGCTCGAGCGGGAGAATGGAAAGGGGTCAGGTGCCGTCACGGCGAACTAG

Upstream 100 bases:

>100_bases
TGTTTATGCGACAATTGAAATATGTCTAACATGATTATGCGAGTTTGTTTTGAATACGCGTGATCTCCAGGCGTTCGTTG
CGGTCGTCGACAGCGGGTCG

Downstream 100 bases:

>100_bases
AGGCCGCATTCGAGGGAAAATCCTTATTTTCTCTACGGAAATGTTGCATTGCGGAAACCGATTCGATATGATTGGAACTG
TCTCCTCCATGTCTCCTCTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MVAAAAKLHLTQPGLTRRVQNLETLLGMPLLERQSKPLKPTAAGRDVYALARNVLGAVDELMAAGAPDSEPSGELRIGVP
PFLSELALERPIDRLRDAFPRLTLRVTAGWSPALVQGIERGALDVATVMVPASAALPDALTATLLATQPTVLVAARDFPL
PDGPLSLDTLSGYPWVLSQDGCGMRSALSRALGAAGLPFDVAVEAFGSELQLSLVARGAGIGIAPPNALARSAHREALRV
VETAGLETRINVWLVHGTLPGRLVRPVALLRDALGEVLERENGKGSGAVTAN

Sequences:

>Translated_292_residues
MVAAAAKLHLTQPGLTRRVQNLETLLGMPLLERQSKPLKPTAAGRDVYALARNVLGAVDELMAAGAPDSEPSGELRIGVP
PFLSELALERPIDRLRDAFPRLTLRVTAGWSPALVQGIERGALDVATVMVPASAALPDALTATLLATQPTVLVAARDFPL
PDGPLSLDTLSGYPWVLSQDGCGMRSALSRALGAAGLPFDVAVEAFGSELQLSLVARGAGIGIAPPNALARSAHREALRV
VETAGLETRINVWLVHGTLPGRLVRPVALLRDALGEVLERENGKGSGAVTAN
>Mature_292_residues
MVAAAAKLHLTQPGLTRRVQNLETLLGMPLLERQSKPLKPTAAGRDVYALARNVLGAVDELMAAGAPDSEPSGELRIGVP
PFLSELALERPIDRLRDAFPRLTLRVTAGWSPALVQGIERGALDVATVMVPASAALPDALTATLLATQPTVLVAARDFPL
PDGPLSLDTLSGYPWVLSQDGCGMRSALSRALGAAGLPFDVAVEAFGSELQLSLVARGAGIGIAPPNALARSAHREALRV
VETAGLETRINVWLVHGTLPGRLVRPVALLRDALGEVLERENGKGSGAVTAN

Specific function: This Protein Is A Positive Regulator Of Gene Expression For The Cysteine Regulon, A System Of 10 Or More Loci Involved In The Biosynthesis Of L-Cysteine From Inorganic Sulfate. The Inducer For Cysb Is N-Acetylserine. Cysb Inhibits Its Own Transcription.

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=224, Percent_Identity=26.3392857142857, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=27.0588235294118, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787879, Length=259, Percent_Identity=27.027027027027, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=219, Percent_Identity=26.4840182648402, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30516; Mature: 30516

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAAAAKLHLTQPGLTRRVQNLETLLGMPLLERQSKPLKPTAAGRDVYALARNVLGAVDE
CCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LMAAGAPDSEPSGELRIGVPPFLSELALERPIDRLRDAFPRLTLRVTAGWSPALVQGIER
HHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHC
GALDVATVMVPASAALPDALTATLLATQPTVLVAARDFPLPDGPLSLDTLSGYPWVLSQD
CCHHHHHHEECCHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHCCCCCCEEECCC
GCGMRSALSRALGAAGLPFDVAVEAFGSELQLSLVARGAGIGIAPPNALARSAHREALRV
CCCHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
VETAGLETRINVWLVHGTLPGRLVRPVALLRDALGEVLERENGKGSGAVTAN
HHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECC
>Mature Secondary Structure
MVAAAAKLHLTQPGLTRRVQNLETLLGMPLLERQSKPLKPTAAGRDVYALARNVLGAVDE
CCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LMAAGAPDSEPSGELRIGVPPFLSELALERPIDRLRDAFPRLTLRVTAGWSPALVQGIER
HHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHC
GALDVATVMVPASAALPDALTATLLATQPTVLVAARDFPLPDGPLSLDTLSGYPWVLSQD
CCHHHHHHEECCHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHCCCCCCEEECCC
GCGMRSALSRALGAAGLPFDVAVEAFGSELQLSLVARGAGIGIAPPNALARSAHREALRV
CCCHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
VETAGLETRINVWLVHGTLPGRLVRPVALLRDALGEVLERENGKGSGAVTAN
HHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]