| Definition | Burkholderia cenocepacia HI2424 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008542 |
| Length | 3,483,902 |
Click here to switch to the map view.
The map label for this gene is pstIM [H]
Identifier: 116688241
GI number: 116688241
Start: 244043
End: 245779
Strand: Direct
Name: pstIM [H]
Synonym: Bcen2424_0217
Alternate gene names: 116688241
Gene position: 244043-245779 (Clockwise)
Preceding gene: 116688240
Following gene: 116688243
Centisome position: 7.0
GC content: 59.12
Gene sequence:
>1737_bases ATGACGCCAACCACAAAGTCCCAGTTCCTCCGCCTCGTCCGCGCTCTTCGGGCTGTCATGCAAGGGGCGCAACTCGGAAC AGATGAGGTCATAGGTAGCGCATTAATGCGCTGGTGCAAGGAGTCGTTCCCGGGGTTGCCGCGTTCCGGCCTGCCTGTGA ATGCGCAATTACGGAATGCCCCGGCCGTCGCTGCATTCGTCGACTTCGTGAAGAAAAAGGACCTTCTTGAGGCGATTTAC TGGCTCTCCTCGGCCTATGCTCAGCTCGCTGGCGAAGAGCGCCGTAAGCAACTGGCGATGTTCTTTACGCCGCCATCATT GACCAAACGTCTGCTGGATGACTTGTCCGCGAGTGGTGTGGATTTTTCGGTCCGGAAGTTCTGTGACCCCGCGTGTGGGG GCGCCGCGTTCCTAGCGCCAATTGCGATGCGCATGCGCGATGCATTGCGCGAGCGAGGAACATCCGCGACGCAAATTCTT GACCACGTACAGCGCCACCTCCTCGGATTCGATAAGGATGCTGCTCTGTGCGAGATGTCAAAGCACTTCCTGCTGATGGT TCTGCATGACGAAGTTGTCGCGACTGGTGCGCGACCGAAGTTCCAGATTCACCAAGGCGATTCGCTGATACGAGCCCAGT CACTGCTCGGGGCTCTCGACGTTGTGGTTTGCAACCCACCGTTCCGGAAGATGCCGTCCGCCGAGGTCGCGCATTATCTA GAGCACTTCGCGGACATCATCGAAGCGCAGCCGAATTTGTACGCACTTTTCATGGCCTTATGCGTCAAATTGCTGGCGCC GGGGGGAACCTGCGCGCTTGTAACGCCGACAAGCTTCCTGTCCGGCCAATACTTTTCGAAGCTGCGCACGTTCTTGTTGA CCCAAGCCAACGTGTTGAGTATCGGCATGGTTAGCGACCGGCTCGGCGTATTCATCGACGTAGAGCAGGAAACAGCACTG ACGCTGGCGCGGCGAGAACAGACTGGTCATGGGCCCGGTACCGACGCTTACGTGTCGGTTGTTGCCCGTGACGGCAACTA CGTCAACGTGGGTCGTTGCGTCCTTCCTAACTCGGGCGCCGCGTGGCCTGTTCCGCGCACGGAGAGCGACGTTGTATTGC TCAAAACGGCCGCAAAATCACGTGCAACCCTTGCCGACTATGGATACACCCCCCGTATTGGCGCATTCGTGTGGAACCGG GACACGCGCACGACGTACCCATCAGCGAAGAGTGCCGCACGTGCGCATGGAGGAACCGCTGTCCCGCTGCTGTGGTCAAG CGATATTGCTCAAGATGGTTCTCTGCGTTTCTCCGGCGCACCGAAAGCGAACCGGGAACACTGCTTCGTGAATCTCGGGG CGAAAGACCACCGCTCGGTTGTCCGTCGGCCTAGCGTCTTGCTGCAGCGTGTTACCTCTAACGACCAGCCTCGACGCCTA GTTGCTGCAGCCGTACCGCCAGCACTAATAGAAACCTATGGTGGTTTCGTCGGCGAGAACCACACCGTCATCCTCGAACA GGTACATCCTGAGCCCGCGCTCGCCCCCGAGGAACTGGCGCAATTACTCGCCACGCCCACGGTGGACCGCTACTTTCGTT GTATTTCTGGGGCAACAAACGTATCCATTTTCGAGCTCGGCCAGCTTCGACTGCCCGACCCGGGACGGCTCAGAAATCTC CTCGCGCGGGGATGCGACATGCCGAGTGCAGCGCGCAAAGCGCTTCAAGAGCAGTAG
Upstream 100 bases:
>100_bases GCGACATGCGATGAGCTTGTGGTCAAGGCGCCCGAGTCCGCTCTGAAAGCCATTAAAGACCTAGACCGATGCAGCGAGCA GACTCGATAGCCACCTGCCA
Downstream 100 bases:
>100_bases TCCGTTTTTGCCCATACGCCGGGAATCGCGACAAACATAAGACTCATGGCTTCGAGAAAACATCTCACCGACCTGCTAGA GGCGCAGAAGAAGCGGCGCG
Product: N-6 DNA methylase
Products: NA
Alternate protein names: M.PstI; Adenine-specific methyltransferase PstI [H]
Number of amino acids: Translated: 578; Mature: 577
Protein sequence:
>578_residues MTPTTKSQFLRLVRALRAVMQGAQLGTDEVIGSALMRWCKESFPGLPRSGLPVNAQLRNAPAVAAFVDFVKKKDLLEAIY WLSSAYAQLAGEERRKQLAMFFTPPSLTKRLLDDLSASGVDFSVRKFCDPACGGAAFLAPIAMRMRDALRERGTSATQIL DHVQRHLLGFDKDAALCEMSKHFLLMVLHDEVVATGARPKFQIHQGDSLIRAQSLLGALDVVVCNPPFRKMPSAEVAHYL EHFADIIEAQPNLYALFMALCVKLLAPGGTCALVTPTSFLSGQYFSKLRTFLLTQANVLSIGMVSDRLGVFIDVEQETAL TLARREQTGHGPGTDAYVSVVARDGNYVNVGRCVLPNSGAAWPVPRTESDVVLLKTAAKSRATLADYGYTPRIGAFVWNR DTRTTYPSAKSAARAHGGTAVPLLWSSDIAQDGSLRFSGAPKANREHCFVNLGAKDHRSVVRRPSVLLQRVTSNDQPRRL VAAAVPPALIETYGGFVGENHTVILEQVHPEPALAPEELAQLLATPTVDRYFRCISGATNVSIFELGQLRLPDPGRLRNL LARGCDMPSAARKALQEQ
Sequences:
>Translated_578_residues MTPTTKSQFLRLVRALRAVMQGAQLGTDEVIGSALMRWCKESFPGLPRSGLPVNAQLRNAPAVAAFVDFVKKKDLLEAIY WLSSAYAQLAGEERRKQLAMFFTPPSLTKRLLDDLSASGVDFSVRKFCDPACGGAAFLAPIAMRMRDALRERGTSATQIL DHVQRHLLGFDKDAALCEMSKHFLLMVLHDEVVATGARPKFQIHQGDSLIRAQSLLGALDVVVCNPPFRKMPSAEVAHYL EHFADIIEAQPNLYALFMALCVKLLAPGGTCALVTPTSFLSGQYFSKLRTFLLTQANVLSIGMVSDRLGVFIDVEQETAL TLARREQTGHGPGTDAYVSVVARDGNYVNVGRCVLPNSGAAWPVPRTESDVVLLKTAAKSRATLADYGYTPRIGAFVWNR DTRTTYPSAKSAARAHGGTAVPLLWSSDIAQDGSLRFSGAPKANREHCFVNLGAKDHRSVVRRPSVLLQRVTSNDQPRRL VAAAVPPALIETYGGFVGENHTVILEQVHPEPALAPEELAQLLATPTVDRYFRCISGATNVSIFELGQLRLPDPGRLRNL LARGCDMPSAARKALQEQ >Mature_577_residues TPTTKSQFLRLVRALRAVMQGAQLGTDEVIGSALMRWCKESFPGLPRSGLPVNAQLRNAPAVAAFVDFVKKKDLLEAIYW LSSAYAQLAGEERRKQLAMFFTPPSLTKRLLDDLSASGVDFSVRKFCDPACGGAAFLAPIAMRMRDALRERGTSATQILD HVQRHLLGFDKDAALCEMSKHFLLMVLHDEVVATGARPKFQIHQGDSLIRAQSLLGALDVVVCNPPFRKMPSAEVAHYLE HFADIIEAQPNLYALFMALCVKLLAPGGTCALVTPTSFLSGQYFSKLRTFLLTQANVLSIGMVSDRLGVFIDVEQETALT LARREQTGHGPGTDAYVSVVARDGNYVNVGRCVLPNSGAAWPVPRTESDVVLLKTAAKSRATLADYGYTPRIGAFVWNRD TRTTYPSAKSAARAHGGTAVPLLWSSDIAQDGSLRFSGAPKANREHCFVNLGAKDHRSVVRRPSVLLQRVTSNDQPRRLV AAAVPPALIETYGGFVGENHTVILEQVHPEPALAPEELAQLLATPTVDRYFRCISGATNVSIFELGQLRLPDPGRLRNLL ARGCDMPSAARKALQEQ
Specific function: This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PstI endonuclease [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002296 - InterPro: IPR011639 [H]
Pfam domain/function: PF07669 Eco57I [H]
EC number: =2.1.1.72 [H]
Molecular weight: Translated: 63042; Mature: 62911
Theoretical pI: Translated: 9.23; Mature: 9.23
Prosite motif: PS00092 N6_MTASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.9 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 1.9 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 3.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTPTTKSQFLRLVRALRAVMQGAQLGTDEVIGSALMRWCKESFPGLPRSGLPVNAQLRNA CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCC PAVAAFVDFVKKKDLLEAIYWLSSAYAQLAGEERRKQLAMFFTPPSLTKRLLDDLSASGV CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHCCCCC DFSVRKFCDPACGGAAFLAPIAMRMRDALRERGTSATQILDHVQRHLLGFDKDAALCEMS CHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHH KHFLLMVLHDEVVATGARPKFQIHQGDSLIRAQSLLGALDVVVCNPPFRKMPSAEVAHYL HHEEEEEEEHHHHHCCCCCCEEEECCCHHHHHHHHHHHHEEEEECCCHHHCCHHHHHHHH EHFADIIEAQPNLYALFMALCVKLLAPGGTCALVTPTSFLSGQYFSKLRTFLLTQANVLS HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEE IGMVSDRLGVFIDVEQETALTLARREQTGHGPGTDAYVSVVARDGNYVNVGRCVLPNSGA EEEECCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEECEEEECCCCC AWPVPRTESDVVLLKTAAKSRATLADYGYTPRIGAFVWNRDTRTTYPSAKSAARAHGGTA CCCCCCCCCCEEEEEECCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCE VPLLWSSDIAQDGSLRFSGAPKANREHCFVNLGAKDHRSVVRRPSVLLQRVTSNDQPRRL EEEEECCCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHH VAAAVPPALIETYGGFVGENHTVILEQVHPEPALAPEELAQLLATPTVDRYFRCISGATN HHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCC VSIFELGQLRLPDPGRLRNLLARGCDMPSAARKALQEQ EEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCC >Mature Secondary Structure TPTTKSQFLRLVRALRAVMQGAQLGTDEVIGSALMRWCKESFPGLPRSGLPVNAQLRNA CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCC PAVAAFVDFVKKKDLLEAIYWLSSAYAQLAGEERRKQLAMFFTPPSLTKRLLDDLSASGV CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHCCCCC DFSVRKFCDPACGGAAFLAPIAMRMRDALRERGTSATQILDHVQRHLLGFDKDAALCEMS CHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHH KHFLLMVLHDEVVATGARPKFQIHQGDSLIRAQSLLGALDVVVCNPPFRKMPSAEVAHYL HHEEEEEEEHHHHHCCCCCCEEEECCCHHHHHHHHHHHHEEEEECCCHHHCCHHHHHHHH EHFADIIEAQPNLYALFMALCVKLLAPGGTCALVTPTSFLSGQYFSKLRTFLLTQANVLS HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEE IGMVSDRLGVFIDVEQETALTLARREQTGHGPGTDAYVSVVARDGNYVNVGRCVLPNSGA EEEECCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEECEEEECCCCC AWPVPRTESDVVLLKTAAKSRATLADYGYTPRIGAFVWNRDTRTTYPSAKSAARAHGGTA CCCCCCCCCCEEEEEECCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCE VPLLWSSDIAQDGSLRFSGAPKANREHCFVNLGAKDHRSVVRRPSVLLQRVTSNDQPRRL EEEEECCCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHH VAAAVPPALIETYGGFVGENHTVILEQVHPEPALAPEELAQLLATPTVDRYFRCISGATN HHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCC VSIFELGQLRLPDPGRLRNLLARGCDMPSAARKALQEQ EEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 6330092 [H]