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The map label for this gene is caiD [H]

Identifier: 116688201

GI number: 116688201

Start: 195917

End: 196720

Strand: Direct

Name: caiD [H]

Synonym: Bcen2424_0177

Alternate gene names: 116688201

Gene position: 195917-196720 (Clockwise)

Preceding gene: 116688200

Following gene: 116688203

Centisome position: 5.62

GC content: 63.56

Gene sequence:

>804_bases
ATGAGCGCCATTCTCTCACCGCTTCTCTACGAAAAGCGCGCGCGCGTCGCCACCTTGACGATCAACCGGCCAGATGTGAT
GAACGCGATGGACTCGCACGCGCACCTTGCGCTCAGCGAGGCACTCGACGAAGCCCAGCATGATCCCGAGGTTCGCGTGG
TCGTGCTCACCGGATGCGGCGACCGGGCTTTTTCCGCAGGCCGCGATCTGCGGGAACTCGCGCGTCAAGCCGACCTGAAT
CCGGAAGAAAAGGCCGCGCTCGATCAGCGTTGGGCCCGCACACGCAGGCTGACGGATCGCCAAGATTTCTTCAAGCCGAT
CATTGCGCGCGTGAACGGCGTGGCGCTGGGCGGCGGCTTCGAGCTTGCTCTCGCTTGCGATCTGATTGTCGCGGCCGACC
ACGCTACCTTTGCGCTGCCAGAACCGAAGCGCGGACTCATCGCCACGGCCGGTGGCGTGCATCGGCTGCCGCGCCAGTTG
CCGCTAAAGATTGCGATGGGATTATTGCTGACCGGCGATCTGATAACCGCGCCACGCGCCGCCGAGCTTGGACTTGTGAA
CGAATGCGTGCCCGCCGCCGAACTCAATCTTGCCGTCACCCGTTATGTGGATCGTTTGCTCGCCTGTGCGCCGCTTTCCG
TGCAGGCCACGAAGCAATGCGCGATGAACGGCCTGGGCCGCACGCTGCAAGAAGCGCTGACCGCGCACTATCCGCTCGAA
GCGCTGCGTCAGTCGAGCGAAGATGCCCTTGAAGGGCCAAGGGCGTTCGCCGAGAAACGCACACCGCATTGGAAGGGATG
TTGA

Upstream 100 bases:

>100_bases
CGAGCGCTTCGCATGCGGCCGCTTTCGAACGGGAAACGGCCGAACAGAAGTGGTCAATGCAGCAACCCGAGTTTCTCTCG
ATGCTGGGGAGCGCGCAAAA

Downstream 100 bases:

>100_bases
CATGACAAACCGTTCGCGCAGGGCTGTCGCGTCAGCGGCCTTTCTTCTCGATCGCTCGAAACGGCAGGTCGAGCAAGCGC
GCGAGTTGTTCCGCGCCAGC

Product: enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSAILSPLLYEKRARVATLTINRPDVMNAMDSHAHLALSEALDEAQHDPEVRVVVLTGCGDRAFSAGRDLRELARQADLN
PEEKAALDQRWARTRRLTDRQDFFKPIIARVNGVALGGGFELALACDLIVAADHATFALPEPKRGLIATAGGVHRLPRQL
PLKIAMGLLLTGDLITAPRAAELGLVNECVPAAELNLAVTRYVDRLLACAPLSVQATKQCAMNGLGRTLQEALTAHYPLE
ALRQSSEDALEGPRAFAEKRTPHWKGC

Sequences:

>Translated_267_residues
MSAILSPLLYEKRARVATLTINRPDVMNAMDSHAHLALSEALDEAQHDPEVRVVVLTGCGDRAFSAGRDLRELARQADLN
PEEKAALDQRWARTRRLTDRQDFFKPIIARVNGVALGGGFELALACDLIVAADHATFALPEPKRGLIATAGGVHRLPRQL
PLKIAMGLLLTGDLITAPRAAELGLVNECVPAAELNLAVTRYVDRLLACAPLSVQATKQCAMNGLGRTLQEALTAHYPLE
ALRQSSEDALEGPRAFAEKRTPHWKGC
>Mature_266_residues
SAILSPLLYEKRARVATLTINRPDVMNAMDSHAHLALSEALDEAQHDPEVRVVVLTGCGDRAFSAGRDLRELARQADLNP
EEKAALDQRWARTRRLTDRQDFFKPIIARVNGVALGGGFELALACDLIVAADHATFALPEPKRGLIATAGGVHRLPRQLP
LKIAMGLLLTGDLITAPRAAELGLVNECVPAAELNLAVTRYVDRLLACAPLSVQATKQCAMNGLGRTLQEALTAHYPLEA
LRQSSEDALEGPRAFAEKRTPHWKGC

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=33.7164750957854, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI20127408, Length=184, Percent_Identity=33.695652173913, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI31542718, Length=228, Percent_Identity=31.140350877193, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI70995211, Length=259, Percent_Identity=28.1853281853282, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4502327, Length=262, Percent_Identity=30.5343511450382, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=29.2307692307692, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI37594471, Length=195, Percent_Identity=29.2307692307692, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI45643119, Length=204, Percent_Identity=28.921568627451, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260275230, Length=204, Percent_Identity=28.921568627451, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260274832, Length=204, Percent_Identity=28.921568627451, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI68989263, Length=191, Percent_Identity=29.3193717277487, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=39.4736842105263, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI1787659, Length=263, Percent_Identity=38.7832699619772, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1788597, Length=271, Percent_Identity=31.3653136531365, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=31.1538461538462, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=33.7254901960784, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788682, Length=179, Percent_Identity=30.7262569832402, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=32.5966850828729, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=36.8, Blast_Score=150, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=35.4330708661417, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17540714, Length=207, Percent_Identity=35.2657004830918, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17549921, Length=226, Percent_Identity=30.5309734513274, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=185, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=248, Percent_Identity=28.2258064516129, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=255, Percent_Identity=25.0980392156863, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=195, Percent_Identity=29.2307692307692, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=195, Percent_Identity=29.2307692307692, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=195, Percent_Identity=29.2307692307692, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=193, Percent_Identity=26.9430051813472, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=193, Percent_Identity=26.9430051813472, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=155, Percent_Identity=32.258064516129, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=215, Percent_Identity=26.046511627907, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=222, Percent_Identity=26.1261261261261, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=198, Percent_Identity=26.7676767676768, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=37.3983739837398, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=37.3983739837398, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=32.9545454545455, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=27.906976744186, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357171, Length=186, Percent_Identity=32.7956989247312, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24650670, Length=184, Percent_Identity=30.4347826086957, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920382, Length=232, Percent_Identity=28.448275862069, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571730, Length=167, Percent_Identity=29.940119760479, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571729, Length=167, Percent_Identity=29.940119760479, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24654903, Length=188, Percent_Identity=27.6595744680851, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 28975; Mature: 28844

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAILSPLLYEKRARVATLTINRPDVMNAMDSHAHLALSEALDEAQHDPEVRVVVLTGCG
CCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
DRAFSAGRDLRELARQADLNPEEKAALDQRWARTRRLTDRQDFFKPIIARVNGVALGGGF
CHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEECCCH
ELALACDLIVAADHATFALPEPKRGLIATAGGVHRLPRQLPLKIAMGLLLTGDLITAPRA
HHHHHHHHHHCCCCCEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCH
AELGLVNECVPAAELNLAVTRYVDRLLACAPLSVQATKQCAMNGLGRTLQEALTAHYPLE
HHHCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
ALRQSSEDALEGPRAFAEKRTPHWKGC
HHHCCCHHHHHCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SAILSPLLYEKRARVATLTINRPDVMNAMDSHAHLALSEALDEAQHDPEVRVVVLTGCG
CHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
DRAFSAGRDLRELARQADLNPEEKAALDQRWARTRRLTDRQDFFKPIIARVNGVALGGGF
CHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEECCCH
ELALACDLIVAADHATFALPEPKRGLIATAGGVHRLPRQLPLKIAMGLLLTGDLITAPRA
HHHHHHHHHHCCCCCEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCH
AELGLVNECVPAAELNLAVTRYVDRLLACAPLSVQATKQCAMNGLGRTLQEALTAHYPLE
HHHCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
ALRQSSEDALEGPRAFAEKRTPHWKGC
HHHCCCHHHHHCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA