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The map label for this gene is echA14 [H]

Identifier: 116688200

GI number: 116688200

Start: 195174

End: 195920

Strand: Direct

Name: echA14 [H]

Synonym: Bcen2424_0176

Alternate gene names: 116688200

Gene position: 195174-195920 (Clockwise)

Preceding gene: 116688199

Following gene: 116688201

Centisome position: 5.6

GC content: 64.39

Gene sequence:

>747_bases
ATGAAATGCTATGTAACCGCAATCCACGAGAACGGGATCGCCACGCTCACGCTTGCCGATCCCGAGCGTCGTAATGTCCT
TTCCGAGGTGCTTTGCGAACAGTTGATCGATGCGGTCGCGGCGGCTCATGCCGACCAGGATGTCCGCGCGCTGGTGATTG
CTGCGCAGGGTCCAGCGTTCTGCGCGGGCGCGCATCGCGACGATCTGCGCGCCGCCGCCGAAGGTGATGCACGCGTGATC
GGCAAGGTGTATCAAACCTTCATGGACGTCGCGAATTCGCCGCTGCCCACCATCGCAGCGATCAACGGGCCGGCAGTCGG
CGCCGGCATGAACCTGGCGCTCGCATGCGACTTGCGCATTGCGTCGTCGTCCGCCCGCTTCGATACGCGCTTTATCGGTA
TCGGCTTGCACCCCGGCGGTGGGCATGGCTGGATGCTTGTGCGTGCAGTGGGATGGCAAAACGCAGCCAGTCTGCTGCTG
CTCGGCGCAGCCGTGAATGCGGCGGAAGCGATGCGCATGGGTCTGGTGAGCGCCTGCGTGGCCGATGACGGCCTGGCCGA
CGCCGTCGCAAGCGCCTGCCGACGCATTCGTAGCGCACCGCGTGAGCTGCTGCTGCGAACCAAGGCGAGCCTGCGTCATG
CGGCGAGCGCTTCGCATGCGGCCGCTTTCGAACGGGAAACGGCCGAACAGAAGTGGTCAATGCAGCAACCCGAGTTTCTC
TCGATGCTGGGGAGCGCGCAAAAATGA

Upstream 100 bases:

>100_bases
AACGCGTTGAGATCTTCACGTCATTTCCGCTTTCGAATTTCGGTAAGGTCTCGAAGAAAGATCTGGCCGCAACGGTGGCC
GAGCGCGTACAGGCGGCGCA

Downstream 100 bases:

>100_bases
GCGCCATTCTCTCACCGCTTCTCTACGAAAAGCGCGCGCGCGTCGCCACCTTGACGATCAACCGGCCAGATGTGATGAAC
GCGATGGACTCGCACGCGCA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKCYVTAIHENGIATLTLADPERRNVLSEVLCEQLIDAVAAAHADQDVRALVIAAQGPAFCAGAHRDDLRAAAEGDARVI
GKVYQTFMDVANSPLPTIAAINGPAVGAGMNLALACDLRIASSSARFDTRFIGIGLHPGGGHGWMLVRAVGWQNAASLLL
LGAAVNAAEAMRMGLVSACVADDGLADAVASACRRIRSAPRELLLRTKASLRHAASASHAAAFERETAEQKWSMQQPEFL
SMLGSAQK

Sequences:

>Translated_248_residues
MKCYVTAIHENGIATLTLADPERRNVLSEVLCEQLIDAVAAAHADQDVRALVIAAQGPAFCAGAHRDDLRAAAEGDARVI
GKVYQTFMDVANSPLPTIAAINGPAVGAGMNLALACDLRIASSSARFDTRFIGIGLHPGGGHGWMLVRAVGWQNAASLLL
LGAAVNAAEAMRMGLVSACVADDGLADAVASACRRIRSAPRELLLRTKASLRHAASASHAAAFERETAEQKWSMQQPEFL
SMLGSAQK
>Mature_248_residues
MKCYVTAIHENGIATLTLADPERRNVLSEVLCEQLIDAVAAAHADQDVRALVIAAQGPAFCAGAHRDDLRAAAEGDARVI
GKVYQTFMDVANSPLPTIAAINGPAVGAGMNLALACDLRIASSSARFDTRFIGIGLHPGGGHGWMLVRAVGWQNAASLLL
LGAAVNAAEAMRMGLVSACVADDGLADAVASACRRIRSAPRELLLRTKASLRHAASASHAAAFERETAEQKWSMQQPEFL
SMLGSAQK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=206, Percent_Identity=31.5533980582524, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI4502327, Length=212, Percent_Identity=36.3207547169811, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI70995211, Length=246, Percent_Identity=27.6422764227642, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI194097323, Length=206, Percent_Identity=29.6116504854369, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI68989263, Length=198, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI260274832, Length=212, Percent_Identity=24.5283018867925, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=212, Percent_Identity=24.5283018867925, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI260275230, Length=212, Percent_Identity=24.5283018867925, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4757968, Length=226, Percent_Identity=26.1061946902655, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI49355787, Length=226, Percent_Identity=26.1061946902655, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4757966, Length=226, Percent_Identity=25.6637168141593, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI51538581, Length=226, Percent_Identity=25.6637168141593, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI221136756, Length=210, Percent_Identity=28.0952380952381, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221136753, Length=210, Percent_Identity=28.0952380952381, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI25453481, Length=226, Percent_Identity=25.6637168141593, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI51538573, Length=226, Percent_Identity=25.6637168141593, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221307494, Length=210, Percent_Identity=28.0952380952381, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1787659, Length=217, Percent_Identity=34.5622119815668, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787660, Length=236, Percent_Identity=31.7796610169492, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788597, Length=215, Percent_Identity=28.3720930232558, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=27.3684210526316, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788682, Length=213, Percent_Identity=26.7605633802817, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=194, Percent_Identity=29.8969072164948, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=220, Percent_Identity=30, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=182, Percent_Identity=34.0659340659341, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=25.6198347107438, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=224, Percent_Identity=28.125, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=218, Percent_Identity=33.4862385321101, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=254, Percent_Identity=26.7716535433071, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=192, Percent_Identity=26.5625, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=170, Percent_Identity=30.5882352941176, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=178, Percent_Identity=33.1460674157303, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653477, Length=178, Percent_Identity=33.1460674157303, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24654903, Length=226, Percent_Identity=25.2212389380531, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922422, Length=218, Percent_Identity=27.5229357798165, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24653139, Length=180, Percent_Identity=28.8888888888889, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24650670, Length=176, Percent_Identity=32.3863636363636, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19920382, Length=210, Percent_Identity=30, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 25996; Mature: 25996

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCYVTAIHENGIATLTLADPERRNVLSEVLCEQLIDAVAAAHADQDVRALVIAAQGPAF
CEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCE
CAGAHRDDLRAAAEGDARVIGKVYQTFMDVANSPLPTIAAINGPAVGAGMNLALACDLRI
ECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEEEE
ASSSARFDTRFIGIGLHPGGGHGWMLVRAVGWQNAASLLLLGAAVNAAEAMRMGLVSACV
CCCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ADDGLADAVASACRRIRSAPRELLLRTKASLRHAASASHAAAFERETAEQKWSMQQPEFL
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
SMLGSAQK
HHHCCCCC
>Mature Secondary Structure
MKCYVTAIHENGIATLTLADPERRNVLSEVLCEQLIDAVAAAHADQDVRALVIAAQGPAF
CEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCE
CAGAHRDDLRAAAEGDARVIGKVYQTFMDVANSPLPTIAAINGPAVGAGMNLALACDLRI
ECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEEEE
ASSSARFDTRFIGIGLHPGGGHGWMLVRAVGWQNAASLLLLGAAVNAAEAMRMGLVSACV
CCCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ADDGLADAVASACRRIRSAPRELLLRTKASLRHAASASHAAAFERETAEQKWSMQQPEFL
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
SMLGSAQK
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]