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The map label for this gene is mhpA [H]

Identifier: 116688185

GI number: 116688185

Start: 178301

End: 179893

Strand: Direct

Name: mhpA [H]

Synonym: Bcen2424_0161

Alternate gene names: 116688185

Gene position: 178301-179893 (Clockwise)

Preceding gene: 116688184

Following gene: 116688186

Centisome position: 5.12

GC content: 65.73

Gene sequence:

>1593_bases
ATGACGATGGAACGCAACGAACATTTCGACGTCGTCGTGGTGGGCTTCGGCCCGTCCGGCGCAGTCGCGGCGGGTCTGCT
GGGTCAGCGCGGCCGCAAGGTGCTGTGCGTGGACCGCGCGCGCGAGGTCTACGACAAGCCGCGCGCGATCGCCGTCGATC
ACGAGATCATGCGCGTGTTCCAGGGGCTCGGCGTGGTCGAGCGGATTGCGCAGCACGTGGCCGTGTTTCCGCCGTCCGAG
TATTACGGCGTGGACGGTCAGCTGATCAAGCGTATCGATACGGTGCCCAAACCCTGGCCGCTCGGCTACATGCCGACGAT
GGTGTTCACGCAACCGCCGGTCGAAGCCGCGCTGCGCGAACATGCGAGCGCGTATGAGTCGGTTGAGGTTTCGCTCGGCA
CCGCGCTCGTGGGTTTCGAAGAGACCGCTGACGGCGTCACCGTTCAACTGAAGGACGACACCGAAGGCGCGGTCACCCGC
ACCGTGACCTGTGATTACCTGATCGGCTGCGACGGCGCGTCGAGCACGGTGCGCACGCAGCTAAGCATCGAGTACGAAGA
CCTCGTATTCGACGAGCCCTGGCTCGTGGTCGACTTGCGCGTGAACGAACGCGGCATCGAGAAACTGCCGAAGGTCGCGA
TCCAGTACTGCGAGCCTTCACGTCCGTGCACGTACATCGTTGGGCCGGGCGGTCATCGCCGCTGGGAAATCATGCTGATG
CCCGGCGAAGATCCCCGCGAAATGCAGACTGAAGAGCAGGTGTGGAAGCTGCTGTCGCGCTGGCTCACGCGTGAAGACGC
CGAACTGTGGCGGGCGGCGAGCTATCGCTTCCACGCGCTCGTCGCGAAGGAGTGGCGGCGAGGGCGCGTTTTCATCGCAG
GCGATGCGGCGCACCAGCAGCCGCCGTTCATCGGCCAGGGGATGTGCCAAGGTATCCGCGACGTGGTCAACCTCGTGTGG
AAGCTCGACGCGGTGATGGACGGCAGCGCGCAGCCCGAACTGCTCGATACGTATGGCGCCGAACGCAGCCTGCATGTGCG
TCAGTTGACCACGCGCATCAAGGAGATCGGCAAGCTGATCTGCGAACGCGATGTGACCGCCGCGCGCGAGCGCGATGCGC
GGCTCTTGCGCGAGGCGGGCGGTCAGGTGAAGACGATCGCGCGTCAGGACATCGTGCCGAAGCTCGATGCAGGTTTGCTT
TCGCCGATCCCACATGCAAGCAACGGCAGCCTGTTCCCGCAACCGCGCGTGCGCCACGCTGACGATTCCGCGCTGCTCGA
TGCGGTGGCCGGCGGTAATTGGCGCGTGGTGACGCTAGGCGGCGCGATGGTGTCGGAGCAGGTCGCGAGCGCGGCGGCGT
CGATCGGTGCGCAGGTGATCGAATTGGCACCGGACGGTAACGCACATCGCGCGGCGGTGCTGCCCGACACGCGTAGCATC
GTCGAGGTGGACGGCGTGCTTGCCGCGTGGTTCGAGCGCAATCAGTGCGGTGCGGCGATCGTGCGTCCCGATCACTACGT
GTACGGCGTCGCGAGCAGTGCGCAGAGTCTGGCCGAAGCACTCGCGCAACTCGATACCGCACTGCACGGTTAG

Upstream 100 bases:

>100_bases
CGCGCGAGCCGTCGGTGTGGCTCAAGGCGGGCGACCGCGTCGAAGTGGACATCGAACGTATCGGCCGTCTGCAAAACACG
GTGCAGGACGAAGCGGGCGC

Downstream 100 bases:

>100_bases
CCGAGCCAAGGGGAGGGGCAGGCTGCGGGATCCCCGCCTGTCCATAGCACCGCAGTACGGCAACGCCGCAACATCGAAGC
AAAACGCACCGCGCCGCATC

Product: monooxygenase, FAD-binding

Products: tetrachlorohydroquinone; NADP+; chloride

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTMERNEHFDVVVVGFGPSGAVAAGLLGQRGRKVLCVDRAREVYDKPRAIAVDHEIMRVFQGLGVVERIAQHVAVFPPSE
YYGVDGQLIKRIDTVPKPWPLGYMPTMVFTQPPVEAALREHASAYESVEVSLGTALVGFEETADGVTVQLKDDTEGAVTR
TVTCDYLIGCDGASSTVRTQLSIEYEDLVFDEPWLVVDLRVNERGIEKLPKVAIQYCEPSRPCTYIVGPGGHRRWEIMLM
PGEDPREMQTEEQVWKLLSRWLTREDAELWRAASYRFHALVAKEWRRGRVFIAGDAAHQQPPFIGQGMCQGIRDVVNLVW
KLDAVMDGSAQPELLDTYGAERSLHVRQLTTRIKEIGKLICERDVTAARERDARLLREAGGQVKTIARQDIVPKLDAGLL
SPIPHASNGSLFPQPRVRHADDSALLDAVAGGNWRVVTLGGAMVSEQVASAAASIGAQVIELAPDGNAHRAAVLPDTRSI
VEVDGVLAAWFERNQCGAAIVRPDHYVYGVASSAQSLAEALAQLDTALHG

Sequences:

>Translated_530_residues
MTMERNEHFDVVVVGFGPSGAVAAGLLGQRGRKVLCVDRAREVYDKPRAIAVDHEIMRVFQGLGVVERIAQHVAVFPPSE
YYGVDGQLIKRIDTVPKPWPLGYMPTMVFTQPPVEAALREHASAYESVEVSLGTALVGFEETADGVTVQLKDDTEGAVTR
TVTCDYLIGCDGASSTVRTQLSIEYEDLVFDEPWLVVDLRVNERGIEKLPKVAIQYCEPSRPCTYIVGPGGHRRWEIMLM
PGEDPREMQTEEQVWKLLSRWLTREDAELWRAASYRFHALVAKEWRRGRVFIAGDAAHQQPPFIGQGMCQGIRDVVNLVW
KLDAVMDGSAQPELLDTYGAERSLHVRQLTTRIKEIGKLICERDVTAARERDARLLREAGGQVKTIARQDIVPKLDAGLL
SPIPHASNGSLFPQPRVRHADDSALLDAVAGGNWRVVTLGGAMVSEQVASAAASIGAQVIELAPDGNAHRAAVLPDTRSI
VEVDGVLAAWFERNQCGAAIVRPDHYVYGVASSAQSLAEALAQLDTALHG
>Mature_529_residues
TMERNEHFDVVVVGFGPSGAVAAGLLGQRGRKVLCVDRAREVYDKPRAIAVDHEIMRVFQGLGVVERIAQHVAVFPPSEY
YGVDGQLIKRIDTVPKPWPLGYMPTMVFTQPPVEAALREHASAYESVEVSLGTALVGFEETADGVTVQLKDDTEGAVTRT
VTCDYLIGCDGASSTVRTQLSIEYEDLVFDEPWLVVDLRVNERGIEKLPKVAIQYCEPSRPCTYIVGPGGHRRWEIMLMP
GEDPREMQTEEQVWKLLSRWLTREDAELWRAASYRFHALVAKEWRRGRVFIAGDAAHQQPPFIGQGMCQGIRDVVNLVWK
LDAVMDGSAQPELLDTYGAERSLHVRQLTTRIKEIGKLICERDVTAARERDARLLREAGGQVKTIARQDIVPKLDAGLLS
PIPHASNGSLFPQPRVRHADDSALLDAVAGGNWRVVTLGGAMVSEQVASAAASIGAQVIELAPDGNAHRAAVLPDTRSIV
EVDGVLAAWFERNQCGAAIVRPDHYVYGVASSAQSLAEALAQLDTALHG

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=537, Percent_Identity=30.7262569832402, Blast_Score=217, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.50

Molecular weight: Translated: 58150; Mature: 58019

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMERNEHFDVVVVGFGPSGAVAAGLLGQRGRKVLCVDRAREVYDKPRAIAVDHEIMRVF
CCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHCCCCEEEECHHHHHHH
QGLGVVERIAQHVAVFPPSEYYGVDGQLIKRIDTVPKPWPLGYMPTMVFTQPPVEAALRE
HHCCHHHHHHHHEEECCCCHHCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHH
HASAYESVEVSLGTALVGFEETADGVTVQLKDDTEGAVTRTVTCDYLIGCDGASSTVRTQ
HHHHHHHEEEECCEEEEECCCCCCCEEEEEECCCCCCEEEEEEEEEEEECCCCCCCEEEE
LSIEYEDLVFDEPWLVVDLRVNERGIEKLPKVAIQYCEPSRPCTYIVGPGGHRRWEIMLM
EEEEHHHEEECCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEE
PGEDPREMQTEEQVWKLLSRWLTREDAELWRAASYRFHALVAKEWRRGRVFIAGDAAHQQ
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCC
PPFIGQGMCQGIRDVVNLVWKLDAVMDGSAQPELLDTYGAERSLHVRQLTTRIKEIGKLI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
CERDVTAARERDARLLREAGGQVKTIARQDIVPKLDAGLLSPIPHASNGSLFPQPRVRHA
HHCCHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DDSALLDAVAGGNWRVVTLGGAMVSEQVASAAASIGAQVIELAPDGNAHRAAVLPDTRSI
CCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCHH
VEVDGVLAAWFERNQCGAAIVRPDHYVYGVASSAQSLAEALAQLDTALHG
HHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMERNEHFDVVVVGFGPSGAVAAGLLGQRGRKVLCVDRAREVYDKPRAIAVDHEIMRVF
CCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHCCCCEEEECHHHHHHH
QGLGVVERIAQHVAVFPPSEYYGVDGQLIKRIDTVPKPWPLGYMPTMVFTQPPVEAALRE
HHCCHHHHHHHHEEECCCCHHCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHH
HASAYESVEVSLGTALVGFEETADGVTVQLKDDTEGAVTRTVTCDYLIGCDGASSTVRTQ
HHHHHHHEEEECCEEEEECCCCCCCEEEEEECCCCCCEEEEEEEEEEEECCCCCCCEEEE
LSIEYEDLVFDEPWLVVDLRVNERGIEKLPKVAIQYCEPSRPCTYIVGPGGHRRWEIMLM
EEEEHHHEEECCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEE
PGEDPREMQTEEQVWKLLSRWLTREDAELWRAASYRFHALVAKEWRRGRVFIAGDAAHQQ
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCC
PPFIGQGMCQGIRDVVNLVWKLDAVMDGSAQPELLDTYGAERSLHVRQLTTRIKEIGKLI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
CERDVTAARERDARLLREAGGQVKTIARQDIVPKLDAGLLSPIPHASNGSLFPQPRVRHA
HHCCHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DDSALLDAVAGGNWRVVTLGGAMVSEQVASAAASIGAQVIELAPDGNAHRAAVLPDTRSI
CCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCHH
VEVDGVLAAWFERNQCGAAIVRPDHYVYGVASSAQSLAEALAQLDTALHG
HHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pentachlorophenol; NADPH; H+; O2

Specific reaction: pentachlorophenol + NADPH + H+ + O2 = tetrachlorohydroquinone + NADP+ + chloride

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA