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The map label for this gene is rep [H]

Identifier: 116688174

GI number: 116688174

Start: 164978

End: 167065

Strand: Direct

Name: rep [H]

Synonym: Bcen2424_0150

Alternate gene names: 116688174

Gene position: 164978-167065 (Clockwise)

Preceding gene: 116688165

Following gene: 116688176

Centisome position: 4.74

GC content: 66.09

Gene sequence:

>2088_bases
ATGTCCGCAGGCCTCAATCCCGCTCAGAACGAAGCGGTGCGCTATCTCGACGGTCCCTGTCTCGTGCTCGCCGGCGCGGG
CAGCGGCAAGACACGCGTGATCACGCAGAAGATCGCGCATCTGATCGAAGCGAAGGGCTTCGAGCCGCGCCATATCGCCG
CGGTCACGTTCACGAACAAGGCGGCCGCCGAAATGCGCGAGCGCGTGTCGAAGCTGCTGGAAGGCAAGACCCTCACCACG
CCGGGCAAGGAAGGCCGCAAGGTGCCCGTCAACCAGCTCACCGTCTGCACGTTCCACTCGCTGGGCGTGCAGATCCTGCG
CCAGGAGGCCGAGCACGTCGGCCTGAAGCCGCAGTTCTCGATCATGGATTCGGACGACTGCTTCGGGATGATCCAGGAGC
AGCTCGGCACCACCGACAAAGGGCTGATCCGCAAGATCCAGAGCATCATCTCGCTGTGGAAGAACGGCCTGATCATGCCC
GACGAAGCGATGGCGATCGCGGCCAACGAGGACGAGCACCAGGCCGCGCTGGTCTACCGCAACTACGTGGCGACGCTGCA
CGCGTACCAGGCGGTCGACTTCGACGACCTGATCCGCCTGCCGGCCGAGCTGTTCGCGAAGAACGAGCAGGTGCGCGACC
GCTGGCAGAACAAGCTGCGCTACCTGCTGATCGACGAGTACCAGGACACCAATGCGTGCCAGTACGAGCTGCTGAAGCAG
CTCGCGGGCCCGCGCGCCGCGTTCACGGCGGTCGGCGACGACGACCAGGCGATCTACGGCTGGCGCGGCGCGACGCTGGA
GAACCTCGCGCAGCTTGGCAAGGATTTTCCGAAGCTGCACGTGATCAAGCTCGAGCAGAACTACCGGTCGACGGTGCGGA
TACTGACCGCCGCGAACAACGTGATCGCGAACAACCCGAAGCTGTTCGAGAAGAAGCTGTGGTCCGAGCACGGGATGGGC
GATTCGATCACCGTCACGGCGTGTAACGACGAGGAGCACGAGGCCGAATCGGTCGTGTTCCGGCTGTCCGCGCACAAGTT
CGAGCGGCGCGCGCAGTTCCGCGACTACGCGATCCTGTACCGCGGCAATTTCCAGGCGCGCATCTTCGAGCAGGTGCTGC
GGCGCGAGCGGATTCCGTACGTGCTGTCGGGCGGCCAGTCGTTCTTCGACAAGGCCGAGATCAAGGATCTGTGCGCGTAC
CTGCGGCTGATCGCGAACGCCGACGACGATCCCGCGTTCATCCGCGCGGTCACGACGCCGCGCCGCGGCATCGGCAACAC
GACGCTGGAAGCGCTCGGCTCGTTCGCGGGGCAGGCGAAGGTGTCGCTGTTCGAGGCCGTCTACATGGGCGGGATCGAGG
CGCGGCTGTCGGCGCGCCAGGTCGAGCCGCTGCGGATGTTCTGCGACTTCATCCAGCGCCTGACCGAGCGCGCGGACAAG
GAGCCCGCGACCGTCGTGCTCGACGACATGATGGAGGCGATCCACTACGAGGCGTACCTGTACGACGCGTTCGACGAGCG
GCAGGCGCAGTCGAAGTGGCAGAACGTGCTCGAATTCCTCGAATGGCTGAAGCGCAAGGGCACGAAGCCCGAGAGGGACG
CCGTCGACGGCGAGGCCGAAGGTTTCCACAACGCGGACGGCCTCGCCGATACGGGCAAGAACCTGCTCGGCCTGATCCAG
ACCGTCGCGCTGATGTCGATGCTCGAGGGCAAGGACGAGGACCCGGACGCCGTGCGGCTGTCGACCGTCCATGCGTCGAA
GGGGCTCGAGTATCCGCACGTGTTCCTGGTCGGCGTCGAGGAAGGCATCATGCCGCACCGCGGCGGCAGCGAGGACGACG
GCCCGATCGACAACGAGCGGATCGAGGAGGAGCGCCGGCTGATGTACGTCGCGATCACGCGCGCGCAGCGCAGCCTGCAC
CTGAACTGGTGCAAGAAGCGCAAGCGGGCGCGCGAGACGGTCGTGTGCGAACCGTCGCGCTTCATCCCGGAGATGGGGCT
CGACGAAGCGCCGCCGCCGACGCCGGAAGAAGCGCCGATGTCGCCGAAGGACCGGCTCGCGAGCCTGAAGGCATTGCTGC
AGAAGTGA

Upstream 100 bases:

>100_bases
AACATGCGCGTCCTGCCCCGCGTGTCCGCCTGCTTCGCCAGCCTGCCGCGCACGGCATCCTCTCGGCGTCCGCCGTCAAC
CAGCAACACCATCCGCCATC

Downstream 100 bases:

>100_bases
CGCGGCGGCAGTGCCGCGCACGCTGGCGCGGCCAACCGGCGACACAAAAACGAAACCCCCGCGATGCAAGGGCGTCGCGG
GGGTTTTTTACGGATGGCCG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAAAEMRERVSKLLEGKTLTT
PGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQLGTTDKGLIRKIQSIISLWKNGLIMP
DEAMAIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELLKQ
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMG
DSITVTACNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAY
LRLIANADDDPAFIRAVTTPRRGIGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSARQVEPLRMFCDFIQRLTERADK
EPATVVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPERDAVDGEAEGFHNADGLADTGKNLLGLIQ
TVALMSMLEGKDEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGSEDDGPIDNERIEEERRLMYVAITRAQRSLH
LNWCKKRKRARETVVCEPSRFIPEMGLDEAPPPTPEEAPMSPKDRLASLKALLQK

Sequences:

>Translated_695_residues
MSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAAAEMRERVSKLLEGKTLTT
PGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQLGTTDKGLIRKIQSIISLWKNGLIMP
DEAMAIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELLKQ
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMG
DSITVTACNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAY
LRLIANADDDPAFIRAVTTPRRGIGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSARQVEPLRMFCDFIQRLTERADK
EPATVVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPERDAVDGEAEGFHNADGLADTGKNLLGLIQ
TVALMSMLEGKDEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGSEDDGPIDNERIEEERRLMYVAITRAQRSLH
LNWCKKRKRARETVVCEPSRFIPEMGLDEAPPPTPEEAPMSPKDRLASLKALLQK
>Mature_694_residues
SAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAAAEMRERVSKLLEGKTLTTP
GKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQLGTTDKGLIRKIQSIISLWKNGLIMPD
EAMAIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELLKQL
AGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMGD
SITVTACNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAYL
RLIANADDDPAFIRAVTTPRRGIGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSARQVEPLRMFCDFIQRLTERADKE
PATVVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPERDAVDGEAEGFHNADGLADTGKNLLGLIQT
VALMSMLEGKDEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGSEDDGPIDNERIEEERRLMYVAITRAQRSLHL
NWCKKRKRARETVVCEPSRFIPEMGLDEAPPPTPEEAPMSPKDRLASLKALLQK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=701, Percent_Identity=47.2182596291013, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI2367296, Length=665, Percent_Identity=35.187969924812, Blast_Score=355, Evalue=7e-99,
Organism=Escherichia coli, GI1787196, Length=379, Percent_Identity=27.4406332453826, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=730, Percent_Identity=28.2191780821918, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6324477, Length=660, Percent_Identity=24.2424242424242, Blast_Score=114, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78196; Mature: 78065

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNK
CCCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCH
AAAEMRERVSKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFS
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGMIQEQLGTTDKGLIRKIQSIISLWKNGLIMPDEAMAIAANEDEHQAALVYR
ECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
NYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELLKQ
HHHHHHHHHHCCCHHHHHHCCHHHHHCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRILTAANN
HCCCCEEEEEECCCCCEEEECCCCCHHHHHHHCCCCCCEEEEEECCCCHHHEEHHHHCCC
VIANNPKLFEKKLWSEHGMGDSITVTACNDEEHEAESVVFRLSAHKFERRAQFRDYAILY
HHCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
RGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAFIRAVTTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
RRGIGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSARQVEPLRMFCDFIQRLTERADK
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EPATVVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPERDAVDGEAE
CCCEEHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GFHNADGLADTGKNLLGLIQTVALMSMLEGKDEDPDAVRLSTVHASKGLEYPHVFLVGVE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEEC
EGIMPHRGGSEDDGPIDNERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSR
CCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHCCCEEECCHH
FIPEMGLDEAPPPTPEEAPMSPKDRLASLKALLQK
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNK
CCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCH
AAAEMRERVSKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFS
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGMIQEQLGTTDKGLIRKIQSIISLWKNGLIMPDEAMAIAANEDEHQAALVYR
ECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
NYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELLKQ
HHHHHHHHHHCCCHHHHHHCCHHHHHCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHVIKLEQNYRSTVRILTAANN
HCCCCEEEEEECCCCCEEEECCCCCHHHHHHHCCCCCCEEEEEECCCCHHHEEHHHHCCC
VIANNPKLFEKKLWSEHGMGDSITVTACNDEEHEAESVVFRLSAHKFERRAQFRDYAILY
HHCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
RGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAFIRAVTTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
RRGIGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSARQVEPLRMFCDFIQRLTERADK
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EPATVVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPERDAVDGEAE
CCCEEHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GFHNADGLADTGKNLLGLIQTVALMSMLEGKDEDPDAVRLSTVHASKGLEYPHVFLVGVE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEEC
EGIMPHRGGSEDDGPIDNERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSR
CCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHCCCEEECCHH
FIPEMGLDEAPPPTPEEAPMSPKDRLASLKALLQK
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]