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The map label for this gene is yafC [H]

Identifier: 116688155

GI number: 116688155

Start: 142442

End: 143326

Strand: Reverse

Name: yafC [H]

Synonym: Bcen2424_0131

Alternate gene names: 116688155

Gene position: 143326-142442 (Counterclockwise)

Preceding gene: 116688157

Following gene: 116688150

Centisome position: 4.11

GC content: 69.83

Gene sequence:

>885_bases
ATGGAAAACCTCACCGGCATCGTCGCGTTCGTCCGCACCGCCGAAGCGCTGAGCTTCGTCGCGGCCGGCCGCGCGCTCGG
CATCTCGGCGTCGGCGGTCGGCAAGACGATCGCGAAACTCGAGCAGTCGCTCGGCGTGCGGCTGTTCAACCGCACGACGC
GCCGCGTGACGCTCACCGACGAGGGGCGGCATTTCTACGAGCGCTGCCAGCGCATCCTCGAGGATTTCCGCGACGCGGAG
GCGACCGTGACGGCGTCCGCGCAGCGCCCGCGCGGCAAGCTGCGCGTGAGCCTGCCGGTGATCGGCTACCGCTTCCTGCT
GCCGGTGCTGCCCGAATTCCGGCAGCGCTATCCGGACGTCGAGCTCGATCTCGATTTCAACGACCGGATGGTCGACGTCG
TCGAAGGCGGCTTCGACGCGGTGATCCGCAGCGGCCCGCTGTCCGATTCGAGCCTGATGTCGCGGCGGCTCGGCCCGTTC
GCGTTCGTGCTGTGCGCGACGCCCGCCTATCTCGCGCAGGCCGGCACGCCGCGCACGCCGCGCGAGCTCGAAGCGCACGA
CTGCGTGCGCTACTGCTTCCCGACCACCGGCAAGCTGCAGGACTGGGCGCTCGCGGCCGACGACGGCACGCCGCTCACGC
TGCGCACCGCGATGACCTGCAACAACATGGAAGCGCTGCGTGGCGCCGTGCTCGCGGGGCTCGGCATCGGCTACATGCCC
GACTTCCTCGCGCGCGACGCGCTCGAATACGGCACGCTCGTCACCGTGCTCGACGACTACCGGATCGCGCCGGGGCAGTT
CTCGATCGTGTGGCCGTCGAGCCGGCAGTTGTCGCCGAAGCTGCGCGTGTTCGTCGATTTCCTGTGCGAGCGGCTGTTCA
GGTAG

Upstream 100 bases:

>100_bases
ATCCGCGAAAAACCACGCGAAGCTCCGCATATAACGGACTAAAATTCCGCAATACACCTGCCTGCGTGCGTCACCGCACG
TCCTTCGATTCGATGGAAAG

Downstream 100 bases:

>100_bases
TCGCGCCGATCCGCTCCGCTTCCGCCGCCGGCAGCAGCGCGTCGCTGTCGTCCTTCAGCCCGACGCCCGTCAACTGGAGC
CTGCGCGCATGCGTCATCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MENLTGIVAFVRTAEALSFVAAGRALGISASAVGKTIAKLEQSLGVRLFNRTTRRVTLTDEGRHFYERCQRILEDFRDAE
ATVTASAQRPRGKLRVSLPVIGYRFLLPVLPEFRQRYPDVELDLDFNDRMVDVVEGGFDAVIRSGPLSDSSLMSRRLGPF
AFVLCATPAYLAQAGTPRTPRELEAHDCVRYCFPTTGKLQDWALAADDGTPLTLRTAMTCNNMEALRGAVLAGLGIGYMP
DFLARDALEYGTLVTVLDDYRIAPGQFSIVWPSSRQLSPKLRVFVDFLCERLFR

Sequences:

>Translated_294_residues
MENLTGIVAFVRTAEALSFVAAGRALGISASAVGKTIAKLEQSLGVRLFNRTTRRVTLTDEGRHFYERCQRILEDFRDAE
ATVTASAQRPRGKLRVSLPVIGYRFLLPVLPEFRQRYPDVELDLDFNDRMVDVVEGGFDAVIRSGPLSDSSLMSRRLGPF
AFVLCATPAYLAQAGTPRTPRELEAHDCVRYCFPTTGKLQDWALAADDGTPLTLRTAMTCNNMEALRGAVLAGLGIGYMP
DFLARDALEYGTLVTVLDDYRIAPGQFSIVWPSSRQLSPKLRVFVDFLCERLFR
>Mature_294_residues
MENLTGIVAFVRTAEALSFVAAGRALGISASAVGKTIAKLEQSLGVRLFNRTTRRVTLTDEGRHFYERCQRILEDFRDAE
ATVTASAQRPRGKLRVSLPVIGYRFLLPVLPEFRQRYPDVELDLDFNDRMVDVVEGGFDAVIRSGPLSDSSLMSRRLGPF
AFVLCATPAYLAQAGTPRTPRELEAHDCVRYCFPTTGKLQDWALAADDGTPLTLRTAMTCNNMEALRGAVLAGLGIGYMP
DFLARDALEYGTLVTVLDDYRIAPGQFSIVWPSSRQLSPKLRVFVDFLCERLFR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=35.8885017421603, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=36.0824742268041, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=34.0277777777778, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=31.740614334471, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI87081978, Length=288, Percent_Identity=34.0277777777778, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=27.1477663230241, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1789440, Length=289, Percent_Identity=29.4117647058824, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=26.1904761904762, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787879, Length=284, Percent_Identity=28.169014084507, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=28.7671232876712, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1786508, Length=270, Percent_Identity=24.4444444444444, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788481, Length=286, Percent_Identity=27.6223776223776, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787601, Length=162, Percent_Identity=31.4814814814815, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788748, Length=178, Percent_Identity=34.2696629213483, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788706, Length=301, Percent_Identity=24.9169435215947, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082132, Length=271, Percent_Identity=28.0442804428044, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI145693105, Length=258, Percent_Identity=27.1317829457364, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI157672245, Length=117, Percent_Identity=35.042735042735, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790208, Length=258, Percent_Identity=27.1317829457364, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32665; Mature: 32665

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLTGIVAFVRTAEALSFVAAGRALGISASAVGKTIAKLEQSLGVRLFNRTTRRVTLTD
CCCHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHCCEEECCCCEEEEEEC
EGRHFYERCQRILEDFRDAEATVTASAQRPRGKLRVSLPVIGYRFLLPVLPEFRQRYPDV
CCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCE
ELDLDFNDRMVDVVEGGFDAVIRSGPLSDSSLMSRRLGPFAFVLCATPAYLAQAGTPRTP
EEEECCCCCCHHHHHCCHHHHHHCCCCCHHHHHHHCCCHHEEHHHCCHHHHHHCCCCCCC
RELEAHDCVRYCFPTTGKLQDWALAADDGTPLTLRTAMTCNNMEALRGAVLAGLGIGYMP
CCCCHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCH
DFLARDALEYGTLVTVLDDYRIAPGQFSIVWPSSRQLSPKLRVFVDFLCERLFR
HHHHHHHHHHCCEEEEECCCEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENLTGIVAFVRTAEALSFVAAGRALGISASAVGKTIAKLEQSLGVRLFNRTTRRVTLTD
CCCHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHCCEEECCCCEEEEEEC
EGRHFYERCQRILEDFRDAEATVTASAQRPRGKLRVSLPVIGYRFLLPVLPEFRQRYPDV
CCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCE
ELDLDFNDRMVDVVEGGFDAVIRSGPLSDSSLMSRRLGPFAFVLCATPAYLAQAGTPRTP
EEEECCCCCCHHHHHCCHHHHHHCCCCCHHHHHHHCCCHHEEHHHCCHHHHHHCCCCCCC
RELEAHDCVRYCFPTTGKLQDWALAADDGTPLTLRTAMTCNNMEALRGAVLAGLGIGYMP
CCCCHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCH
DFLARDALEYGTLVTVLDDYRIAPGQFSIVWPSSRQLSPKLRVFVDFLCERLFR
HHHHHHHHHHCCEEEEECCCEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]