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The map label for this gene is rob [H]

Identifier: 116688150

GI number: 116688150

Start: 135893

End: 136774

Strand: Reverse

Name: rob [H]

Synonym: Bcen2424_0126

Alternate gene names: 116688150

Gene position: 136774-135893 (Counterclockwise)

Preceding gene: 116688155

Following gene: 116688147

Centisome position: 3.93

GC content: 69.5

Gene sequence:

>882_bases
ATGACTCCAGCCGAAACGAAAACCGACCCGCCCGCGTGGGTGCGCTACGCGGCAAGCGTGGCGCGGGTCCACGCGTACAT
CAGCGCGCATCTCGACGGCCCGCTCGACCTGAACCGGCTCGCCGAAGTCGCGTGCCTGTCGCCGTATCACTGGCAGCGCG
TCTACCGCGCGCTGTACGGCGAGTCGATCGTGCTGACCGTGCGGCGGATGCGGATCGTGCGGGCGTCCGAGGATCTGGTC
TGCACCGCGCGGCCGCTCGATGAAATCGCGCGGCGCGCGGGCTACGGGTCGACGCACGCATTCGCGCGGGCGTTTCGCGA
CGCGTACGGCGTGCTGCCCGCGCAGCACCGGCGCACCGGCACCCACCGGCCGATTTATCCACTACAGCGAGGAGAGGCAG
ACATGTTCAAGCACGAGGTCGAGATCCGGCGCCTGCCGGAACTGCGCGGCTACGCGGTAACGCACACCGGTGCGTACATG
AAGGTGGGGGACGCGTTCGGGCGGATCGGCGCGTGGGTGGGGCAGCACGGGCTGATCGGGCCGGGCGCGAAGATGATCGG
GATTTTCTACGACGATCCGGACACGACGCCCGAAGCGCAGTTGCGCGCGAAAGCGTGCTTCATGCCGGCCGACGGCGCGC
CGGACGTCGCACCCGTGCCGCCGGTCGAGCGGGTGACGATCGCCGGCGGCGAATACGCGGTGCTGCTGCATACGGGGCCG
TATGCGGACCTGAAAACGGCCTACCAGTGGCTGTACGGCGACTGGCTGCGCCACTCGGGCCGCGAAGCCGCCGACGCGCC
GCCGTTCGAGCTATACCTCAATACGCCGATGGATACGGCGCCGGCCGACCTGCGCACCGAAATTCACCTGCCGCTGCGCT
GA

Upstream 100 bases:

>100_bases
GAACTCAAAAAGGGCCGCATCGACGGGTGTCCGGCATTTCCGCAAGATTTGTCCATTCTGCCCGTGCTACCGTGGCGTCG
GTTCCAGCCCGGACACGCCG

Downstream 100 bases:

>100_bases
GCGACGGGTGTGCCGTGGCGCCGTGTATCTGGACGGACCGCGACGATATTGCTGGTGCGAGGATGCACGAGACGACATCG
GGCATGCCGGTGCGCCGGCC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTPAETKTDPPAWVRYAASVARVHAYISAHLDGPLDLNRLAEVACLSPYHWQRVYRALYGESIVLTVRRMRIVRASEDLV
CTARPLDEIARRAGYGSTHAFARAFRDAYGVLPAQHRRTGTHRPIYPLQRGEADMFKHEVEIRRLPELRGYAVTHTGAYM
KVGDAFGRIGAWVGQHGLIGPGAKMIGIFYDDPDTTPEAQLRAKACFMPADGAPDVAPVPPVERVTIAGGEYAVLLHTGP
YADLKTAYQWLYGDWLRHSGREAADAPPFELYLNTPMDTAPADLRTEIHLPLR

Sequences:

>Translated_293_residues
MTPAETKTDPPAWVRYAASVARVHAYISAHLDGPLDLNRLAEVACLSPYHWQRVYRALYGESIVLTVRRMRIVRASEDLV
CTARPLDEIARRAGYGSTHAFARAFRDAYGVLPAQHRRTGTHRPIYPLQRGEADMFKHEVEIRRLPELRGYAVTHTGAYM
KVGDAFGRIGAWVGQHGLIGPGAKMIGIFYDDPDTTPEAQLRAKACFMPADGAPDVAPVPPVERVTIAGGEYAVLLHTGP
YADLKTAYQWLYGDWLRHSGREAADAPPFELYLNTPMDTAPADLRTEIHLPLR
>Mature_292_residues
TPAETKTDPPAWVRYAASVARVHAYISAHLDGPLDLNRLAEVACLSPYHWQRVYRALYGESIVLTVRRMRIVRASEDLVC
TARPLDEIARRAGYGSTHAFARAFRDAYGVLPAQHRRTGTHRPIYPLQRGEADMFKHEVEIRRLPELRGYAVTHTGAYMK
VGDAFGRIGAWVGQHGLIGPGAKMIGIFYDDPDTTPEAQLRAKACFMPADGAPDVAPVPPVERVTIAGGEYAVLLHTGPY
ADLKTAYQWLYGDWLRHSGREAADAPPFELYLNTPMDTAPADLRTEIHLPLR

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=288, Percent_Identity=25, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 32598; Mature: 32466

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAETKTDPPAWVRYAASVARVHAYISAHLDGPLDLNRLAEVACLSPYHWQRVYRALYG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHC
ESIVLTVRRMRIVRASEDLVCTARPLDEIARRAGYGSTHAFARAFRDAYGVLPAQHRRTG
CHHEEHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCC
THRPIYPLQRGEADMFKHEVEIRRLPELRGYAVTHTGAYMKVGDAFGRIGAWVGQHGLIG
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHCCCCCCC
PGAKMIGIFYDDPDTTPEAQLRAKACFMPADGAPDVAPVPPVERVTIAGGEYAVLLHTGP
CCCEEEEEEECCCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEECCC
YADLKTAYQWLYGDWLRHSGREAADAPPFELYLNTPMDTAPADLRTEIHLPLR
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
TPAETKTDPPAWVRYAASVARVHAYISAHLDGPLDLNRLAEVACLSPYHWQRVYRALYG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHC
ESIVLTVRRMRIVRASEDLVCTARPLDEIARRAGYGSTHAFARAFRDAYGVLPAQHRRTG
CHHEEHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCC
THRPIYPLQRGEADMFKHEVEIRRLPELRGYAVTHTGAYMKVGDAFGRIGAWVGQHGLIG
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHCCCCCCC
PGAKMIGIFYDDPDTTPEAQLRAKACFMPADGAPDVAPVPPVERVTIAGGEYAVLLHTGP
CCCEEEEEEECCCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEECCC
YADLKTAYQWLYGDWLRHSGREAADAPPFELYLNTPMDTAPADLRTEIHLPLR
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]