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The map label for this gene is emrB [H]

Identifier: 116688071

GI number: 116688071

Start: 47883

End: 49445

Strand: Reverse

Name: emrB [H]

Synonym: Bcen2424_0047

Alternate gene names: 116688071

Gene position: 49445-47883 (Counterclockwise)

Preceding gene: 116688072

Following gene: 116688070

Centisome position: 1.42

GC content: 67.5

Gene sequence:

>1563_bases
ATGTCCGCCACCACGGCATCCGCCCCCTCCCCCGCTGCCGAACCGGCGCCGCTGACCGGCGGCGCGCTCGCACTGCTGAC
CGTCGGGCTCGCGCTCGGCACCTTCATGGAAGTGCTCGACACGTCGATCGCGAACGTCGCGGTGCCGACCATCTCGGGCA
GCCTCGGCGTCGCGACGAGCGAGGGCACGTGGGTGATTTCGTCGTACTCGGTCGCGTCCGCGATCGCGGTGCCGCTGACC
GGCTGGCTCGCGCGCCGGGTCGGCGAGGTGCGCCTGTTCACGCTGTCGGTGCTCGCGTTCACGATCGCGTCGGCGCTGTG
CGGCTTCGCGACCAACTTCGAGACGCTGATCGCGTTCCGGCTGCTGCAGGGCCTCGTGTCCGGGCCGATGGTGCCGCTGT
CGCAGACGATTCTGATGCGCAGCTACCCACCCGCGAAACGCGGGCTCGCGCTCGGGCTATGGGCGATGACGGTGATCGTC
GCGCCGATCTTCGGCCCGCTGCTGGGCGGCTGGATCAGCGACAACTACACGTGGCCGTGGATCTTCTACATCAACCTGCC
GATCGGCGTGTTCTCGGCGACCTGCGCGTATTTCCTGCTGCGCGGCCGCGAGACGAAGACGTCGAAGCAGCGGATCGACG
CGATCGGCCTCGCGCTGCTCGTGATCGGCGTGTCGTGCCTGCAGATGATGCTCGACCTCGGCAAGGACCGCGACTGGTTC
AACTCGACCTTCATCGTCGCGCTCGCGCTGATCGCGGTCGTGTCGCTCGCGTTCATGCTCGTGTGGGAGGCGACCGAGAA
GGAGCCGGTGGTCGACCTGTCGCTCTTCAAGGACCGCAACTTCGCGCTCGGCGCGCTGATCATCTCGTTCGGCTTCATGG
CGTTCTTCGGCTCGGTCGTGATCTTCCCGCTGTGGCTGCAGACGGTGATGGGCTACACGGCCGGCAAGGCCGGCCTCGCG
ACCGCGCCGGTCGGGCTGCTCGCGCTGGTGCTGTCGCCGCTGATCGGCCGCAACATGCACCGGCTCGACCTGCGGATGGT
CGCGAGCTTCGCGTTCATCGTGTTCGCGGGCGTGTCGCTGTGGAACGCGACGTTTACGCTCGACGTGCCGTTCAACCACG
TGATCCTGCCGCGGCTCGTGCAGGGCATCGGCGTCGCGTGCTTCTTCGTGCCGATGACGACGATCACGCTGTCGAGCATC
TCCGACGAGCGGCTCGCGAGTGCGTCGGGGCTGTCGAACTTCCTGCGCACGCTGTCCGGCGCGATCGGCACCGCGGCCAG
CTCGACGTTCTGGGAGAACGACGCGATCTACCATCATGCGCGGCTGTCGGAATCGGTGAGCATCTACGCGCAGAACACGA
CCGACTACCAGGGCGCGCTCGCGCAGCTCGGCATCGTCGGCCAGACGGCCAATGCGCAACTCAACCAGCTCGTCACGCAG
CAAGGCTTCATGATGGCGACCAACGACTTCTTCCATCTGTCGGCGGGCGTGTTCGTCGCGCTCGCGGCGCTCGTGTGGAT
CACGAAGCCGAAGAAGGGCGCAGGGCCCGCGATGGGGCATTGA

Upstream 100 bases:

>100_bases
CCCCTTTTCCTCTTTTTTATTCCACCTGGCCGGGCCGCGCTGCGTCGCGCGCCCGTGCGTTGTGCCGGCGCGTCGCGCGC
GCCGCGTCTAGGAGATTCCG

Downstream 100 bases:

>100_bases
GGGGGCGCGAGCAGCCCTGTATGCAGCCGACGACAGGTCTTGCGGGCATTCTCTATATGAACTAAGCTAGAACGCTGACT
GAACTTAGTTTATTTGAGGG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSATTASAPSPAAEPAPLTGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLT
GWLARRVGEVRLFTLSVLAFTIASALCGFATNFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIV
APIFGPLLGGWISDNYTWPWIFYINLPIGVFSATCAYFLLRGRETKTSKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
NSTFIVALALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGALIISFGFMAFFGSVVIFPLWLQTVMGYTAGKAGLA
TAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAGVSLWNATFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSI
SDERLASASGLSNFLRTLSGAIGTAASSTFWENDAIYHHARLSESVSIYAQNTTDYQGALAQLGIVGQTANAQLNQLVTQ
QGFMMATNDFFHLSAGVFVALAALVWITKPKKGAGPAMGH

Sequences:

>Translated_520_residues
MSATTASAPSPAAEPAPLTGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLT
GWLARRVGEVRLFTLSVLAFTIASALCGFATNFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIV
APIFGPLLGGWISDNYTWPWIFYINLPIGVFSATCAYFLLRGRETKTSKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
NSTFIVALALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGALIISFGFMAFFGSVVIFPLWLQTVMGYTAGKAGLA
TAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAGVSLWNATFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSI
SDERLASASGLSNFLRTLSGAIGTAASSTFWENDAIYHHARLSESVSIYAQNTTDYQGALAQLGIVGQTANAQLNQLVTQ
QGFMMATNDFFHLSAGVFVALAALVWITKPKKGAGPAMGH
>Mature_519_residues
SATTASAPSPAAEPAPLTGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLTG
WLARRVGEVRLFTLSVLAFTIASALCGFATNFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVA
PIFGPLLGGWISDNYTWPWIFYINLPIGVFSATCAYFLLRGRETKTSKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWFN
STFIVALALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGALIISFGFMAFFGSVVIFPLWLQTVMGYTAGKAGLAT
APVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAGVSLWNATFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSIS
DERLASASGLSNFLRTLSGAIGTAASSTFWENDAIYHHARLSESVSIYAQNTTDYQGALAQLGIVGQTANAQLNQLVTQQ
GFMMATNDFFHLSAGVFVALAALVWITKPKKGAGPAMGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=498, Percent_Identity=53.6144578313253, Blast_Score=533, Evalue=1e-153,
Organism=Escherichia coli, GI1788710, Length=514, Percent_Identity=47.0817120622568, Blast_Score=493, Evalue=1e-140,
Organism=Escherichia coli, GI1790195, Length=407, Percent_Identity=30.2211302211302, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788392, Length=400, Percent_Identity=29.5, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=25.8823529411765, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI87082320, Length=176, Percent_Identity=26.1363636363636, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=215, Percent_Identity=29.3023255813954, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=29.4573643410853, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=319, Percent_Identity=25.705329153605, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=27.3333333333333, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55312; Mature: 55181

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATTASAPSPAAEPAPLTGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEC
EGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLAFTIASALCGFATNFETLIAFR
CCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVAPIFGPLLGGWISDNYTWPW
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEE
IFYINLPIGVFSATCAYFLLRGRETKTSKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
EEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NSTFIVALALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGALIISFGFMAFFGSVV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHEECCCCCHHHHHHHHHHHHHHHHHHHH
IFPLWLQTVMGYTAGKAGLATAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAGVSL
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
WNATFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSISDERLASASGLSNFLRTLSG
HCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AIGTAASSTFWENDAIYHHARLSESVSIYAQNTTDYQGALAQLGIVGQTANAQLNQLVTQ
HHCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
QGFMMATNDFFHLSAGVFVALAALVWITKPKKGAGPAMGH
CCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SATTASAPSPAAEPAPLTGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEC
EGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLAFTIASALCGFATNFETLIAFR
CCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVAPIFGPLLGGWISDNYTWPW
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEE
IFYINLPIGVFSATCAYFLLRGRETKTSKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
EEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NSTFIVALALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGALIISFGFMAFFGSVV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHEECCCCCHHHHHHHHHHHHHHHHHHHH
IFPLWLQTVMGYTAGKAGLATAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAGVSL
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
WNATFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSISDERLASASGLSNFLRTLSG
HCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AIGTAASSTFWENDAIYHHARLSESVSIYAQNTTDYQGALAQLGIVGQTANAQLNQLVTQ
HHCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
QGFMMATNDFFHLSAGVFVALAALVWITKPKKGAGPAMGH
CCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]