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The map label for this gene is gyrB [H]

Identifier: 116688027

GI number: 116688027

Start: 3224

End: 5698

Strand: Direct

Name: gyrB [H]

Synonym: Bcen2424_0003

Alternate gene names: 116688027

Gene position: 3224-5698 (Clockwise)

Preceding gene: 116688026

Following gene: 116688028

Centisome position: 0.09

GC content: 62.14

Gene sequence:

>2475_bases
ATGAGTGAACAGCACAATTCGCAACCCGATAACAGCAGCTACGGCGCTTCGTCGATCCAGATCCTCGAAGGTCTGGAAGC
GGTGCGCAAGCGCCCCGGGATGTACATCGGCGACACGTCGGACGGCACCGGTCTGCACCACCTCGTGTTCGAGGTGCTCG
ACAACTCGATCGACGAAGCGTTGGCCGGGTACTGCAACGACATCCACGTGACGATTCACGCCGACAACTCGATTTCTGTG
ACCGACAACGGCCGCGGGATTCCGACCGACGTGAAGATGAACGACAAGCACGAGCCGAAGCGCAGCGCCGCCGAGATCGT
GATGACCGAGCTGCACGCCGGCGGCAAGTTCGACCAGAACAGCTACAAGGTGTCGGGCGGCCTGCACGGCGTGGGCGTGT
CGTGCGTGAACGCGCTGTCGAGCTGGCTGCGCCTCACCGTCCGCCGCGACGGCAAGAAGCGCTTCATGGAATTCCACCGC
GGCGTCGCGCAGGATCGCGTGCTCGAAGTGGTGGACGGCGTGGAAGTGTCGCCGATGCTCGTGACCGGCGACACCGAGAA
CCGCGGCACCGAAGTGCATTTCATGGCCGATCCGACCATTTTCGGCACGGTCGAGTATCACTACGACATTCTCGCGAAGC
GGATGCGCGAGCTTTCGTTCCTGAACAACGGCGTGCGGATTCGTCTGACCGACCTGCGCTCGGGCAAGGAAGACGATTTC
GCGTTCGCCGGCGGTGTGAAGGGCTTCGTCGAGTACATCAACAAGACGAAGACCAACCTGCACCCGACCGTGTTCTTCGC
CAACGGCGAGAAGGACGGCGTGGGCGTCGAAGTCGCGATGCAGTGGAACGACAGCTACAACGAGAACGTGCTGTGCTTCA
CGAACAACATTCCGCAGCGCGACGGCGGCACGCACCTGACCGGCCTGCGGGCCGCGATGACGCGCGTCATCAACAAGTAC
ATCACCGACAACGAAATCGCGAAGAAGGCGAAGGTCGAGACGACCGGCGACGACATGCGCGAAGGGCTGTCGTGCGTGCT
CTCCGTGAAGGTGCCGGAGCCGAAGTTCAGCTCGCAGACGAAGGACAAGCTGGTTTCTTCCGAGGTTCGTGCGCCGGTTG
AGGAAGTTGTGGCGAAGGCACTGGAAGAGTTCCTGCTGGAGACGCCGATCGACGCGAAGATCATCTGCGGGAAGATCGTC
GAAGCCGCGCGGGCGCGTGATGCTGCTCGGAAGGCCCGTGAGATGACGCGTCGGAAGGGTGTGCTCGATGGCGTCGGCCT
GCCCGGCAAGCTCGCGGACTGCCAGGAGAAAGACCCGGCGAAGTGCGAAATCTACATCGTCGAGGGTGACTCGGCAGGTG
GGTCGGCGAAGCAAGGGCGTGATCGCAAGTTCCAGGCGATTCTGCCGCTGCGCGGCAAGGTGCTGAACGTCGAGAAGGCG
CGCTACGACAAGCTGCTGTCGTCGGAGCAGATCGTCACGCTCGTGACCGCGCTTGGGTGCGGCATCGGCAAGGAAGACTA
CAACCTCGACAAGCTGCGCTATCACCGCATCATCATCATGACCGACGCGGACGTCGACGGTGCGCACATCCGGACGCTGC
TGCTCACGTTCCTGTATCGCCAGATGCCGGACATGATCGAGCGCGGGTACGTGTATATCGCGCAGCCGCCGCTTTACAAG
ATCAAGGCGGGCAAGGACGAGCGGTATCTGAAGGACGACGTCGAGCTCAACGCGCACATGCTGCGGTTGGCGCTGCAAGG
GTCGGAGCTGGTGCCGGGTGAGAATGCGGCGGCGATTTCGGGTGATGCGCTTGGGGAACTGGCGCGGTCGTATCTGCTGT
CGCAGAGCGTGATCGATCGGTTGAGCCGGTTGTATGACCCGGCTGCGCTGGAAGCGGTCATGGATGGCGTGGTGATCGAT
CTCTCCAATGAGGAGTCGACCGAGGCTTCGGCTAAGGCGCTGCATGCTGCGTTGCATGATGAAGCGCTGAAGAACGAAGT
GCGGGTGGTGCCTTCGTATGACGCGGTTCGCGAACAGCGGGCGCTGCATGTCGAGCGCACGCATCACGGCAACGTGCGCG
TTTCGGTCATCGACCAGGAATTCCAGCACACGGCGGATTATCAGCAGCTCGTGACCACCGCGAATACGTTCAAGGGGCTG
ATTGGGGAAGGTGCGGTCATCAAGCGCGGTGAGCGCAGCATGGCCGTGGCGGACTTCAAGAGCGCGATGAAGTGGCTGCT
GGCGGATGCTGAGCGGAACGTGTCGAAGCAACGCTATAAGGGGCTCGGCGAGATGAACCCCGAGCAGCTGTGGGAAACGA
CGATGGATCCGGCCGTGCGGCGTCTGCTGCGTGTGCAGATCGAAGACGCGATTGCTGCGGATGGGATCTTTACTACGCTG
ATGGGGGATGACGTCGAGCCGCGGCGGGCGTTTATCGAGTCGAATGCGTTGCGGGCGGGGAATATTGACGTTTAA

Upstream 100 bases:

>100_bases
GGCACTTGGGGCGGGCCAGGAAACTGGAGCGGCCCGGCGATTTCTCGCTGAAACGCACCGCGCCGCCACGAGAGTGGCCC
CGCAGAACCGGAAGATATCC

Downstream 100 bases:

>100_bases
CTCTGTATGTAGCAGGACCCATAAGGTGAGATTTTGGGAAGCATCCACTGATACATGTATTACTGGCTGCGTTCCGATAA
GTCGGATTTATGGCGCTTAG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 824; Mature: 823

Protein sequence:

>824_residues
MSEQHNSQPDNSSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGYCNDIHVTIHADNSISV
TDNGRGIPTDVKMNDKHEPKRSAAEIVMTELHAGGKFDQNSYKVSGGLHGVGVSCVNALSSWLRLTVRRDGKKRFMEFHR
GVAQDRVLEVVDGVEVSPMLVTGDTENRGTEVHFMADPTIFGTVEYHYDILAKRMRELSFLNNGVRIRLTDLRSGKEDDF
AFAGGVKGFVEYINKTKTNLHPTVFFANGEKDGVGVEVAMQWNDSYNENVLCFTNNIPQRDGGTHLTGLRAAMTRVINKY
ITDNEIAKKAKVETTGDDMREGLSCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEEVVAKALEEFLLETPIDAKIICGKIV
EAARARDAARKAREMTRRKGVLDGVGLPGKLADCQEKDPAKCEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKVLNVEKA
RYDKLLSSEQIVTLVTALGCGIGKEDYNLDKLRYHRIIIMTDADVDGAHIRTLLLTFLYRQMPDMIERGYVYIAQPPLYK
IKAGKDERYLKDDVELNAHMLRLALQGSELVPGENAAAISGDALGELARSYLLSQSVIDRLSRLYDPAALEAVMDGVVID
LSNEESTEASAKALHAALHDEALKNEVRVVPSYDAVREQRALHVERTHHGNVRVSVIDQEFQHTADYQQLVTTANTFKGL
IGEGAVIKRGERSMAVADFKSAMKWLLADAERNVSKQRYKGLGEMNPEQLWETTMDPAVRRLLRVQIEDAIAADGIFTTL
MGDDVEPRRAFIESNALRAGNIDV

Sequences:

>Translated_824_residues
MSEQHNSQPDNSSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGYCNDIHVTIHADNSISV
TDNGRGIPTDVKMNDKHEPKRSAAEIVMTELHAGGKFDQNSYKVSGGLHGVGVSCVNALSSWLRLTVRRDGKKRFMEFHR
GVAQDRVLEVVDGVEVSPMLVTGDTENRGTEVHFMADPTIFGTVEYHYDILAKRMRELSFLNNGVRIRLTDLRSGKEDDF
AFAGGVKGFVEYINKTKTNLHPTVFFANGEKDGVGVEVAMQWNDSYNENVLCFTNNIPQRDGGTHLTGLRAAMTRVINKY
ITDNEIAKKAKVETTGDDMREGLSCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEEVVAKALEEFLLETPIDAKIICGKIV
EAARARDAARKAREMTRRKGVLDGVGLPGKLADCQEKDPAKCEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKVLNVEKA
RYDKLLSSEQIVTLVTALGCGIGKEDYNLDKLRYHRIIIMTDADVDGAHIRTLLLTFLYRQMPDMIERGYVYIAQPPLYK
IKAGKDERYLKDDVELNAHMLRLALQGSELVPGENAAAISGDALGELARSYLLSQSVIDRLSRLYDPAALEAVMDGVVID
LSNEESTEASAKALHAALHDEALKNEVRVVPSYDAVREQRALHVERTHHGNVRVSVIDQEFQHTADYQQLVTTANTFKGL
IGEGAVIKRGERSMAVADFKSAMKWLLADAERNVSKQRYKGLGEMNPEQLWETTMDPAVRRLLRVQIEDAIAADGIFTTL
MGDDVEPRRAFIESNALRAGNIDV
>Mature_823_residues
SEQHNSQPDNSSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGYCNDIHVTIHADNSISVT
DNGRGIPTDVKMNDKHEPKRSAAEIVMTELHAGGKFDQNSYKVSGGLHGVGVSCVNALSSWLRLTVRRDGKKRFMEFHRG
VAQDRVLEVVDGVEVSPMLVTGDTENRGTEVHFMADPTIFGTVEYHYDILAKRMRELSFLNNGVRIRLTDLRSGKEDDFA
FAGGVKGFVEYINKTKTNLHPTVFFANGEKDGVGVEVAMQWNDSYNENVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYI
TDNEIAKKAKVETTGDDMREGLSCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEEVVAKALEEFLLETPIDAKIICGKIVE
AARARDAARKAREMTRRKGVLDGVGLPGKLADCQEKDPAKCEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKVLNVEKAR
YDKLLSSEQIVTLVTALGCGIGKEDYNLDKLRYHRIIIMTDADVDGAHIRTLLLTFLYRQMPDMIERGYVYIAQPPLYKI
KAGKDERYLKDDVELNAHMLRLALQGSELVPGENAAAISGDALGELARSYLLSQSVIDRLSRLYDPAALEAVMDGVVIDL
SNEESTEASAKALHAALHDEALKNEVRVVPSYDAVREQRALHVERTHHGNVRVSVIDQEFQHTADYQQLVTTANTFKGLI
GEGAVIKRGERSMAVADFKSAMKWLLADAERNVSKQRYKGLGEMNPEQLWETTMDPAVRRLLRVQIEDAIAADGIFTTLM
GDDVEPRRAFIESNALRAGNIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=550, Percent_Identity=24.7272727272727, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI19913408, Length=572, Percent_Identity=24.4755244755245, Blast_Score=112, Evalue=2e-24,
Organism=Escherichia coli, GI48994957, Length=819, Percent_Identity=59.5848595848596, Blast_Score=956, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=559, Percent_Identity=37.9248658318426, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535065, Length=602, Percent_Identity=23.7541528239203, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=520, Percent_Identity=25.1923076923077, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI212645657, Length=159, Percent_Identity=28.9308176100629, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=616, Percent_Identity=24.6753246753247, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17136538, Length=555, Percent_Identity=24.1441441441441, Blast_Score=115, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 91132; Mature: 91000

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQHNSQPDNSSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
LAGYCNDIHVTIHADNSISVTDNGRGIPTDVKMNDKHEPKRSAAEIVMTELHAGGKFDQN
HHHCCCEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SYKVSGGLHGVGVSCVNALSSWLRLTVRRDGKKRFMEFHRGVAQDRVLEVVDGVEVSPML
CEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHCCCCCCEEE
VTGDTENRGTEVHFMADPTIFGTVEYHYDILAKRMRELSFLNNGVRIRLTDLRSGKEDDF
EECCCCCCCCEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
AFAGGVKGFVEYINKTKTNLHPTVFFANGEKDGVGVEVAMQWNDSYNENVLCFTNNIPQR
EECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCEEEEECCCCCC
DGGTHLTGLRAAMTRVINKYITDNEIAKKAKVETTGDDMREGLSCVLSVKVPEPKFSSQT
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHCCEEEEEEECCCCCCCCHH
KDKLVSSEVRAPVEEVVAKALEEFLLETPIDAKIICGKIVEAARARDAARKAREMTRRKG
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VLDGVGLPGKLADCQEKDPAKCEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKVLNVEKA
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEECHHH
RYDKLLSSEQIVTLVTALGCGIGKEDYNLDKLRYHRIIIMTDADVDGAHIRTLLLTFLYR
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHH
QMPDMIERGYVYIAQPPLYKIKAGKDERYLKDDVELNAHMLRLALQGSELVPGENAAAIS
HHHHHHHCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEC
GDALGELARSYLLSQSVIDRLSRLYDPAALEAVMDGVVIDLSNEESTEASAKALHAALHD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHH
EALKNEVRVVPSYDAVREQRALHVERTHHGNVRVSVIDQEFQHTADYQQLVTTANTFKGL
HHHHCCEEECCCHHHHHHHHHHEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
IGEGAVIKRGERSMAVADFKSAMKWLLADAERNVSKQRYKGLGEMNPEQLWETTMDPAVR
HCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RLLRVQIEDAIAADGIFTTLMGDDVEPRRAFIESNALRAGNIDV
HHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SEQHNSQPDNSSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
LAGYCNDIHVTIHADNSISVTDNGRGIPTDVKMNDKHEPKRSAAEIVMTELHAGGKFDQN
HHHCCCEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SYKVSGGLHGVGVSCVNALSSWLRLTVRRDGKKRFMEFHRGVAQDRVLEVVDGVEVSPML
CEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHCCCCCCEEE
VTGDTENRGTEVHFMADPTIFGTVEYHYDILAKRMRELSFLNNGVRIRLTDLRSGKEDDF
EECCCCCCCCEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
AFAGGVKGFVEYINKTKTNLHPTVFFANGEKDGVGVEVAMQWNDSYNENVLCFTNNIPQR
EECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCEEEEECCCCCC
DGGTHLTGLRAAMTRVINKYITDNEIAKKAKVETTGDDMREGLSCVLSVKVPEPKFSSQT
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHCCEEEEEEECCCCCCCCHH
KDKLVSSEVRAPVEEVVAKALEEFLLETPIDAKIICGKIVEAARARDAARKAREMTRRKG
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VLDGVGLPGKLADCQEKDPAKCEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKVLNVEKA
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEECHHH
RYDKLLSSEQIVTLVTALGCGIGKEDYNLDKLRYHRIIIMTDADVDGAHIRTLLLTFLYR
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHH
QMPDMIERGYVYIAQPPLYKIKAGKDERYLKDDVELNAHMLRLALQGSELVPGENAAAIS
HHHHHHHCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEC
GDALGELARSYLLSQSVIDRLSRLYDPAALEAVMDGVVIDLSNEESTEASAKALHAALHD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHH
EALKNEVRVVPSYDAVREQRALHVERTHHGNVRVSVIDQEFQHTADYQQLVTTANTFKGL
HHHHCCEEECCCHHHHHHHHHHEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
IGEGAVIKRGERSMAVADFKSAMKWLLADAERNVSKQRYKGLGEMNPEQLWETTMDPAVR
HCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RLLRVQIEDAIAADGIFTTLMGDDVEPRRAFIESNALRAGNIDV
HHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1906260 [H]