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The map label for this gene is 116626028

Identifier: 116626028

GI number: 116626028

Start: 8774415

End: 8776121

Strand: Reverse

Name: 116626028

Synonym: Acid_6986

Alternate gene names: NA

Gene position: 8776121-8774415 (Counterclockwise)

Preceding gene: 116626035

Following gene: 116626026

Centisome position: 88.06

GC content: 61.92

Gene sequence:

>1707_bases
ATGACCCATCCAAAGCGATCTCACGCAAAAATACTGCTCACGTCCGTATTTGGGCCCTATGCGCAGGACGACGAATACGG
CAGCCGGGCCATCAACCCGATGGAGCTGTACCACAACCAGGTGACGCGGGGACAGGGATCGTTTTCGCTGCGGCGGTTCC
ACCGGTCATGGGGAATCCTGATGATTCAGGCGAATATTTCGGCGCCTTGCGCGGTGCTGGATTTCCCGACGCGGGAGACG
TTCGCGCAGGAACTCAAGGCGCACCAGTACGACATCGTGGGGATCTCGGCGATTATCGTCAATACCGGCAAGGTTCAGGA
GATGTGCCGGATGGTGCGGGAGCTTTCGCCGCGAAGCACGATCGTGGTGGGCGGGCATGTGGCGGCAATTCCGGGCGTGG
AGAAGATGCTGGATGCCGATCATATTGTGAAGGGCGACGGGATCGCGTGGATGCGCGGGTTCCTGGGGGAAGAGGTGGAG
GCGCCGATCCAGCATCCGGATTTATCGTCGGGCTTCGACCTGCGGGTGATGGGGGTCAAGCTGCCGGAGACCGAGGACAC
GGCGGCGACGATCATTTCGTCGGTGGGGTGCCCGATGGGGTGCAACTTCTGTACGACGTCGGCATTCTTCGGCGGCAAGG
GGAAGATCCTCAATTTCTACGCGACGGGCGAGGAACTTTTCAAGCTGATGGAGAAGGCGGAGGAGACGCGGGGGACTCAC
AACTTCTTCATCATGGACGAGAATTTCCTGCTGCAGAAGAAGCGGGTGATGGAGCTCGTTACGCTGATGAAGCGGGCGGG
CAAGAGCTGGGGCTTCAACATTTTCTCGTCGGCGAATGCGATTGCGAAGTACACCTATGAGGAACTGGTGGAGATCGGGA
TCGCGTCGATCTGGCTGGGGTTGGAATCGCCGAAGTCGAATTATTCGAAGCTGGACGGCACGGATACGATGAAGCTCTCG
CGGGAACTGCGGGCGCACGGGATCGTGCTGCTGGGATCGACGATCGTGGGGCTGGAGCATCATACTCCGGCGAACATCGA
TGAAGAGATCGAGCATGCGGTGGCGCATGAGACGGACCTGCATCAATTCATGCTGTACACGCCGGTTCCGGGGACGCCGC
TGTATTTCGAGATGGCGGAGCAGGGGCGGCTGCTGGATGTGAACCTGGCGGACATCCACGGGCAGCACGCGTTCAATTTC
GAGCATGCGGCGATCTCGCGGGAGCAATCGACGCGGTCGCTGGCGAGGGCCTTTGAGCGGGATTTCGAGCGCAACGGCCC
GAGCCTGTTCCGGATTTGCCGGACGATGTTCCGGGGATGGATGCGGTATAAGAATCACCCCGATCGTCGGGTGCGCGAGC
GGTTCGGGCGCGATGCGAAGGTGCTGCGGCATGCGTGGGTGGGGATGCTGCAGGGGATGGAATACCGGCTGCGGGGATCG
AACCCGGCGATCGCGATGCAGATCGGCGAGCTGCGTAAGGAGATGCAGCGCGAGTTCGGGCTAATCGCGGTGGCGGCGGC
GAAGGTACTGGGTCCGGTGTTGTGGTGGACGAGCCTGCGGGAGGAGAAGCGGCTGGCGCGGGGGAAGAGCTATGAGCCGG
CGACGATGATCGACCGGCGCAATTGGGAAGCGGTGGGCGAGGCGGCGCCGATGCCGGGGATGGCGGAAGTGGAGTGTCCG
GCGGAGTCAGGGAGTTACGGAGACTGA

Upstream 100 bases:

>100_bases
TGGGCCATTTGAGACAATCAGGTCGGCGACACTACTATTACAGTCCGCAGATGTTCTTTTTGCTACGCTTCAGTATTAAT
AGGCTCTCTTTATCCTCTAT

Downstream 100 bases:

>100_bases
GCGGGCGGCCCACTCGGTGTTGAAGCGTTCGCGGGAGACGAAGGATTTTTGGGTGCCGGGGCCGAGGGTGGAGAGGGCCG
CGTAAAGATTGGCGCGGGCG

Product: radical SAM domain-containing protein

Products: 5'-deoxyadenosine; methionine; flavodoxin; formate acetyltransferase-glycine-2-yl radical

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MTHPKRSHAKILLTSVFGPYAQDDEYGSRAINPMELYHNQVTRGQGSFSLRRFHRSWGILMIQANISAPCAVLDFPTRET
FAQELKAHQYDIVGISAIIVNTGKVQEMCRMVRELSPRSTIVVGGHVAAIPGVEKMLDADHIVKGDGIAWMRGFLGEEVE
APIQHPDLSSGFDLRVMGVKLPETEDTAATIISSVGCPMGCNFCTTSAFFGGKGKILNFYATGEELFKLMEKAEETRGTH
NFFIMDENFLLQKKRVMELVTLMKRAGKSWGFNIFSSANAIAKYTYEELVEIGIASIWLGLESPKSNYSKLDGTDTMKLS
RELRAHGIVLLGSTIVGLEHHTPANIDEEIEHAVAHETDLHQFMLYTPVPGTPLYFEMAEQGRLLDVNLADIHGQHAFNF
EHAAISREQSTRSLARAFERDFERNGPSLFRICRTMFRGWMRYKNHPDRRVRERFGRDAKVLRHAWVGMLQGMEYRLRGS
NPAIAMQIGELRKEMQREFGLIAVAAAKVLGPVLWWTSLREEKRLARGKSYEPATMIDRRNWEAVGEAAPMPGMAEVECP
AESGSYGD

Sequences:

>Translated_568_residues
MTHPKRSHAKILLTSVFGPYAQDDEYGSRAINPMELYHNQVTRGQGSFSLRRFHRSWGILMIQANISAPCAVLDFPTRET
FAQELKAHQYDIVGISAIIVNTGKVQEMCRMVRELSPRSTIVVGGHVAAIPGVEKMLDADHIVKGDGIAWMRGFLGEEVE
APIQHPDLSSGFDLRVMGVKLPETEDTAATIISSVGCPMGCNFCTTSAFFGGKGKILNFYATGEELFKLMEKAEETRGTH
NFFIMDENFLLQKKRVMELVTLMKRAGKSWGFNIFSSANAIAKYTYEELVEIGIASIWLGLESPKSNYSKLDGTDTMKLS
RELRAHGIVLLGSTIVGLEHHTPANIDEEIEHAVAHETDLHQFMLYTPVPGTPLYFEMAEQGRLLDVNLADIHGQHAFNF
EHAAISREQSTRSLARAFERDFERNGPSLFRICRTMFRGWMRYKNHPDRRVRERFGRDAKVLRHAWVGMLQGMEYRLRGS
NPAIAMQIGELRKEMQREFGLIAVAAAKVLGPVLWWTSLREEKRLARGKSYEPATMIDRRNWEAVGEAAPMPGMAEVECP
AESGSYGD
>Mature_567_residues
THPKRSHAKILLTSVFGPYAQDDEYGSRAINPMELYHNQVTRGQGSFSLRRFHRSWGILMIQANISAPCAVLDFPTRETF
AQELKAHQYDIVGISAIIVNTGKVQEMCRMVRELSPRSTIVVGGHVAAIPGVEKMLDADHIVKGDGIAWMRGFLGEEVEA
PIQHPDLSSGFDLRVMGVKLPETEDTAATIISSVGCPMGCNFCTTSAFFGGKGKILNFYATGEELFKLMEKAEETRGTHN
FFIMDENFLLQKKRVMELVTLMKRAGKSWGFNIFSSANAIAKYTYEELVEIGIASIWLGLESPKSNYSKLDGTDTMKLSR
ELRAHGIVLLGSTIVGLEHHTPANIDEEIEHAVAHETDLHQFMLYTPVPGTPLYFEMAEQGRLLDVNLADIHGQHAFNFE
HAAISREQSTRSLARAFERDFERNGPSLFRICRTMFRGWMRYKNHPDRRVRERFGRDAKVLRHAWVGMLQGMEYRLRGSN
PAIAMQIGELRKEMQREFGLIAVAAAKVLGPVLWWTSLREEKRLARGKSYEPATMIDRRNWEAVGEAAPMPGMAEVECPA
ESGSYGD

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: 1.97.1.4

Molecular weight: Translated: 63848; Mature: 63717

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPKRSHAKILLTSVFGPYAQDDEYGSRAINPMELYHNQVTRGQGSFSLRRFHRSWGIL
CCCCCCCCHHEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCEE
MIQANISAPCAVLDFPTRETFAQELKAHQYDIVGISAIIVNTGKVQEMCRMVRELSPRST
EEEECCCCCEEEEECCCHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCE
IVVGGHVAAIPGVEKMLDADHIVKGDGIAWMRGFLGEEVEAPIQHPDLSSGFDLRVMGVK
EEECCCEEECCCHHHHHCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEE
LPETEDTAATIISSVGCPMGCNFCTTSAFFGGKGKILNFYATGEELFKLMEKAEETRGTH
CCCCCHHHHHHHHHCCCCCCCCHHHHCCEECCCCCEEEEEECHHHHHHHHHHHHHCCCCC
NFFIMDENFLLQKKRVMELVTLMKRAGKSWGFNIFSSANAIAKYTYEELVEIGIASIWLG
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHEEE
LESPKSNYSKLDGTDTMKLSRELRAHGIVLLGSTIVGLEHHTPANIDEEIEHAVAHETDL
CCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCEEEECCCCCCCCHHHHHHHHHHCCCCC
HQFMLYTPVPGTPLYFEMAEQGRLLDVNLADIHGQHAFNFEHAAISREQSTRSLARAFER
CCEEEEECCCCCCEEEEECCCCCEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DFERNGPSLFRICRTMFRGWMRYKNHPDRRVRERFGRDAKVLRHAWVGMLQGMEYRLRGS
HHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCC
NPAIAMQIGELRKEMQREFGLIAVAAAKVLGPVLWWTSLREEKRLARGKSYEPATMIDRR
CCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
NWEAVGEAAPMPGMAEVECPAESGSYGD
CCHHHCCCCCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
THPKRSHAKILLTSVFGPYAQDDEYGSRAINPMELYHNQVTRGQGSFSLRRFHRSWGIL
CCCCCCCHHEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCEE
MIQANISAPCAVLDFPTRETFAQELKAHQYDIVGISAIIVNTGKVQEMCRMVRELSPRST
EEEECCCCCEEEEECCCHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCE
IVVGGHVAAIPGVEKMLDADHIVKGDGIAWMRGFLGEEVEAPIQHPDLSSGFDLRVMGVK
EEECCCEEECCCHHHHHCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEE
LPETEDTAATIISSVGCPMGCNFCTTSAFFGGKGKILNFYATGEELFKLMEKAEETRGTH
CCCCCHHHHHHHHHCCCCCCCCHHHHCCEECCCCCEEEEEECHHHHHHHHHHHHHCCCCC
NFFIMDENFLLQKKRVMELVTLMKRAGKSWGFNIFSSANAIAKYTYEELVEIGIASIWLG
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHEEE
LESPKSNYSKLDGTDTMKLSRELRAHGIVLLGSTIVGLEHHTPANIDEEIEHAVAHETDL
CCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCEEEECCCCCCCCHHHHHHHHHHCCCCC
HQFMLYTPVPGTPLYFEMAEQGRLLDVNLADIHGQHAFNFEHAAISREQSTRSLARAFER
CCEEEEECCCCCCEEEEECCCCCEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DFERNGPSLFRICRTMFRGWMRYKNHPDRRVRERFGRDAKVLRHAWVGMLQGMEYRLRGS
HHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCC
NPAIAMQIGELRKEMQREFGLIAVAAAKVLGPVLWWTSLREEKRLARGKSYEPATMIDRR
CCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
NWEAVGEAAPMPGMAEVECPAESGSYGD
CCHHHCCCCCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; dihydroflavodoxin; formate acetyltransferase-glycine

Specific reaction: S-adenosyl-L-methionine + dihydroflavodoxin + formate acetyltransferase-glycine = 5'-deoxyadenosine + methionine + flavodoxin + formate acetyltransferase-glycine-2-yl radical

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]